The size, shape and structure of therapeutic nanoparticles are critical for effective drug delivery. Effective ad robust characterization for these qualities is achieved by combining asymmetric-flow field-flow-fractionation (AF4) with multi-angle and dynamic light scattering (MALS and DLS). This note describes the technology and presents examples of liposome- and nanolipid complex-based therapeutics.

The size, shape and structure of therapeutic nanoparticles are critical for effective drug delivery. Effective ad robust characterization for these qualities is achieved by combining asymmetric-flow field-flow-fractionation (AF4) with multi-angle and dynamic light scattering (MALS and DLS). This note describes the technology and presents examples of liposome- and nanolipid complex-based therapeutics.

Ligand binding assays (LBAs) used for large molecule drug development usually necessitate conjugation of a drug to one or more different tags. To help ensure that LBAs utilizing conjugated drug reagents perform consistently over time and provide high quality data, each lot of conjugated material should be characterized functionally and biophysically prior to being incorporated into the LBA.

Ligand binding assays (LBAs) used for large molecule drug development usually necessitate conjugation of a drug to one or more different tags. To help ensure that LBAs utilizing conjugated drug reagents perform consistently over time and provide high quality data, each lot of conjugated material should be characterized functionally and biophysically prior to being incorporated into the LBA.

Ligand Binding Assays (LBAs) used for large molecule drug development often utilize proteins conjugated to various tags. To ensure that these conjugated protein reagents perform consistently over time and provide high quality data, each lot of conjugated material should be characterized functionally and biophysically. This application note, contributed by scientists at Genentech (South San Francisco, CA), discusses the advantages of SEC-MALS for this application and the impact of aggregated reagents on assays used to detect anti-drug antibodies.

Ligand Binding Assays (LBAs) used for large molecule drug development often utilize proteins conjugated to various tags. To ensure that these conjugated protein reagents perform consistently over time and provide high quality data, each lot of conjugated material should be characterized functionally and biophysically. This application note, contributed by scientists at Genentech (South San Francisco, CA), discusses the advantages of SEC-MALS for this application and the impact of aggregated reagents on assays used to detect anti-drug antibodies.

Branching affects important mechanical, rheological and thermal properties of polymers. Quantitative information about branching topology is vital for developing novel polymer-based materials and for understanding polymerization processes. This article explains the advantages of light scattering coupled to SEC and field-flow fractionation for characterizing branching across a wide spectrum of polymers.

Branching affects important mechanical, rheological, and thermal properties of polymers. Quantitative information about branching topology is vital for developing novel polymer-based materials and for understanding polymerization processes. This article explains the advantages of light scattering coupled to SEC and field-flow fractionation for characterizing branching across a wide spectrum of polymers.

Poly(lactic-co-glycolic acid) (PLGA) is a copolymer based on glycolic acid and lactic acid. The two monomer units are linked together by ester linkages and form linear polyester chains. The _ nal product is biodegradable and biocompatible, and is approved by the U.S. Food and Drug Administration (FDA) for production of various therapeutic devices as well as for drug delivery applications. The properties of PLGA can be tuned by the ratio of the two monomers and by the overall molar mass distribution.

Quality and consistency in reagents is critical to successful drug discovery and development. When targeting a particular protein of interest, in vitro experiments should be performed with proteins of biological properties similar to those for in vivo tests. It is important that molecularity, purity, shape, and degree of heterogeneity remain the same when any alterations are made to the model protein or the formulation buffer. Multi-angle light scattering (MALS) combined with size-exclusion chromatography (SEC-MALS) is a very useful technique to monitor the solution properties of the protein as changes to reagents are made.

Poly(lactic-co-glycolic acid) (PLGA) is a copolymer based on glycolic acid and lactic acid. The two monomer units are linked together by ester linkages and form linear polyester chains. The _ nal product is biodegradable and biocompatible, and is approved by the U.S. Food and Drug Administration (FDA) for production of various therapeutic devices as well as for drug delivery applications. The properties of PLGA can be tuned by the ratio of the two monomers and by the overall molar mass distribution.