Separation of Five Steroids on a C18-Functionalized Polymeric Reversed-Phase HPLC Column (PRP-C18)

Jun 01, 2014


Table I: Experimental conditions
Steroids represent a chemically distinct class of hormones with wide-ranging biological functions. Synthetic derivatives of endogenous steroid prototypes are used medically in birth control and in the treatment of asthma, arthritis, inflammation, and osteoporosis.


Table II: Compounds
Steroids share a characteristic, polycyclic structure and have varying degrees of lipophilicity (log P). In this study, a reversed-phase HPLC method was developed for separation of five steroid hormones with partition coefficients ranging from 1.47 (cortisone) to 4.5 (pregnenolone) on a Hamilton PRP-C18 HPLC column.


Figure 1: Separation of five steroids on a 4.6 × 150 mm PRP-C18.

© 2014 Hamilton Company. All rights reserved. All trademarks are owned and/or registered by Hamilton Company in the U.S. and/or other countries. 04/2014 Lit. No. L80086 Rev. B




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