Liquid Chromatography

Large-Volume Injection LC–MS-MS Methods for Aqueous Samples and Organic Extracts

By Jennifer A. Field, Matthew J. Perkins, B. McKay Allred

Environmental sample analysis by large-volume injection (LVI) in combination with liquid chromatography–tandem mass spectrometry (LC–MS-MS) is described for polar and nonpolar analytes in both aqueous samples and organic extracts.

Seven Things to Avoid in the Liquid Chromatography Laboratory

By John W. Dolan

Have a problem with your LC system? Maybe applying a little "DDT (Don't Do That)" may help.

The 2014 Emerging Leader in Chromatography: Andre de Villiers

By LCGC Editors

LCGC recently spoke with the 2014 LCGC Emerging Leader in Chromatography award winner, Andre de Villiers, an Associate Professor of Science at Stellenbosch University in South Africa, about his current research analyzing natural products, his various interests and collaborations in analytical chemistry, and his role as a teacher.

Gas Chromatography

GC Detectors: From Thermal Conductivity to Vacuum Ultraviolet Absorption

By Kevin A. Schug, Harold M. McNair

Some might consider gas chromatography (GC) a mature technique. However, several substantial advances in GC technology in the past few years have proven that there is still room for innovation.

Critical Evaluation of GC Methods

By Tony Taylor

How to spot weaknesses in methods before problems occur

Electronic Control of Carrier Gas Pressure, Flow, and Velocity

By John V. Hinshaw

How does a GC system set and control gas pressures, flows, and carrier gas velocities electronically?

Mass Spectrometry

Large-Scale Targeted Protein Quantification Using Wide Selected-Ion Monitoring Data-Independent Acquisition

By Andreas F.R. Hühmer, Sonia Ting, Reiko Kiyonami, Vlad Zabrouskov, Michael Senko, Jarrett Egertson, Michael MacCoss

This article describes the development of a new data-independent acquisition (DIA) workflow for protein quantification that uses a mass spectrometer that combines three types of mass analyzers to achieve lower limits of detection (LOD), higher sensitivity, more accurate quantitative results, wider dynamic range, and better reproducibility than existing high-resolution accurate-mass (HRAM) tandem mass spectrometry (MS-MS) DIA workflows.

GC–MS Analysis of an Herbal Medicinal Remedy to Identify Potential Toxic Compounds

By Hang P. Nguyen, Irene W. Kimaru

In most countries, herbal medicinal products (HMPs) are introduced into the market without proper scientific evaluation or enforced safety and toxicological studies.

Application of Ambient Sampling Portable Mass Spectrometry Toward On-Site Screening of Clandestine Drug Operations

By Seth E. Hall, Christopher C. Mulligan

Worldwide trends in illicit drug use and production have shifted toward an increase in synthetic analogues and the emergence of new variations in their manufacture.

Sample Preparation Techniques

Pyrolysis for the Preparation of Macromolecules and Analysis by Gas Chromatography

By Douglas E. Raynie

When pyrolyzed, macromolecules will decompose into smaller fragments that can have the appropriate volatility for gas chromatography (GC) separation and analysis.

Understanding and Improving Solid-Phase Extraction

By Douglas E. Raynie, Dawn Wallace Watson

In this installment of "Sample Prep Perspectives," we cover some of the basic scientific principles behind solid-phase extraction (SPE) to allow the correct mode of extraction to be selected through an understanding of how analytes interact with and are separated by the sorbent.

Developments in Green Chromatography

By Jacek Namiesnik, Marek Tobiszewski

Green analytical chemistry is a widely recognized concept that has led to the development of new analytical methods with reduced environmental impact and minimized analyst occupational exposure. This article presents the most recent progress in the development of greener sample preparation and chromatographic separation techniques.

Partner Organizations


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GC Column Conditioning Stop Wasting Time and Money!

By Tony Taylor

If you ‘do’ Gas Chromatography, then you will have undoubtedly conditioned a capillary GC column ready for use after a period of storage or before first use. I see a lot of bad practice when columns are conditioned and so I thought that, even though this subject may have been covered many times, I’d try to produce a definitive guide with enough background information to explain why and how capillary GC columns should be conditioned.

Insights on Increased Efficiency for Superficially Porous Particles Among Other Things

By Kevin A. Schug

I have had enough conversations with experts in the field of high performance liquid chromatography (HPLC) stationary-phase supports to know that there is more to the increased efficiency provided by the use of superficially porous particles (SPP) compared to fully porous particles (FPP) than simply mass transfer effects. Yet, I would argue that this is still one of the biggest misconceptions propagated by some members of the chromatography community.

Latest News

Characterizing Food Packaging Materials

A group of scientists from the University of Parma in Italy, has performed capillary ultrahigh-performance liquid chromatography and electrospray ionization quadrupole orbitrap high-resolution mass spectrometry (UHPLC–ESI Q-orbitrap–HRMS) to characterize materials used in food packaging and that consequently come into contact with food, namely polycarbonate food-contact plastics.1

Metabolic Profiling of Tea

A new study published in the Journal of Chromatography A presents metabolite profiling of tea (Camella sinensis) harvested from the Bulang Mountains in Yunnan, China, using multidimensional gas chromatography–mass spectrometry (GC×GC–MS) to determine the impact of seasonal changes.

Detecting Nerve Agent Exposure

Scientists at the Norwegian Defence Research Establishment (Kjeller, Norway) are working in collaboration with the University of Oslo (Oslo, Norway) to develop new methods to detect the illicit use of nerve agents. In a recent study published in the journal Analytical Chemistry, the team presented a novel method for the quantification of nerve agent metabolites in human serum and urine by combining salting-out assisted liquid–liquid extraction (SALLE) and on-line solid-phase extraction–liquid chromatography–tandem mass spectrometry (SPE–LC–MS–MS).

Speeding Up Drug Metabolism Screening

A new study published in the journal Analytical Chemistry presents a drug metabolism strategy based on microsome mesoporous organosilica nanoreactors coupled with high performance liquid chromatography–mass spectrometry (HPLC–MS) to screen for potential drug toxicity.


Make Your Lab Productivity Soar With the Right Chromatography Data System

Thursday, January 27, 2015 —8:00 am PST/ 10:00 am CST/ 11:00 am EST


Characterization of Functionalized Nanoparticles Using Ambient Ionization Mass Spectrometry

Wednesday, January 28, 2015 —8:00 am PST/ 11:00 am EST/ 4 pm GMT/ 5 pm CET


Editors' Series: Applications of Native MS and High Resolution Mass Spectrometry for the Characterization of Biotherapeutic Molecules

Thursday, January 29, 2015 —8:00 am PST/ 10:00 am CST/ 11:00 am EST


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LCGC eBooks

Featured: Critical Choices in HPLC

In chromatography, the better your understanding of the fundamental mechanisms, the easier it will be for you to develop successful methods. This new e-book provides a guide to essential information in HPLC.



Seven Must Know Points to Understand Atmospheric Pressure Chemical Ionization

CHROMacademy delivers key knowledge quickly in bite-size modules, and to prove this here is our seven point guide to understanding atmospheric pressure chemical ionization.

HPLC Extra Column Volumes

It is of particular importance to reduce extra column volumes when using small volume columns or UHPLC. However, where do these extra column volumes come from, how can they be minimized, and what effect do they have on chromatography?