Liquid Chromatography

Retention and Selectivity of Stationary Phases Used in HILIC

By David S. Bell

Understanding the interactions that stationary-phase chemistries provide will help you choose the most appropriate blend of interactions to address a given separation challenge.

Large-Volume Injection LC–MS-MS Methods for Aqueous Samples and Organic Extracts

By B. McKay Allred, Matthew J. Perkins, Jennifer A. Field

Environmental sample analysis by large-volume injection (LVI) in combination with liquid chromatography–tandem mass spectrometry (LC–MS-MS) is described for polar and nonpolar analytes in both aqueous samples and organic extracts.

Seven Things to Avoid in the Liquid Chromatography Laboratory

By John W. Dolan

Have a problem with your LC system? Maybe applying a little "DDT (Don't Do That)" may help.

Gas Chromatography

The Smell of Success: Using GC–MS–MS for Low Level Allergenic Fragrance Detection

By Gordon van ‘t Slot, Joe Anacleto

The regulatory and practical issues that surround allergenic fragrance use within cosmetics and cleaning products are explored in this article.

Gas Chromatography Ovens

By John V. Hinshaw

The transit of peaks through a gas chromatography (GC) column depends strongly on the thermal profile they encounter on the way.

Pragmatic Rules for GC Column Selection

By Tony Taylor

Choosing the correct column selectivity and dimensions is fundamental to successful gas chromatography method development.

Mass Spectrometry

Quantifying Small Molecules by Mass Spectrometry

By P. Jane Gale, Alfred L. Yergey, Mark W. Duncan, Kate Yu

The use of a mass spectrometer in quantitative analysis exploits its exquisite selectivity and sensitivity as a detector, allowing a signal to be ascribed to a particular chemical entity with high certainty, even when present in a sample at a low concentration. There are, however, some special considerations that are necessary when a mass spectrometer is used as a quantitative tool.

Unraveling the Links Between Diet and Human Health Using LC–MS-MS

By LCGC Editors

We recently spoke to Gary Duncan and Wendy Russell of the Rowett Institute of Nutrition & Health in Aberdeen, Scotland, about the significance of phytochemical bioavailability to human health and the important role of liquid chromatography linked to tandem mass spectrometry (LC–MS-MS) in their research.

GC–MS Analysis of an Herbal Medicinal Remedy to Identify Potential Toxic Compounds

By Irene W. Kimaru, Hang P. Nguyen

In most countries, herbal medicinal products (HMPs) are introduced into the market without proper scientific evaluation or enforced safety and toxicological studies.

Sample Preparation Techniques

The Analysis of Chlorinated Dioxins Using Automated Pressurized Liquid Extraction, Multicolumn Cleanup, and Concentration

By Phillip Bassignani, FMS, Inc.

Traditional dioxin/difuran extraction procedures require 24 h soxlet extraction, and a wide array of manual sorbent cleanup procedures, each with their own evaporation step. This results in extensive manual prep hours, high levels of potential error, and increased potential for background or cross-contamination of samples. By utilizing the FMS total prep process, extraction and cleanup processes are automated into a high efficiency process capable of delivering same day analysis of these samples.

The Analysis of Chlorinated Dioxins Using Automated Pressurized Liquid Extraction, Multicolumn Cleanup, and Concentration

By Phillip Bassignani, FMS, Inc.

Traditional dioxin/difuran extraction procedures require 24 h soxlet extraction, and a wide array of manual sorbent cleanup procedures, each with their own evaporation step. This results in extensive manual prep hours, high levels of potential error, and increased potential for background or cross-contamination of samples. By utilizing the FMS total prep process, extraction and cleanup processes are automated into a high efficiency process capable of delivering same day analysis of these samples.

EPA Method 625 Determination of Bases, Neutrals, and Acids by SPE and GC–MS Detection

By Xiaoyan Wang, UCT Inc.

Historically EPA Method 625 (analysis of bases, neutrals and acids in municipal and industrial wastewater) was performed using a liquid-liquid extraction. Revision 625.1 has recently been published allowing the use of SPE as an alternative sample preparation technique. This application outlines the validation of this technique in various sample matrices using UCT’s specially designed sorbents. Excellent recoveries and low MDL’s were achieved.

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LCGC Blog

The LCGC Blog: Useful Tips for GC-MS

By Tony Taylor

There are many aspects of analytical science which abound with myth and legend – but gas chromatography – mass spectrometry (GC-MS), and more specifically the electron ionization (EI) process, stands out as the technique which has given rise to the largest number of ‘urban myths’ and misunderstandings.

The LCGC Blog: How to Get the Most Out of Your First Conference Experience

By Kevin A. Schug

In this blog installment, I would like to share a little advice with those of you who are attending your first major conference.

Latest News

Nominations Sought for 2017 Dal Nogare Award

The Chromatography Forum of the Delaware Valley is currently seeking nominations for the 2017 Dal Nogare Award.

Lung Cancer Breath Test in Clinical Trial

The University of Leicester and Glenfield Hospital in Leicester, UK, are starting a clinical trial into a gas chromatography–field asymmetric ion mobility spectrometry sensor (Owlstone Nanotech Ltd.) for the detection of early-stage lung cancer.

Thermo Fisher Scientific Buys Advanced Scientifics, Inc.

Thermo Fisher Scientific (Waltham, Massachusetts, USA) has acquired Advanced Scientifics, Inc. (ASI) (Millersburg, Pennsylvania, USA) for $300 million (US) in cash.

Determining Child Phthalate Exposure

Vinyl flooring in the home could be a key source of phthalates ingested and inhaled by children, according to a new study. The work was performed as part of an ongoing project by the Columbia Center for Children’s Environmental Health (CCCEH) at Columbia University in New York, USA, and followed a cohort of women and children living in New York city to investigate the impact of phthalate exposure on the health of children.

Joseph Jack Kirkland: LCGC’s 2015 Lifetime Achievement Award Winner

Joseph Jack Kirkland, considered by many to be one of fathers of high performance liquid chromatography (HPLC), is the winner of LCGC’s 2015 Lifetime Achievement Award.

Webcasts

 

Use of Novel Zirconia-Based Cleanup Sorbents for the Analysis of Contaminants in Fatty Food Samples

Tuesday, March 31, 2015 — 8:00 am PDT/ 11:00 am EDT/ 4:00 pm GMT/ 5:00 pm CEST

 

Simplifying Carbohydrate Testing in Food and Beverages to Meet Food Quality and Labeling Requirements Using Ion Chromatography and Pulsed Amperometric Detection

Thursday, April 2, 2015 — 8:00 am PDT/ 11:00 am EDT/ 4:00 pm BST/ 5:00 pm CEST

 

Trouble-Free HPLC Analysis of Creams and Lotions

Tuesday, April 7, 2015 — 8:00 am PDT / 11:00 am EDT / 4:00 pm BST / 5:00 pm CEST

 

More Upcoming & On Demand Webcasts>>

LCGC eBooks

 

Featured: Applications of Ion Chromatography

You know the classic uses of ion chromatography (IC), like measuring inorganic ions in drinking water. But do you know what else it can do?

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CHROMacademy

HPLC Troubleshooting Guide to Cycling Baselines and Pressure Fluctuations

HPLC troubleshooting guide to cycling baselines and pressure fluctuations from LCGC's ChromAcademy.

My LC–MS isn’t behaving! Where do I start?

Instrument manufacturers try to convince us that mass spec is just another detector. Most of us who work with LC-MS know that’s simply not the case – they can be maintenance intensive, unforgiving and generate complex information. When they’re not working it can be difficult to work out exactly where the problem lies. Here’s some advice to point you in the right direction

HPLC Column Dead Volume

A question about column dead volume. If the hold-up volume (time) is the extra-column dead volume expressed in time or volume. What is the column dead volume and how can it be calculated?

Seven Must Know Points to Understand Atmospheric Pressure Chemical Ionization

CHROMacademy delivers key knowledge quickly in bite-size modules, and to prove this here is our seven point guide to understanding atmospheric pressure chemical ionization.

HPLC Extra Column Volumes

It is of particular importance to reduce extra column volumes when using small volume columns or UHPLC. However, where do these extra column volumes come from, how can they be minimized, and what effect do they have on chromatography?