UHPLC Method Development and Modelling in the Framework of Quality by Design

Apr 08, 2014
Volume 10, Issue 6

The goals in ultrahigh-pressure liquid chromatography (UHPLC) method development are to first find the best separation, second find the best column, and third find the most robust method in a multifactorial Design Space. Trial and error methods are not sufficient anymore and solid science based on Quality by Design (QbD) principles is required.

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