Steroids represent a chemically distinct class of hormones with wide-ranging biological functions. Synthetic derivatives of endogenous steroid prototypes are used medically in birth control and in the treatment of asthma, arthritis, inflammation, and osteoporosis.
Table I: Experimental conditions
Steroids share a characteristic, polycyclic structure and have varying degrees of lipophilicity (log P). In this study, a reversed-phase HPLC method was developed for separation of five steroid hormones with partition coefficients ranging from 1.47 (cortisone) to 4.5 (pregnenolone) on a Hamilton PRP-C18 HPLC column.
Table II: Compounds
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Figure 1: Separation of five steroids on a 4.6 × 150 mm PRP-C18.
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