Michael McGinley | Authors


Evaluating Particle Size and Back Pressure in Peptide Mapping by UHPLC

Ultrahigh-pressure liquid chromatography (UHPLC) columns are used in peptide mapping to improve the resolution of highly complex peptide mixtures. It is commonly assumed that small particle columns increase performance, but this is not always the case. This study presents a comparison of peak count, column length, and resolution between core–shell and fully porous UHPLC columns.

Optimizing Gel Permeation Chromatography for Analyzing Polymers used as Drug Excipients

Although gel permeation chromatography (GPC) is not as widely used as other chromatography methods, it continues to be a useful technique for analyzing the size and solution characteristics of organic polymers. In this study, GPC was applied to analyze different polar polymers that are commonly used in drug excipients. As a result of their polarity, some specialized operating conditions were required. Optimizing the separation brought to light many of the common parameters involved in optimizing GPC separations, and those are discussed in detail here.