Liquid Chromatography

Retention and Selectivity of Stationary Phases Used in HILIC

By David S. Bell

Understanding the interactions that stationary-phase chemistries provide will help you choose the most appropriate blend of interactions to address a given separation challenge.

Large-Volume Injection LC–MS-MS Methods for Aqueous Samples and Organic Extracts

By B. McKay Allred, Matthew J. Perkins, Jennifer A. Field

Environmental sample analysis by large-volume injection (LVI) in combination with liquid chromatography–tandem mass spectrometry (LC–MS-MS) is described for polar and nonpolar analytes in both aqueous samples and organic extracts.

Seven Things to Avoid in the Liquid Chromatography Laboratory

By John W. Dolan

Have a problem with your LC system? Maybe applying a little "DDT (Don't Do That)" may help.

Gas Chromatography

Pragmatic Rules for GC Column Selection

By Tony Taylor

Choosing the correct column selectivity and dimensions is fundamental to successful gas chromatography method development.

GC Detectors: From Thermal Conductivity to Vacuum Ultraviolet Absorption

By Kevin A. Schug, Harold M. McNair

Some might consider gas chromatography (GC) a mature technique. However, several substantial advances in GC technology in the past few years have proven that there is still room for innovation.

Critical Evaluation of GC Methods

By Tony Taylor

How to spot weaknesses in methods before problems occur

Mass Spectrometry

Unraveling the Links Between Diet and Human Health Using LC–MS-MS

By LCGC Editors

We recently spoke to Gary Duncan and Wendy Russell of the Rowett Institute of Nutrition & Health in Aberdeen, Scotland, about the significance of phytochemical bioavailability to human health and the important role of liquid chromatography linked to tandem mass spectrometry (LC–MS-MS) in their research.

GC–MS Analysis of an Herbal Medicinal Remedy to Identify Potential Toxic Compounds

By Irene W. Kimaru, Hang P. Nguyen

In most countries, herbal medicinal products (HMPs) are introduced into the market without proper scientific evaluation or enforced safety and toxicological studies.

Simultaneous Determination of Methylxanthines and Cotinine in Human Plasma by Solid-Phase Extraction Followed by LC–MS-MS

By Rossana Bossi, Bodil Hammer Bech

A multiresidue method has been developed and validated for the analysis of methylxanthines (caffeine and its metabolites) and cotinine in human plasma.

Sample Preparation Techniques

Simplifying Liquid Extractions

By Erica Pike

The benefits of supported liquid extraction (SLE) in sample cleanup and, in particular, the use of a synthetic SLE sorbent are discussed.

Pyrolysis for the Preparation of Macromolecules and Analysis by Gas Chromatography

By Douglas E. Raynie

When pyrolyzed, macromolecules will decompose into smaller fragments that can have the appropriate volatility for gas chromatography (GC) separation and analysis.

Understanding and Improving Solid-Phase Extraction

By Douglas E. Raynie, Dawn Wallace Watson

In this installment of "Sample Prep Perspectives," we cover some of the basic scientific principles behind solid-phase extraction (SPE) to allow the correct mode of extraction to be selected through an understanding of how analytes interact with and are separated by the sorbent.

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The LCGC Blog: How to Get the Most Out of Your First Conference Experience

By Kevin A. Schug

In this blog installment, I would like to share a little advice with those of you who are attending your first major conference.

HPLC Column Transfer from Fully Porous to Core-Shell Particles in Three Simple Equations

By Tony Taylor

There are often times in my work when I need to 'mess about' with column dimensions and particle morphologies. For 'mess about' read improve or transfer.

Latest News

Joseph Jack Kirkland: LCGC’s 2015 Lifetime Achievement Award Winner

Joseph Jack Kirkland, considered by many to be one of fathers of high performance liquid chromatography (HPLC), is the winner of LCGC’s 2015 Lifetime Achievement Award.

Improving LC–MS–MS Determination of Trace Estrogens in Serum

Detecting estrogens at trace concentrations in biological fluids is key to understanding their role in the induction — and treatment — of disease. Researchers from the University of Texas Arlington, USA, have developed a method using restricted access-media (RAM) that enables the detection of trace levels of oestrogen's using LC–MS–MS without liquid–liquid extraction (LLE) or solid-phase extraction (SPE).

Investigating Insect Communication with HPLC-ELSD

Scientists have published a novel method using reversed-phase high performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) for the isolation and determination of nonvolatile methyl-branched hydrocarbons (MBCHs) from insect cuticles.

Supplements Industry Plans Further Testing of Products Branded as Fraudulent

In response to recent accusations levied by the New York State Attorney General’s office that herbal supplements sold by four major retailers contained little or no primary ingredient, the supplements industry is planning further testing of the affected products.

Analyzing Swimming Pool Water

Pharmaceutical and personal care products (PPCPs) entering water supplies have been widely studied, but there is little information on the activity of these compounds in swimming pool water. Researchers have applied a PPCP analysis method previously developed for the detection of 32 PPCPs in wastewater to survey indoor swimming pools in the USA.



Comparison of LC–MS and GC–MS Analysis of Pharmaceuticals and Personal Care Products in Surface Water and Treated Wastewaters

Tuesday, March 17, 2015 — 8:00 am PDT/ 11:00 am EDT/ 4:00 pm GMT/ 5:00 pm CET



Pesticide Residue Analysis Webinar Series: Tips and Tricks for the Whole Workflow

Tuesday, March 24 2015/ Wednesday, April 29 2015/ Wednesday, June 17 2015/ Wednesday, July 15, 2015



Use of Novel Zirconia-Based Cleanup Sorbents for the Analysis of Contaminants in Fatty Food Samples

Tuesday, March 31, 2015 — 8:00 am PDT/ 11:00 am EDT/ 4:00 pm GMT/ 5:00 pm CET



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Featured: Applications of Ion Chromatography

You know the classic uses of ion chromatography (IC), like measuring inorganic ions in drinking water. But do you know what else it can do?



My LC–MS isn’t behaving! Where do I start?

Instrument manufacturers try to convince us that mass spec is just another detector. Most of us who work with LC-MS know that’s simply not the case – they can be maintenance intensive, unforgiving and generate complex information. When they’re not working it can be difficult to work out exactly where the problem lies. Here’s some advice to point you in the right direction

HPLC Column Dead Volume

A question about column dead volume. If the hold-up volume (time) is the extra-column dead volume expressed in time or volume. What is the column dead volume and how can it be calculated?

Seven Must Know Points to Understand Atmospheric Pressure Chemical Ionization

CHROMacademy delivers key knowledge quickly in bite-size modules, and to prove this here is our seven point guide to understanding atmospheric pressure chemical ionization.

HPLC Extra Column Volumes

It is of particular importance to reduce extra column volumes when using small volume columns or UHPLC. However, where do these extra column volumes come from, how can they be minimized, and what effect do they have on chromatography?