Incognito discusses how investigating negative dat...
Have a problem with your LC system? Maybe applying a little "DDT (Don't Do That)" may help.
Environmental sample analysis by large-volume injection (LVI) in combination with liquid chromatography–tandem mass spectrometry (LC–MS-MS) is described for polar and nonpolar analytes in both aqueous samples and organic extracts.
By LCGC Editors
LCGC recently spoke with the 2014 LCGC Emerging Leader in Chromatography award winner, Andre de Villiers, an Associate Professor of Science at Stellenbosch University in South Africa, about his current research analyzing natural products, his various interests and collaborations in analytical chemistry, and his role as a teacher.
Some might consider gas chromatography (GC) a mature technique. However, several substantial advances in GC technology in the past few years have proven that there is still room for innovation.
A new, single method to replace the two-method approach using EPA Methods TO-15 and TO-13A to analyze both volatile and semivolatile organic compounds in air
This article describes the development of a new data-independent acquisition (DIA) workflow for protein quantification that uses a mass spectrometer that combines three types of mass analyzers to achieve lower limits of detection (LOD), higher sensitivity, more accurate quantitative results, wider dynamic range, and better reproducibility than existing high-resolution accurate-mass (HRAM) tandem mass spectrometry (MS-MS) DIA workflows.
In most countries, herbal medicinal products (HMPs) are introduced into the market without proper scientific evaluation or enforced safety and toxicological studies.
Worldwide trends in illicit drug use and production have shifted toward an increase in synthetic analogues and the emergence of new variations in their manufacture.
When pyrolyzed, macromolecules will decompose into smaller fragments that can have the appropriate volatility for gas chromatography (GC) separation and analysis.
In this installment of "Sample Prep Perspectives," we cover some of the basic scientific principles behind solid-phase extraction (SPE) to allow the correct mode of extraction to be selected through an understanding of how analytes interact with and are separated by the sorbent.
Green analytical chemistry is a widely recognized concept that has led to the development of new analytical methods with reduced environmental impact and minimized analyst occupational exposure. This article presents the most recent progress in the development of greener sample preparation and chromatographic separation techniques.
By Tony Taylor
If you ‘do’ Gas Chromatography, then you will have undoubtedly conditioned a capillary GC column ready for use after a period of storage or before first use. I see a lot of bad practice when columns are conditioned and so I thought that, even though this subject may have been covered many times, I’d try to produce a definitive guide with enough background information to explain why and how capillary GC columns should be conditioned.
I have had enough conversations with experts in the field of high performance liquid chromatography (HPLC) stationary-phase supports to know that there is more to the increased efficiency provided by the use of superficially porous particles (SPP) compared to fully porous particles (FPP) than simply mass transfer effects. Yet, I would argue that this is still one of the biggest misconceptions propagated by some members of the chromatography community.
A group of scientists from the University of Parma in Italy, has performed capillary ultrahigh-performance liquid chromatography and electrospray ionization quadrupole orbitrap high-resolution mass spectrometry (UHPLC–ESI Q-orbitrap–HRMS) to characterize materials used in food packaging and that consequently come into contact with food, namely polycarbonate food-contact plastics.1
A new study published in the Journal of Chromatography A presents a sensitive method for the quantification of apidaecins, a type of antimicrobial peptide that play an important role the immune response of honey bees to pathogens. The method was developed using reversed-phase nanoliquid chromatography coupled with high resolution mass spectrometry (LC–MS) to determine apidaecins in hemolymph or body parts from individual honey bees.
A new study published in the journal Analytical Chemistry presents a drug metabolism strategy based on microsome mesoporous organosilica nanoreactors coupled with high performance liquid chromatography–mass spectrometry (HPLC–MS) to screen for potential drug toxicity.
A new study published in the Journal of Chromatography A presents metabolite profiling of tea (Camella sinensis) harvested from the Bulang Mountains in Yunnan, China, using multidimensional gas chromatography–mass spectrometry (GC×GC–MS) to determine the impact of seasonal changes.
Scientists at the Norwegian Defence Research Establishment (Kjeller, Norway) are working in collaboration with the University of Oslo (Oslo, Norway) to develop new methods to detect the illicit use of nerve agents. In a recent study published in the journal Analytical Chemistry, the team presented a novel method for the quantification of nerve agent metabolites in human serum and urine by combining salting-out assisted liquid–liquid extraction (SALLE) and on-line solid-phase extraction–liquid chromatography–tandem mass spectrometry (SPE–LC–MS–MS).
In this new e-book, leading experts present the latest analytical tools for characterizing biopharmaceuticals, including chromatographic methods, mass spectrometry methods, and focused methods for analyzing carbohydrates on therapeutic glycoproteins.
CHROMacademy delivers key knowledge quickly in bite-size modules, and to prove this here is our seven point guide to understanding atmospheric pressure chemical ionization.
It is of particular importance to reduce extra column volumes when using small volume columns or UHPLC. However, where do these extra column volumes come from, how can they be minimized, and what effect do they have on chromatography?