HPLC Analysis of Oligonucleotides

Event Overview:

This session will provide an overview of the high-performance liquid chromatography (HPLC) analysis of oligonucleotides. The presentation will review the basics of nucleotides and how synthetic oligonucleotides are produced, introducing the audience to some of the language used in support of these molecules. Additionally, both anion exchange and ion-paired reversed phase analysis will be examined, moving on to an introduction to the application of mass spectrometry (MS) to oligonucleotides.

Topics Covered:

Biotechnology
Oligonucleotides overview
Therapeutic oligonucleotides
Oligonucleotide synthesis
Oligonucleotide chromatographic considerations
Anion exchange chromatography
Ion pair reversed phase chromatography
Mass spectrometry considerations
Ion pair-MS
Intact mass spectrum
IP-MS/MS

Featured Speaker:

Colin Towers

Senior Technical Support Chemist

Element

After completing a degree in pharmacy, Colin pursued a career in analytical chemistry. Following three years performing routine testing and three years instrument maintenance and calibration, he moved into a role as a method development and validation specialist in contract research. Gaining 11 years of experience in developing methods for HPLC, LC-MS, GC, GC-MS, and SPE in a high-throughput commercial environment, this was supplemented by three years lecturing in forensic analytical toxicology, as well as a further three years working as a community pharmacist. Colin has been with Element since 2011 as a technical specialist, consultant, and trainer.

For any technical questions please contact Jordan Ramesh: jramesh@mjhlifesciences.com

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HPLC Analysis of Oligonucleotides

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