Liquid Chromatography (LC/HPLC)

The analogy that electrons flowing in wires is like water flowing through a tube can be remarkably effective. In this installment, the basics of that analogy are discussed.

This article describes newly introduced high-performance liquid chromatography (HPLC), mass spectrometry (MS), chromatography data systems (CDS), and related products in 2023–2024. It summarizes their technical specifications and significant user benefits.

This article examinations the determination of retention time and why it's important.

In this interview, Kevin Schug discusses his work using untargeted analysis and differentiation of psilocybin mushroom strains.

Here are the top five articles that the editors of LCGC International published this week.

The 26th Norwegian Symposium on Chromatography was held 21–23 January 2024. The symposium has strong traditions in the Norwegian separation science community, serving as a forum for excellent scientific talks, networking, and social events.

A study has used gas chromatography coupled to a time-of-flight mass spectrometer to build an electron ionization mass spectra database of more than 250 chemicals classified as either volatile or semi-volatile compounds. An additional, confirmatory layer of liquid chromatography–mass spectrometry analysis was subsequently performed.

Scientists from Auburn University recently investigated how natural deep eutectic solvents (NADES) can be incorporated into high-performance liquid chromatography (HPLC) mobile phases.

Scientists recently tested a new system for detecting and extracting favipiravir from plasma samples.

Scientists from the Affiliated Hospital of Putian University recently analyzed the dysregulation of lipid metabolism in patients suffering from chronic hepatitis B patients.

This week, LCGC International published a variety of articles on the hottest topics in chromatography and beyond. Below, we’ve highlighted some of the most popular articles, according to our readers. Happy reading!

In this edition of “Inside the Laboratory,” Kevin Schug, PhD, a full professor of chemistry at the University of Texas at Arlington, discusses his laboratory’s group work in environmental monitoring around water and soil quality near oil and gas extraction, using techniques such as liquid chromatography (LC), gas chromatography (GC), supercritical fluid chromatography (SFC), and coupling these techniques with mass spectrometry (MS).

In a recent study from the GITAM School of Pharmacy, two scientists used different liquid chromatography techniques to assess the properties of ozenoxacin (OXC) and its degradation products.

In a recent study out of Boehringer Ingelheim Animal Health USA Inc. in North Brunswick, New Jersey, scientists investigated cloruson and its related substances using an original ion-paired reversed phase ultraperformance liquid chromatography (IP-UHPLC) method.

In a recent study out of Central South University in Changsha, China, scientists tested different cosine similarity algorithms to test for illegal adulterants in drugs.

Here are the top five articles that the editors of LCGC International published this week.

In a recent study, scientists tested how effective dynorphin 1-17 peptides can be in treating chronic rhinosinusitis with nasal polyps (CRSwNP).

In this series from LCGC, we go on location to the Grinias Research Lab at Rowan University and speak to Professor James Grinias about his background, teaching philosophy, students, and lab equipment.

A recent study dissects a Chinese herbal medicine to determine why it is effective in treating polycystic ovary syndrome (PCOS).

A group of Canadian scientists recently measured how effective different solutions are in measuring ochratoxin A levels in flour samples.

At Pittcon 2024, LCGC International editor Patrick Lavery sat down with Martina Catani of the University of Ferrara to talk about the work her laboratory group is conducting.

Scientists from Hangzhou, China used ultra-performance liquid chromatography mass spectrometry and non-targeted metabolomics studies to help find treatments for colorectal cancer.

At Pittcon 2024, LCGC International editor Patrick Lavery sat down with Wolfgang Lindner of the University of Vienna to talk about his career in liquid chromatography (LC).

The concept of gradient delay volume (GDV) in liquid chromatography (LC) poses challenges for both beginners and experienced practitioners. The GDV, which affects the arrival time of mobile phase composition changes at the column inlet, can have a significant impact on method throughput, influencing the time required for mobile phase changes at both the beginning and end of the LC method. Different pump designs and column characteristics affect efficient use of the available analysis time, as well as overall throughput. Notably, achieving repeatable equilibration, rather than full equilibration of LC columns following mobile phase gradients, is often sufficient for many LC applications, which can also be leveraged to increase method throughput.

Scientists from Murcia, Spain recently created a new method for mycotoxin screening in human tissues using dispersive liquid–liquid microextraction and liquid chromatography–mass spectrometry.

Sara E. Kern and her team of the U.S. Food and Drug Administration (FDA) tested different analysis methods for rapidly screening for poppy seeds in food samples.

A session focusing on important developments in high-throughput analysis in the pharmaceutical sector using LC–MS kicked off Monday morning at Pittcon 2024.

Alyssa Sanchez of Florida International University presented her research on profiling the decomposition of heroin from South America, Mexico, Southwest and Southeast Asia.

Lidia Montero and Miguel Herrero from the Institute of Food Science Research in Spain discuss the evolving role of comprehensive two-dimensional liquid chromatography (LC×LC) and multi-second dimension LC×LC (multi-2D LC×LC) in food analysis.

The 18th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology will take place from 28–31 May 2024 in Leuven, Belgium.