Srinivasa Rao, Kelly Flook, Yury Agroskin, and Chris Pohl, Dionex Corporation
The new 1 mm i.d. ProSwift® strong cation-exchange (SCX) column is a polymethacrylate-based monolithic column with sulfonate functionality (1). The column is available in 1 × 50 mm format and is used for high-resolution separation of proteins.
ExperimentalDionex ICS-3000 LC system with DP pump, VWD absorbance detector, AS auto-sampler, and TCC-100 thermostatted column compartment was used.
Chromatography was controlled by the Chromeleon® Chromatography Data System.
Standard proteins, Naja naja and Russell's viper snake venoms, and other analytical-grade chemicals were obtained from Sigma.
Results and Discussion
Earlier, reversed-phase, weak and strong anion-exchange, and weak cation-exchange monolithic columns were introduced in a 1 mm i.d. format for protein separations. The new ProSwift SCX-1S column has a pore structure designed for efficient separation of proteins at elevated linear velocities. Due to its low operating backpressure, flow rates of up to 0.3 mL/min (>6 mm/s) can be employed while maintaining high resolution. In addition, chromatography using ProSwift SCX-1S columns can be performed on standard HPLC systems with only minor plumbing changes to minimize instrument delay volumes. Operation at reduced flow rates minimizes consumption of sample and eluents, greatly reducing the cost of analysis.
The ProSwift SCX-1S, 1 mm i.d. column provides high-resolution and cost-effective separation of simple and complex protein mixtures. The high loading capacity of the column makes it useful for both one-dimensional and two-dimensional chromatography platforms.
PEEK is a trademark of Victrex PLC.
Chromeleon and ProSwift are registered trademarks of Dionex Corporation.
(1) ProSwift IEX manual and ProSwift IEX data sheet are available at http://www.dionex.com/.
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