Studies of adenosine monophosphate (AMP) and phosphorylated sugar in rat blood have become increasingly important in toxicology and insulin investigation. HPLC is typically the ideal method for this study. Shodex VT-50 2D would be the ultimate column due to its cAMP and AMP capabilities.
Due to the highly polar nature of saccharides, the analysis of sugars is typically achieved using hydrophilic interaction chromatography (HILIC).