Better Oligonucleotide Purifications Using an Analytical-Scale Model

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Event Overview:

Oligonucleotide purifications on ¾ or ½ cm size columns using Agilent analytical HPLC hardware lowers the cost of purification development and enables a vast array of options for research-scale production. Development of large-scale methods is accelerated by reducing the cost-per-iteration. If the goal is simply small scale production then benefits accrue from being able to utilize any resin available from the vast analytical separations world, including micron-sized beads.

Key Learning Objectives:

• Analytical-scale purifications requires much less in feed stock, resin, and mobile phase, greatly reducing costs compared to larger ‘traditional’ pilot-scale purifiers
• In a development context, more iterations with the same feed stock yields better data around parameter changes
• At this scale, pressure limits are much higher, enabling use of ~1 micron beads for simple production runs

Speaker:

Jeffrey Knight, Purification Process Development Specialist, Agilent Technologies

Time and Date: Tuesday, March 23, 2021 at 11am EDT | 8am PDT | 3pm GMT | 4pm CET

Sponsor: Agilent

Register Free: https://www.chromatographyonline.com/lcgc_w/purifications