Maria T. Matyska, Ph.D. | Authors

Separation and retention of both polar and nonpolar compounds by the same stationary phase can be a useful approach for analyses of complex samples with a broad range of chemical properties. Typical stationary phases are designed for retention of either polar or nonpolar compounds so that multiple steps are required when designing a separation strategy. Hydride silica–based stationary phases are new materials with properties that allow for the simultaneous retention of both polar and nonpolar compounds over a range of aqueous–organic mobile phase compositions. Adjustment of the aqueous–organic ratio will determine whether polar or nonpolar compounds have greater retention.