Featured LCGC Interviews

Solid-Phase Microextraction in Clinical Diagnostics

Researchers at the University of Waterloo in Canada are collaborating with clinicians at Toronto General Hospital to develop preclinical and clinical applications of solid-phase microextraction (SPME). Bethany Degg of The Column spoke to Barbara Bojko from the team to find out more.

Liquid Chromatography

Gradient HPLC—One Invaluable Equation

By Tony Taylor

Here, we concentrate on one particularly useful equation that allows us to make changes to an analytical system to improve throughput or efficiency, while retaining the selectivity of the original method.

Distorted Peaks – A Case Study

By John W. Dolan

We consider the potential causes of peak distortion for the first two peaks in the chromatogram.

Questions of Quality: Where Can I Draw The Line?

By R.D. McDowall

A question that keeps raising its head when working in a regulated laboratory is can chromatographers integrate peaks manually? If they can, when can they do it? Also if they can manually integrate, when should they not do it?

Gas Chromatography

A Decade of Quantitating Cyanide in Aqueous and Blood Matrices Using Automated Cryotrapping Isotopic Dilution Static Headspace GC–MS

By Paul R. Loconto

Some insights into the principles and practice of implementing and improving on methods to quantitate cyanide at trace concentration levels in human blood.

Flame Ionization: GC's Workhorse Detector

By John V. Hinshaw

FID continues to play a crucial role for all types of gas chromatography, so it is important to understand how to use it effectively.

Separating and Identifying Trace-Level Chemicals in Wine by Headspace SPME with GC×GC–TOF MS

By Steve Smith, Laura McGregor, David Barden

Solid-phase microextraction (SPME) coupled with comprehensive two-dimensional gas chromatography and time-of-flight mass spectrometry (GC×GC–TOF-MS) can be used to detect trace-level fungicides and compounds responsible for undesirable attributes known as “organoleptic faults” in wine. This article explains more.

Mass Spectrometry

GC–MS Analysis of Aroma Compounds in Edible Oils by Direct Thermal Desorption

By Alexander Hasselbarth, Oliver Lerch

Determining aroma compounds and off-flavours in edible oils by gas chromatography coupled to mass spectrometry (GC–MS) is routine in many food-producing companies; however, the oily matrix needs to be kept out of the analytical instrument to avoid impacting analytical stability. The following article describes a simple, yet efficient, way of eliminating the matrix while determining the flavour compounds in a sensitive and selective manner.

Single-Cell MS and High-Spatial-Resolution MS Imaging Under Ambient Conditions Using a Novel Sampling Device

By Ning Pan, Wei Rao, Zhibo Yang

We have developed a miniaturized, multifunctional device, called the "Single-Probe," that is capable of probing small targets and of sampling and ionizing molecular species.

Nonconventional Alternatives to LC–MS

By Sarah Trimpin, Shubhashis Chakrabarty, Beixi Wang, Khoa Hoang, Corinne A. Lutomski, Ellen D. Inutan, Charles N. McEwen

An important attribute of a novel ionization process for use in mass spectrometry (MS) is its simplicity and flexibility to be hyphenated to conventional liquid-based separation methods.

Sample Preparation Techniques

Solid-Phase Microextraction in Clinical Diagnostics

By Bethany Degg

Researchers at the University of Waterloo in Canada are collaborating with clinicians at Toronto General Hospital to develop preclinical and clinical applications of solid-phase microextraction (SPME). Bethany Degg of The Column spoke to Barbara Bojko from the team to find out more.

Advances in Sample Preparation: Removing Phospholipids from Biological Samples

By Jonathan P. Danaceau, Xin Zhang, Pamela Iraneta, Michael Young, Kimvan Tran, Kim Haynes, Erin Chambers

Solid-phase extraction (SPE) has long been used for sample preparation — and is often chosen over liquid–liquid extraction (LLE) — because it can efficiently clean up and concentrate analytes of interest without the use of the more toxic organic solvents. This article describes the properties and use of a new SPE sorbent that removes phospholipids from biological samples, and illustrates the benefits of this approach with practical examples.

Aiding the Development of Extraction Procedures with Response Surface Methodology

By John Kiratu, Douglas E. Raynie

With response surface methodology, one can screen and optimize several extraction methods simultaneously. Here’s how.

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Paired Ion Electrospray Ionization for Trace Anion Analysis

By Kevin A. Schug

Using ESI-MS to perform quantitative binding analyses and determine association constants depends on the ability of the ionization process to preserve the system equilibrium.

Eight Steps to Better Results from Solid-Phase Extraction

By Tony Taylor

If you use SPE in your work, then most likely it’s very important to the success of your applications, and its proper implementation will be key to the performance of your analyses. In an effort to help you develop a better understanding of the technique, LCGC blogger Tony Taylor offers eight steps to solid-phase extraction success.

Latest News

Agilent Technologies Presents Thought Leader Award to Dr Lawrence Lesko

Agilent Technologies Inc. has announced that Dr. Lawrence J. Lesko from the University of Florida’s College of Pharmacy in Orlando, USA, has received an Agilent Thought Leader Award for his research into preclinical toxicological assessments of new medicines.

Koen Sandra Joins LCGC’s Editorial Advisory Board

LCGC is pleased to announce the addition of Koen Sandra to its editorial advisory board.

Diagnosing Diabetes Using Amino Acid Profiling of Hair

Profiling amino acids in scalp hair could aid the diagnosis of type 2 diabetes, according to a new study. Researchers performed gas chromatography–mass spectrometry (GC–MS) on hair samples taken from diagnosed patients and healthy patients to show that type 2 diabetes alters the amino acid composition of hair’s main building block, keratin, and that this biomarker could aid diagnosis in the clinic.

Improving Sanitation with the Help of GC–MS

As part of a wider project funded by the Bill & Melinda Gates Foundation to develop sustainable next-generation toilets for lower-income countries (the “Reinvent the Toilet Challenge”), researchers have applied gas chromatography–mass spectrometry (GC–MS) to profile the malodour compounds in the headspace of latrines in Africa and India.

David S. Bell Joins LCGC’s Editorial Advisory Board

LCGC Magazine is pleased to announce the addition of David S. Bell to its editorial advisory board.


HPLC Troubleshooting: Tips and Tricks

Wednesday, August 5, 2015 — 2 pm - 3 pm EDT 


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LCGC eBooks

Advancing Biopharma Analysis with Light-Scattering Detection

To characterize biopharmaceuticals, particularly monoclonal antibodies and antibody–drug conjugates (ADCs), you need a complete toolbox of powerful tools. You are probably familiar with LC–MS methods. But have you seen what light-scattering detection can do?

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Fundamentals of GC–MS Ionisation Techniques

Understanding how an instrument works is great, but when do you have time to read a textbook, or go to a course? Let CHROMacademy help. Dip in and out of concise modules and take 5 minutes to learn a little about the fundamentals of your analytical technique. Why not delve into GC-MS ionisation techniques right now?

Helium to Hydrogen — A Change Would Do You Good

Change is always a scary concept but let us dispel some of the fear associated with changing your helium carrier gas to hydrogen by answering some of the most common questions posed to us.

Reversed-Phase HPLC for the Analysis of Biomolecules

The following article from LCGC's ChromAcademy introduces the fundamentals of biopharmaceutical analysis and cover the use of reversed-phase HPLC in the analysis of biomolecules.

HPLC Troubleshooting Guide to Cycling Baselines and Pressure Fluctuations

HPLC troubleshooting guide to cycling baselines and pressure fluctuations from LCGC's ChromAcademy.

My LC–MS isn’t behaving! Where do I start?

Instrument manufacturers try to convince us that mass spec is just another detector. Most of us who work with LC-MS know that’s simply not the case – they can be maintenance intensive, unforgiving and generate complex information. When they’re not working it can be difficult to work out exactly where the problem lies. Here’s some advice to point you in the right direction