Daniela Held | Authors

Articles

Tips & Tricks GPC/SEC: UV–vis Detection

The most commonly applied detector in gel permeation chromatography/size-exclusion chromatography (GPC/SEC) is the differential refractive index detector, RI. How UV–vis detection, if applicable, adds true value to GPC/SEC applications is discussed in this instalment of Tips & Tricks.

Tips & Tricks GPC/SEC: How Do Calibration Curves Influence GPC/SEC Results?

Gel permeation chromatography/size-exclusion chromatography (GPC/SEC) is a relative method and that requires a calibration to obtain molar mass distribution information. The way that the type of calibrants and the quality of the calibration curve influences the results is discussed in this instalment of Tips & Tricks.

Tips & Tricks GPC/SEC: How to Treat Your RI Detector

The refractive index (RI) detector is the most common detector in gel permeation chromatography/size-exclusion chromatography (GPC/SEC). The advantage of this universal detector is that it detects everything; the disadvantage is that it detects everything. This instalment of “Tips & Tricks” offers some advice when working with RI detectors.

Tips & Tricks GPC/SEC: Inter-Detector Delay

Combinations of detectors are often used in gel permeation chromatography/size-exclusion chromatography (GPC/SEC) to measure absolute molar masses or chemical compositions as a function of elution volume. Such multidetector setups require the correction of the delay volume between the detectors for correct data processing. This instalment of Tips & Tricks explains more.