Special Issues-11-01-2019

Special Issues

Vol 37, s11 Advances in Biopharmaceutical Analysis

November 01, 2019

Special Issues November 2019

37

11

Click the title above to open the Advances inBiopharmaceutical Analysis 2019 special issue in an interactive PDF format.

Advancing Biopharmaceutical Analysis

November 01, 2019

Articles

37

11

An introduction to this special issue by our guest editor

Monitoring of On-column Methionine Oxidation as Part of a System Suitability Test During UHPLC–MS/MS Peptide Mapping

November 01, 2019

Articles

37

11

Long-term column use can lead to on-column methionine oxidation during LC–MS/MS peptide mapping of antibody-based biotherapeutics. Following the approach described here minimizes the risk of measuring oxidative artifacts, and helps generate high quality data to provide reliable quantitative information about product-related heterogeneities.

Utilizing Multidimensional LC–MS for Hydroxyl Radical Footprinting Analysis

November 01, 2019

Articles

37

11

The potential of multidimensional online peptide mapping analysis as a strategy for improving a postlabeling workflow for protein–protein interactions is demonstrated using both hydroxy radical footprinting–mass spectrometry (HRF–MS) and LC–MS/MS.

Development of a Size-Exclusion Chromatography Method to Characterize a Multimeric PEG–Protein Conjugate

November 01, 2019

Articles

37

11

A simple and robust size-exclusion chromatography (SEC) method has been developed for characterizing a multimeric PEG–protein conjugate. A wide range of size variants of the conjugate, ranging from 50 kDa to >1000 kDa, can be resolved and quantitated.