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N-Glycan Separations: How Hydrophilic Interaction Liquid Chromatography (HILIC) Columns Affect Data Quality

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Event Overview

Glycosylation can be a critical quality attribute (CQA) or product quality attribute (PQA) of biotherapeutic proteins, as the structure of N-linked glycans can influence protein function. Analysis of released N-glycans labeled with a dye that facilitates hydrophilic interaction liquid chromatography (HILIC) with detection by fluorescence (FLD) and/or mass spectrometry (MS) is a commonly used approach. The selection of an appropriate HILIC column for these separations is critical to ensure that glycans in complex mixtures are well-separated with minimal coelutions. Robustness in peak shape, retention time, and overall column lifetime are also often cited as important factors in choosing a suitable column.
This webinar will: 
  • Present data on biotherapeutic molecules showing the N-glycan separation performance of Agilent’s latest developmental HILIC LC column
  • Provide comparisons of key N-glycan separations alongside other popular columns
  • Discuss key factors in column selection, such as selectivity, coelutions, peak shape, temperature stability, and longevity
  • Discuss how mobile phase composition can affect N-glycan charge group selectivity
  • Comparisons of MS and FLD response
Key Learning Objectives
  • Effects of column performance on N-glycan data analysis and data quality
  • Similarities and differences between HILIC LC columns from different manufacturers
  • Effects of mobile phase composition on N-glycan profile and separations
Who Should Attend
  • Biopharma analytical development scientists
  • Biopharma research and development scientists
Featured Speakers:

Tom Rice
Tom Rice
Product Manager, Bioconsumables
Agilent Technologies
Tom Rice is the Bioconsumables Product Manager at Agilent Technologies, coming to Agilent through the acquisition of ProZyme in 2018. Prior to joining Agilent, he held various sales and marketing roles within ProZyme, beginning in 2014. Much of Tom’s work both at ProZyme and Agilent has been focused on bringing innovative glycobiology solutions to biopharma customers. Tom is based in California and holds a BS from Sonoma State University.

Speaker’s Email: tom.rice@agilent.com

Randall Robinson
Randall Robinson
Research and Development Scientist
Agilent Technologies
Randall Robinson is a Research and Development Scientist in the chemistries and supplies division at Agilent. Prior to joining Agilent in 2021, Randall completed his PhD in food chemistry with a focus on developing analytical techniques to characterize bioactive oligosaccharides milk and dairy streams. Since joining Agilent, he has contributed to the design of numerous products utilized for N-glycan analysis, including the HILIC column described in this presentation.

Speaker’s Email: randall.robinson@agilent.com 
Speaker’s LinkedIn: www.linkedin.com/in/randall-c-robinson

For any technical question please contact Jordan Ramesh: jramesh@mjhlifesciences.com