How to Retain Polar and Nonpolar Compounds on the Same HPLC Stationary Phase with an Isocratic Mobile Phase
Separation and retention of both polar and nonpolar compounds by the same stationary phase can be a useful approach for analyses of complex samples with a broad range of chemical properties. Typical stationary phases are designed for retention of either polar or nonpolar compounds so that multiple steps are required when designing a separation strategy. Hydride silica–based stationary phases are new materials with properties that allow for the simultaneous retention of both polar and nonpolar compounds over a range of aqueous–organic mobile phase compositions. Adjustment of the aqueous–organic ratio will determine whether polar or nonpolar compounds have greater retention.