You may have an excellent educational background, and perhaps some very good experience, too. But are you prepared to get—and keep—your next job in analytical chemistry? This collection of short articles from LCGC’s digital magazine, The Column, offers essential advice for separation scientists who aspire to get a new job and succeed once there.
A balanced diet is one step closer to a healthy lifestyle. Samantha Duong from the Australian Government’s National Measurement Institute (NMI) has used gas chromatography with flame ionization detection (GC–FID) to measure phytosterols in fortified food. She recently spoke to us about this research.
In recent years, liquid chromatography–tandem mass spectrometry (LC–MS-MS) methods have been increasingly adopted as a replacement for ligand-binding assays to monitor the fate of drugs in vivo. Such bioanalysis of protein-based pharmaceuticals (biopharmaceuticals) is more challenging, however, than it is for small-molecule drugs. Rainer Bischoff of the University of Groningen in the Netherlands has been exploring approaches to overcome these challenges. He recently spoke to us about this work.
What are the principal hazards and safety issues surrounding high-pressure gas cylinders?
For testing various attributes of proteins, CE–MS is superior to LC–MS. And despite common fears, it is a robust tool. But adapting an LC–MS method to CE–MS requires careful optimization.
In clinical and forensic/toxicology laboratories, urine is a preferred matrix from which to quantify drug concentrations because it yields accurate results and allows for noninvasive collection methods. Prior to excretion, drug metabolites in the body undergo a glucuronidation reaction, resulting in a glucuronide bond that must be cleaved before mass spectrometry (MS) analysis by a β-glucuronidase enzyme hydrolysis. Many laboratories employ a “dilute-and-shoot” method after hydrolysis to decrease residual protein or enzyme concentration, but this method negatively affects column lifetime and reduces the sensitivity of analyte detection. By using a β-glucuronidase removal approach, analysts are able to see an increase in sensitivity and a reduction in MS instrument maintenance.
Many of this year’s new products fit into recently identified trends, but one major driver of advances was not in our forecasts.
By Tony Taylor
I started my career working in a Quality Control laboratory. I'm familiar with the constraints that must be imposed to ensure consistent global standards and the pace at which change can be implemented without loss of control either within a business or across an industry. OK—scene set.
I am quite excited about some recent work we and others have performed related to fuels analysis using gas chromatography and vacuum ultraviolet spectroscopy (GC–VUV).
A group of researchers have characterized carotenoid and chlorophyll in different sweet bell peppers (Capsicum annuum L.) using offline multidimensional supercritical fluid chromatography (SFC) combined with reversed-phase liquid chromatography (LC) for the first time.
Phenomenex (Torrance, California, USA) has announced that it will be acquired by Danaher Corporation (Washington, D.C., USA), a Fortune 150 science and technology company.
Friday, October 7th, 2016
8 am PDT / 11 am EDT / 4 pm BST / 5 pm CEST / 10 am CDT
Wednesday, October 12th, 2016
8 am PDT / 11 am EDT / 4 pm BST / 5 pm CEST
The medical use of cannabis is increasing. As a result, there is a pressing need to establish and disseminate rigorous methods for the characterization and routine testing of cannabis. Fortunately, experienced analytical chemists are bringing their expertise to the field. In this new e-book, developed in collaboration with the Cannabis Science Conference, leading experts share their perspectives on the current state of cannabis analysis, explain their contributions to advance it, and outline what is still needed.
Whether your GC instrument has only been shut down for a couple of days or has been gathering dust in a corner for a year, follow CHROMacademy's quick checklist to increase performance and reliability.
This course has been designed for anyone who is either new to LC–MS, or who wishes to expand their current knowledge and understanding about the technique as a whole.