Fundamental Questions

As you hurry through your daily work in the analytical laboratory, are you failing to ask yourself some fundamental questions about the methods you use and the results you produce?


This collection of short articles from LCGC’s digital magazine, The Column, raises four key questions you should ask. And if the answers aren’t what you hoped, we offer advice for addressing the gaps.


Read more.

Liquid Chromatography

How to Tackle an Unknown: Notes from the Fourth Method Development Olympics at CoSMoS

By Michael P. Balogh

How would you analyze a bag of gummy bears that showed up on your laboratory bench? This was the challenge taken on by teams of analysts in advance of the Conference on Small Molecule Science (CoSMoS) that was held in August 2015 in San Diego, California. This article shares insights from how the finalists approached the question.

Trends and Challenges for Bioanalysis and Characterization of Small and Large Molecule Drugs

By Suma Ramagiri

Structural, bioanalytical, characterization, and quality control studies are critical for successful drug development. These studies must be as accurate, sensitive, and selective as possible, and liquid chromatography coupled to tandem mass spectrometry (LC–MS–MS) has been the technique of choice for many areas of small molecule analysis for the past 30 years. During that time, rapid improvements in analytical technologies have supported the development of more sensitive and robust methods. However, the pharma and biopharma industry continues to need more powerful instruments and more diverse methods, particularly as therapeutics have expanded to include large molecules. This work follows on from an earlier article that explored the limitations of LC–MS–MS for bioanalysis of biologics. This article considers some of the current issues for analysis of small and large molecules, and emerging trends in method development.

Generic UHPLC Method for the Simultaneous Analysis of Compounds with a Wide Range of Polarities

By Glen Loos, Monika Dittmann, Konstantin Choikhet, Gert Desmet, Deirdre Cabooter

Appropriate analytical methods are required to evaluate the presence, metabolism, degradation, and removal of specific compounds in complex mixtures. There is an increasing demand to analyze samples with a wide range of polarities in a variety of applications, including environmental analysis, biomarker discovery, and proteomics. Multiple analyses on complementary columns are often needed to cover the separation of all compounds with a large difference in polarity. This article describes a generic method involving an ultrahigh-pressure liquid chromatography (UHPLC) system equipped with two external switching valves to connect hydrophilic interaction liquid chromatography (HILIC) and reversed-phase LC columns in series for the sequential analysis of polar and apolar compounds. The method was successfully applied to separate 32 pharmaceutical compounds with a wide range of polarities, which could be useful for analyzing pharmaceutical compounds in the environment.

Gas Chromatography

New Gas Chromatography Products for 2016

By John V. Hinshaw

The number and type of new product introductions this year are an indication of the continuing viability of gas chromatography.

How to Tackle an Unknown: Notes from the Fourth Method Development Olympics at CoSMoS

By Michael P. Balogh

How would you analyze a bag of gummy bears that showed up on your laboratory bench? This was the challenge taken on by teams of analysts in advance of the Conference on Small Molecule Science (CoSMoS) that was held in August 2015 in San Diego, California. This article shares insights from how the finalists approached the question.

The Importance of Specifications for Method Transfer

By Incognito

Incognito shares his thoughts on the importance of specifications for method transfer in gas chromatography (GC).

Mass Spectrometry

Review of New Spectroscopic Instrumentation 2016

By Howard Mark, Mike Bradley

Our annual review of products introduced at Pittcon or during the previous year, broken down by the following categories: accessories, atomic spectroscopy, components, imaging, mass spectrometry, mid-IR, NIR, NMR, Raman, software, UV-vis, and X-ray.

Desorption and Ionization Mass Spectrometry in Research Laboratories

By Giuseppe Astarita, Bindesh Shrestha

The three key applications for desorption and ionization techniques are rapid, in situ screening; direct analysis of extracted samples or planar chromatography spots; and scanning samples along x and y axes.

Extending the Hydrocarbon Range for the Analysis of Soil Gas Samples Using Automated Thermal Desorption Coupled with Gas Chromatography–Mass Spectrometry

By Lee Marotta, Stephen Varisco, Miles Snow, Tom Kwoka, Robert Thomas

When monitoring toxic gases emitted from the soil, it is often necessary to recover compounds with a boiling point above that of napthalene. The use of thermal desorption makes that possible.

Sample Preparation

New Sample Preparation Products and Accessories for 2016

By Douglas E. Raynie

Many of this year’s new products fit into recently identified trends, but one major driver of advances was not in our forecasts.

Trends in Sample Preparation

By Douglas E. Raynie

A comparison of the results from a new survey on sample preparation techniques to those of previous surveys, from 1991 to 2013, reveals some interesting trends.

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LCGC Blog

The LCGC Blog: Large Molecule Analysis for Small Molecule People

By Tony Taylor

In 2009 I wrote an article on the emerging field “Bio Chromatography,” which for a small molecule analyst such as myself, perfectly described the situation. I realize that the separation of biomolecules had been happening for many years, but the expansion and development of protein based therapeutics from that point onwards has seen an avalanche of developments in instrumentation, sample preparation, and column technologies the like of which I have not seen in my 30 year career.

The Middle Ground on Unconventional Oil and Gas Development (aka “Fracking”) Is a Lonely Place

By Kevin A. Schug

Compared to the magnitude of unconventional oil and gas development activity that has been performed by industry, the amount of research to assess its potential environmental impact has been miniscule. Can the process have a deleterious impact if not well managed? Can it be performed in a responsible manner?

LCGC TV

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LCGC Slideshows

Slideshow: Seven Common Faux Pas in Modern HPLC

Seven outdated traditional practices that should not be performed without considering alternative approaches that can improve results, provide lower operation costs, or give faster run times. Instead of working harder, analytical scientists should work smarter. Learn more by clicking through the slideshow.

Slideshow: The Top 10 HPLC and UHPLC Column Myths

Webster’s New Collegiate Dictionary defines a myth as “an ill-founded belief held uncritically, especially by an interested group.” Could that group be misinformed chromatographers?

Latest News

Derivatization-free GC Method to Analyze Oleic Acid Excipient

Researchers from the USA have developed a simple and derivatization-free gas chromatography method for the quantitative analysis of oleic acid and related fatty acids.

Deirdre Cabooter Joins LCGC’s Editorial Advisory Boards

LCGC magazine is pleased to announce the addition of Deirdre Cabooter to the editorial advisory boards of LCGC North America and LCGC Europe.

Debby Mangelings Joins LCGC’s Editorial Advisory Boards

LCGC magazine is pleased to announce the addition of Debby Mangelings to the editorial advisory boards of LCGC North America and LCGC Europe.

LCGC E-Books

Fundamental UHPLC Workflows for Biotherapeutic Characterization

The introduction of UHPLC revolutionized bio/pharma analytical laboratories and advances in technology promise more for the future. In this e-book, experts explain recent advances in UHPLC, and the basics of glycan analysis.

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CHROMacademy

The CHROMacademy Essential Guide Webcast: Techniques Employed in Biopharmaceutical Analysis – Part 1 Reversed Phase and HILIC

Biopharmaceuticals offer great new possibilities and potential in treating various life threatening diseases such as cancer and autoimmune diseases. However, their complexity, resulting from their heterogeneity, represents a great challenge to the analytical chemist and a suite of techniques is required for their characterization and analysis. Reversed phase chromatography, the mainstay of traditional small molecule analysis, is employed at multiple biopharmaceutical levels due to its versatility, inherent relative efficiency and ease of hyphenation to a mass spectrometer.

Peak Purity Algorithms using Diode Array Detectors

In an ideal chromatographic separation, all sample components would be isolated from each other and detected as fully resolved peaks. However, it is not uncommon to encounter some degree of overlap. In extreme cases, what appears to be a single peak contains in fact two or more coeluting components. This article discusses how to check the purity of such peaks in order to correctly interpret the results of the analysis.

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