YMC America Inc | Authors


Investigation of Pore Size Particle Size and Column Geometry Effects on SEC Analysis of Monoclonal Antibodies

Smaller particle size stationary phases have seen increased use in the modern analytical laboratory due to their ability to exhibit greater resolution while enabling the use of smaller column geometries. Shorter column lengths and narrower bores allow for faster run times and lower flow rates, decreasing laboratory costs in terms of solvent consumption and man-hours needed to run the analyses. This application note examines the effects of smaller particle sizes in various column geometries on size-exclusion chromatography (SEC) methods and the method variables affecting the characterization of antibody products.

Choosing an Optimal Co-Solvent for Method Development in Supercritical Fluid Chromatography (SFC) for the Whelk-O®1, RegisPack®, and RegisCell® Chiral Stationary Phases

The majority of the samples in this study and other studies show that a majority of compounds can be separated by the use of the three most common alcohols: IPA, CH3OH, and ethanol. Solubility becomes a crucial factor in preparative chromatography. Combinations of solvents can be used to improve solubility when the chosen co-solvent has poor solubility of the compound in question.

Ruggedness of YMC Cyano-High Strength Stationary Phase Under Acidic and Basic Extremes

Silica-based cyano stationary phases have historically exhibited poor stability and short column lifetime at pH extremes. This application note details an investigation of the stability of YMC's Cyano-HG (high strength) 10 ?m silica-based stationary phase, which was performed at a customer's request.