Omega-3 and -6 fatty acids are essential compounds required for normal growth. Omega-3 consumption is purported to have a number of health benefits, e.g., cancer prevention, cardiovascular disease prevention, and improved immune function. Although both omega-3 and -6 fatty acids can give rise to eicosanoid-signaling molecules (prostaglandins, prostacyclins, thromboxanes, and leukotrienes), the omega-6 eicosanoids are generally proinflammatory. A person's diet requires a balance of omega fatty acids at suitable levels.
Omega lipids found in foods are commonly determined after extraction, hydrolysis, and derivatization for measurement by gas chromatography (GC). Analyte derivatization can adversely affect temperature-sensitive functional groups on specialized lipids. High-performance liquid chromatography (HPLC) with ultraviolet detection at low wavelengths limits solvent selection and increases the likelihood of matrix interference.
Conditions and Sample Preparation
The experimental setup and sample preparation procedures are described in Poster Note LPN 2931-02, Thermo Fisher Scientific, Inc. (formerly Dionex Corp.).
After saponification to release the fatty acids, samples were neutralized and directly analyzed for omega-3, -6, and -9 fatty acids using this HPLC method with charged aerosol detection. The method can be used to analyze omega fatty acids from a variety of sources. The mobile phase is compatible with mass spectrometry, allowing the possibility of identifying unknown analytes.
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Thermo Fisher Scientific, Inc. (formerly Dionex Corp.)