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9 January 2014
In this Issue
Troubling Peaks: Why Do Some Peaks Front While Others Don’t?
Why do some peaks front and others don't in the same method? In this featured case, injections of the reference standard gave normal, nearly symmetrical peaks, but when the samples were injected, the peaks rose more slowly from the baseline than normal, resulting in a fronting peak, where the first half of the peak is broader than the second half. John Dolan discusses the reasons behind this and how to correct the problem. More...
FMS
Automated Solid Phase Extraction of Base, Neutral and Acid Semi Volatiles in Waste Water
Solid Phase Extraction has long been used for the analysis of semi-volatile organics in clean matrices. Due to the unique challenges inherent with waste water matrices adhered to LLE protocols. Advances in packing materials and the new automated FMS TurboTrace ABN SPE extraction system are now making extractions of difficult matrices commonplace for SPE. Register to attend a Free Webinar on January 29th 11:pm EST learn how improve the quality and throughput for your laboratories extractions. 
Thermo Fisher Sells Assets to Expedite Life Technologies Acquisition
Thermo Fisher Scientific (California, USA) has signed an agreement with GE Healthcare (St Giles, UK) to sell business assets for $1.06 billion. Thermo Fisher Scientific committed to sell its cell culture, gene modulation, and magnetic beads businesses in 2013 to expedite approval of its pending acquisition of Life Technologies by the European Commission. More...
Agilent Technologies
Advance Protein Characterization
Resolve and identify critical peptides in a digest with Agilent AdvanceBio Peptide Mapping columns, specially tested with a demanding peptide mix to ensure reliable peptide mapping performance. Explore AdvanceBio columns for LC/MS and LC/UV, and take your characterization further. Get tips and advice.
Fred Strohmeier Promoted to Agilent Senior VP and President of Life Sciences and Diagnostics Group
Agilent Technologies (California, USA) has announced the promotion of Fred Strohmeier, vice president and general manager of Agilent's Life Science Products and Solutions, to senior vice president, Agilent, and president of Agilent's Life Sciences and Diagnostics Group (LDG), effective immediately. More...
HTC 2014
Hyphenated Techniques in Chromatography (HTC-13) - January 28-31, 2014
The 13th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology will feature recent findings from academic and industrial experts on chromatographic and MS techniques covering fundamental aspects, instrumental developments and state-of-the-art applications. Learn More
Current features
A Rapid HPLC Method for Enabling PAT Application for Processing of GCSF
This instalment of "Biotechnology Today" focuses on developing a rapid method for reversed-phase high performance liquid chromatography (HPLC) analysis of granulocyte colony stimulating factor (GCSF) purity for process analytical technology (PAT) applications. It also highlights an approach that one could take to achieve faster HPLC analysis. More...
Diisobutyl Columns Reduce Solvent Consumption and Offer Rapid HPLC–MS Analysis of Plasma Oxycodone and its Metabolites
Oxycodone and its metabolites were analyzed by high performance liquid chromatography–mass spectrometry (HPLC–MS) using a diisobutyl stable bond column. This article discusses the results. More...
Multimedia
Improving GPC Reproducibility and Resolution in Polymer Characterization
Editors’ Series: Analytical Tools for the Characterization of Biopharmaceuticals: Part I: Chromatographic Methods
The CHROMacademy Essential Guide Webcast: HPLC Detectors – What, Where, When, and How
Need LC Troubleshooting Help?

CHROMacademy has developed the HPLC Troubleshooter with busy chromatographers in mind. Sponsored by Thermo Scientific, in 3 simple steps you will overcome your instrument, separation and quantitation issues. It’s completely free - so if you haven't tried our troubleshooter before you're in for a surprise! Click Here
The Column
The Global E-zine for Separation Scientists
The global edition of The Column is delivered twice monthly with a screen-optimized format designed to improve readability. Covering topical issues, application-based articles, market reports, tips and tricks, opinion pieces and interviews, The Column is a great information resource helping readers achieve better day-to-day working practices.
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