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LCGC TV: 2D LC Methods for Analyzing Pharmaceutical Impurities: Two-dimensional liquid chromatography has moved from academia into industry. In this new video, Kelly Zhang of Genentech talks about the advantages gained by developing a two-dimensional LC method for analyzing impurities in pharmaceuticals, and gives practical examples.

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LCGC COLUMNISTS 2014

Column Watch: Ron Majors, established authority on new column technologies, keeps readers up-to-date with new sample preparation trends in all branches of chromatography and reviews developments. LATEST: Avoiding Reversed-Phase Chromatography Problems Through Informed Method Development Practices: Choosing the Stationary-Phase Chemistry


Perspectives in Modern HPLC: Michael W. Dong is a senior scientist in Small Molecule Drug Discovery at Genentech in South San Francisco, California. He is responsible for new technologies, automation, and supporting late-stage research projects in small molecule analytical chemistry and QC of small molecule pharmaceutical sciences. LATEST: Seven Common Faux Pas in Modern HPLC


MS — The Practical Art: Kate Yu brings her expertise in the field of mass spectrometry and hyphenated techniques to the pages of LCGC. In this column she examines the mass spectrometric side of coupled liquid and gas-phase systems. Troubleshooting-style articles provide readers with invaluable advice for getting the most from their mass spectrometers. LATEST: Radical Mass Spectrometry as a New Frontier for Bioanalysis


LC Troubleshooting: LC Troubleshooting sets about making HPLC methods easier to master. By covering the basics of liquid chromatography separations and instrumentation, John Dolan is able to highlight common problems and provide remedies for them. LATEST: Estimating Resolution for Marginally Separated Peaks


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Features
Avoiding Reversed-Phase Chromatography Problems Through Informed Method Development Practices: Choosing the Stationary-Phase Chemistry
Understanding the chemistry of embedded polar group and perfluorophenyl phases leads to better decisions during method developement
Maintaining Precision During Method Transfer
Transferring methods between laboratories can be extremely difficult. Mary Ellen McNally from DuPont Crop Protection gives her advice on best practices for maintaining precision during method transfer.
Fractal Chromatography: A New Phase in Separation Science?
The Column spoke to Tony Edge of Thermo Fisher Scientific about the development of a new type of stationary phase based on fractal-shaped particles.
Comparison of Enantiomeric Separations and Screening Protocols for Chiral Primary Amines by SFC and HPLC
Supercritical (subcritical) fluid chromatography (SFC) was evaluated as an alternative to HPLC for the enantiomeric separation of primary amines on a cyclofructan-based chiral stationary phase.
Quantifying Proteins by Mass Spectrometry
A review of the essential elements of MS approaches to protein quantification and a critical comparison of the available options

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