As you hurry through your daily work in the analytical laboratory, are you failing to ask yourself some fundamental questions about the methods you use and the results you produce?
This collection of short articles from LCGC’s digital magazine, The Column, raises four key questions you should ask. And if the answers aren’t what you hoped, we offer advice for addressing the gaps.
Pavel Jandera discusses building his own LC instrument, the birth of modern HPLC, the importance of exploring (and understanding) earlier research papers, and current trends in contemporary chromatography. He also offers inspiring advice for young chromatographers.
This exploration of the fundamental interactions involved in PFP stationary phases will facilitate the choice of appropriate stationary phases and help analysts develop more robust and reliable methods.
The silica-based packing in reversed-phase columns is not inert. Here we consider what happens when the mobile phase pH is too high or too low.
By Jack Cochran
Jack Cochran’s new column “Practical GC” aims to provide readers with practical advice and new experimental evidence for how to get the best results from their gas chromatography (GC) systems. The first article in a series on split injection GC focuses on the advantages of using “shoot-and-dilute” GC.
Dead-volume effects can cause serious trouble for chromatographers. But if you understand what dead volume is and how it affects chromatographic results, you can take control of it.
By LCGC Editors
Imaging techniques using vibrational spectroscopy, MS, and atomic force microscopy have all been advancing and gaining momentum in recent years. There is great potential power in these imaging techniques, particularly in the biomedical field. Here, Thomas Bocklitz of the Friedrich-Schiller- University Jena discusses his work combining these techniques.
The power of nontargeted metabolite profiling is illustrated in a study focused on the determination of molecular markers in malting barley.
Is your swimming pool clean and safe? Recreational water illness, most commonly in the form of digestive tract illness or skin, ear, or respiratory infections, is often caused by water contamination. The authors present a robust method, using solid-phase extraction and high-resolution mass spectrometry, for monitoring swimming pool water.
A survey of LCGC readers on sample preparation techniques and methodology investigated trends in technologies being used, sample loads, sample sizes, automation, the use of SPE devices (cartridges, disks, plates, tips), SPE chemistries, selection criteria, and problems encountered.
By LCGC Editors
This first installment of the “Sample Prep Perspectives” column presents an overview of the primary manual and automated sample preparation techniques—methods that are much the same as those in use today.
As expected, the new products introduced in the past year in the area of chromatographic sample preparation, while somewhat limited, mirror the current development in the field.
Food analysis provides a rich sample matrix with many compounds of interest for analysis to contemplate—but one must always take care that the right tool is chosen for the desired task.
By Tony Taylor
Some modern HPLC systems resemble spacecraft in terms of their technology, designed as they are to operate to the highest efficiencies, compared to traditional systems. However, I still see countless examples where high efficiency columns are used on systems which are not matched and cannot support the highest efficiencies offered by the column.
Seven outdated traditional practices that should not be performed without considering alternative approaches that can improve results, provide lower operation costs, or give faster run times. Instead of working harder, analytical scientists should work smarter. Learn more by clicking through the slideshow.
Webster’s New Collegiate Dictionary defines a myth as “an ill-founded belief held uncritically, especially by an interested group.” Could that group be misinformed chromatographers?
Xiaoli Wang has been selected as the winner of the first 2016 Young Investigator Award from the Chinese American Chromatography Association (CACA).
Crawford Scientific (Strathaven, UK) has announced that it will become YMC Europe’s (Dinslaken, Germany) sole authorized distributor within the UK.
Sciex (Framingham, Massachusetts, USA) has announced a co‑marketing agreement with Pressure BioSciences, Inc. (South Easton, MA, USA), which aims to address the challenges arising in complex sample preparation.
Researchers from Imperial College London and the University of Aberdeen have identified disease markers for Human African Trypanosomiasis (HAT), commonly known as “sleeping sickness”. Using reversed-phase ultrahigh‑performance liquid chromatography coupled to mass spectrometry (UHPLC–MS) and proton nuclear magnetic resonance (1H NMR) spectroscopy researchers found 53 discriminatory metabolite markers.
A team of researchers based in France has examined the consequences of microwave and electron-beam treatments on food and its packaging using high performance liquid chromatography (HPLC) and gas chromatography (GC)
Tuesday, January 26, 2016 11am EDT/ 8am PDT/ 4pm BST/ 5pm CEST
Thursday, February 11th, 2016
8 am CST / 9 am EST / 2 pm GMT / 3 pm CET
Wednesday, February 24th, 2016
8 am PST / 10 am CST / 11am EST / 4 pm GMT / 5 pm CET
This e-book, based on a series of four web seminars, provides pesticide residue analysts with valuable information from Thermo Fisher Scientific about the development and optimization of methods and workflows for the analysis of pesticide residues in food.
If the hold-up volume (time) is the extra-column dead volume expressed in time or volume what is the column dead volume and how can it be calculated?
They say a picture paints a thousand words…This month I’ve taken inspiration from a recent webcast, presented at www.chromacademy.com, in which I presented real data from our work that represents some ‘classic’ GC problems.