Regina Römling | Authors

Articles

Fast Antibody Titer Analysis with a New Protein A Column

Antibody related biopharmaceuticals represent one of the most dynamic segments of the pharmaceutical market. The analysis and monitoring of product titers in CHO cell cultures is one of the key tasks during development of new products. Cell line selection, optimization of expression rates, and process control need fast and efficient antibody quantification. Besides ELISA assays with known limitations with regard to reproducibility and precision, high performance liquid chromatography (HPLC) analysis using affinity chromatography is applied. This application note summarizes some performance data of a new Protein A affinity column for mAb titer analysis.