Methods for Pharmaceutical Analytes and Oligonucleotides Using Elevated pH Conditions

John Yates’ lab is using MOBILion’s Parallel Accumulation Mobility Aligned Fragmentation (PAMAF) technology to enhance the DeGlyPHER workflow, which enables detailed, site-specific analysis of N-glycosylation in viral spike proteins such as SARS-CoV-2. This method combines sequential deglycosylation with high-sensitivity LC-MS to quantify glycan occupancy and processing, offering insights into the relationship between glycosylation and immune response. DeGlyPHER has been applied to over 200 vaccine candidates, including those for HIV, influenza, and Ebola, advancing understanding of glycoprotein-based immunogen design. This executive summary explains how DeGlyPHER is transforming vaccine development in real time, driving progress toward more effective and consistent immunization strategies, and delivering a more sensitive and high-throughput approach compared to traditional glycoproteomic methods.

Discover the supremacy of the bioinert coated hardware of YMC Accura BioPro IEX QF columns for reproducible analysis of oligonucleotides in ion-exchange chromatography.

This non-IP-RP method, using ammonium bicarbonate as mobile phase additive, is highly sensitive and suitable for the bioanalysis of therapeutic oligonucleotides.

This application with data from Roche Diagnostics confirms superior performance of bioinert-coated YMC Accura BioPro IEX SF columns for the IEX-MS analysis of mAbs.

Discover the supremacy of the bioinert coated hardware of YMC Accura BioPro IEX QF columns for reproducible analysis of oligonucleotides in ion-exchange chromatography.

This application note provides you an optimum RP method for the DAR determination of a site-specific ADC using a widepore YMC-Triart Bio C4 column.