Despite the utility of high performance liquid chromatography (HPLC) simulators, we found that all the free and low-cost simulators were outdated or had extremely limited functionality, so we created one that addressed these shortcomings.
Limonin was separated from other components in citrus juice sample matrices by reversed-phase chromatography with ultraviolet detection.
This article describes sampling methodology for thermal desorption–gas chromatography (TD–GC) that can extend the compatible analyte range of tests used to determine chemicals released from materials, as well as associated indoor air quality measurements.
The regulatory and practical issues that surround allergenic fragrance use within cosmetics and cleaning products are explored in this article.
The use of a mass spectrometer in quantitative analysis exploits its exquisite selectivity and sensitivity as a detector, allowing a signal to be ascribed to a particular chemical entity with high certainty, even when present in a sample at a low concentration. There are, however, some special considerations that are necessary when a mass spectrometer is used as a quantitative tool.
By LCGC Editors
We recently spoke to Gary Duncan and Wendy Russell of the Rowett Institute of Nutrition & Health in Aberdeen, Scotland, about the significance of phytochemical bioavailability to human health and the important role of liquid chromatography linked to tandem mass spectrometry (LC–MS-MS) in their research.
In most countries, herbal medicinal products (HMPs) are introduced into the market without proper scientific evaluation or enforced safety and toxicological studies.
When pyrolyzed, macromolecules will decompose into smaller fragments that can have the appropriate volatility for gas chromatography (GC) separation and analysis.
In this installment of "Sample Prep Perspectives," we cover some of the basic scientific principles behind solid-phase extraction (SPE) to allow the correct mode of extraction to be selected through an understanding of how analytes interact with and are separated by the sorbent.
By Tony Taylor
There are many aspects of analytical science which abound with myth and legend – but gas chromatography – mass spectrometry (GC-MS), and more specifically the electron ionization (EI) process, stands out as the technique which has given rise to the largest number of ‘urban myths’ and misunderstandings.
In this blog installment, I would like to share a little advice with those of you who are attending your first major conference.
The United Stated Pharmacopeial Convention (USP) has named Jaap Venema as the Chief Science Officer, beginning on April 15, and Chair of USP’s standards-setting body, the Council of Experts, at the beginning of USP’s next five-year cycle on July 1, 2015.
Caroline West, the winner of the 2015 LCGC Emerging Leader in Chromatography award, has a diverse set of scientific interests — in high-performance liquid chromatography (HPLC), supercritical fluid chromatography (SFC), hydrophilic-interaction chromatography (HILIC), and enantioselective separations — but all primarily focused on the fundamentals of chromatographic selectivity.
Researchers from the California Animal Health and Food Safety Laboratory at the University of California Davis in the USA have developed an ultrahigh-performance liquid chromatography–mass spectrometry (UHPLC–MS) method for the detection of desmethylbromethalin (DMB) residues in animal tissues.
Paralytic shellfish toxins (PSTs) are naturally occurring toxins produced by some species of microscopic algae that can accumulate in filter feeding shellfish. These toxins are a threat to shellfish aquaculture and pose a serious hazard to public health when ingested. A group of scientists has developed a method using hydrophilic interaction liquid chromatography coupled to ultrahigh?performance LC tandem mass spectrometry (HILIC UHPLC–MS–MS) to determine PSTs in a variety of shellfish species as part of the Safe New Zealand Seafood Research programme.
Fish from the Baltic Sea are a major source of lipophilic environmental pollutants for consumers in Finland. Surrounded by land, the Baltic Sea is one of the most threatened marine environments, making fish from the Baltic Sea a major source of lipophilic environmental pollutants including polybrominated diphenyl ethers (PBDEs).
Thursday, April 2, 2015 — 8:00 am PDT/ 11:00 am EDT/ 4:00 pm BST/ 5:00 pm CEST
Tuesday, April 7, 2015 — 8:00 am PDT / 11:00 am EDT / 4:00 pm BST / 5:00 pm CEST
PART II: Wednesday, April 29, 2015 — Workflow Guide for the use of LC-MS — 8:00am PDT/ 11:00am EDT/ 4:00pm BST/ 5:00pm CEST
The Diversity of UV/Vis/NIR Techniques for Nanomaterial Characterization: How to use transmission, scatter transmission, diffuse reflectance, and angular resolved scattering measurements to characterize nano structures
Wednesday, April 29, 2015 —8:00 am PDT/ 11:00 am EDT/ 4:00 pm BST/ 5:00 pm CEST
Thursday, April 30, 2015 — 9:00 am EDT/ 2:00 pm BST/ 3:00 pm CEST
Given the complexity of biological systems, studying the metabolome is a challenging undertaking. Prof. Oliver Fiehn, PhD, Director of the NIH West Coast Metabolomics Center, University of California, Davis, explains steps to follow in the metabolomic workflow and addresses common metabolomic questions
HPLC troubleshooting guide to cycling baselines and pressure fluctuations from LCGC's ChromAcademy.
Instrument manufacturers try to convince us that mass spec is just another detector. Most of us who work with LC-MS know that’s simply not the case – they can be maintenance intensive, unforgiving and generate complex information. When they’re not working it can be difficult to work out exactly where the problem lies. Here’s some advice to point you in the right direction
A question about column dead volume. If the hold-up volume (time) is the extra-column dead volume expressed in time or volume. What is the column dead volume and how can it be calculated?
CHROMacademy delivers key knowledge quickly in bite-size modules, and to prove this here is our seven point guide to understanding atmospheric pressure chemical ionization.
It is of particular importance to reduce extra column volumes when using small volume columns or UHPLC. However, where do these extra column volumes come from, how can they be minimized, and what effect do they have on chromatography?