Liquid Chromatography

An Open-Source Simulator for Exploring HPLC Theory

By Daniel Abate-Pella, Dwight R. Stoll, Peter W. Carr, Paul G. Boswell

Despite the utility of high performance liquid chromatography (HPLC) simulators, we found that all the free and low-cost simulators were outdated or had extremely limited functionality, so we created one that addressed these shortcomings.

Choosing the Right HPLC Stationary Phase

By Tony Taylor

There is a bewildering array of stationary-phase choices available for reversed-phase high performance liquid chromatography (HPLC), and even within each phase designation (such as "C18") the selectivity of each phase can vary widely.

LC–MS-Compatible Approaches for the Quantitation of Limonin in Citrus Juice

By Joshua E. Young, Joseph J. Pesek, Maria T. Matyska

Limonin was separated from other components in citrus juice sample matrices by reversed-phase chromatography with ultraviolet detection.

Gas Chromatography

The Smell of Success: Using GC–MS–MS for Low Level Allergenic Fragrance Detection

By Gordon van ‘t Slot, Joe Anacleto

The regulatory and practical issues that surround allergenic fragrance use within cosmetics and cleaning products are explored in this article.

Gas Chromatography Ovens

By John V. Hinshaw

The transit of peaks through a gas chromatography (GC) column depends strongly on the thermal profile they encounter on the way.

Pragmatic Rules for GC Column Selection

By Tony Taylor

Choosing the correct column selectivity and dimensions is fundamental to successful gas chromatography method development.

Mass Spectrometry

Quantifying Small Molecules by Mass Spectrometry

By P. Jane Gale, Alfred L. Yergey, Mark W. Duncan, Kate Yu

The use of a mass spectrometer in quantitative analysis exploits its exquisite selectivity and sensitivity as a detector, allowing a signal to be ascribed to a particular chemical entity with high certainty, even when present in a sample at a low concentration. There are, however, some special considerations that are necessary when a mass spectrometer is used as a quantitative tool.

Unraveling the Links Between Diet and Human Health Using LC–MS-MS

By LCGC Editors

We recently spoke to Gary Duncan and Wendy Russell of the Rowett Institute of Nutrition & Health in Aberdeen, Scotland, about the significance of phytochemical bioavailability to human health and the important role of liquid chromatography linked to tandem mass spectrometry (LC–MS-MS) in their research.

GC–MS Analysis of an Herbal Medicinal Remedy to Identify Potential Toxic Compounds

By Irene W. Kimaru, Hang P. Nguyen

In most countries, herbal medicinal products (HMPs) are introduced into the market without proper scientific evaluation or enforced safety and toxicological studies.

Sample Preparation Techniques

Simplifying Liquid Extractions

By Erica Pike

The benefits of supported liquid extraction (SLE) in sample cleanup and, in particular, the use of a synthetic SLE sorbent are discussed.

Pyrolysis for the Preparation of Macromolecules and Analysis by Gas Chromatography

By Douglas E. Raynie

When pyrolyzed, macromolecules will decompose into smaller fragments that can have the appropriate volatility for gas chromatography (GC) separation and analysis.

Understanding and Improving Solid-Phase Extraction

By Dawn Wallace Watson, Douglas E. Raynie

In this installment of "Sample Prep Perspectives," we cover some of the basic scientific principles behind solid-phase extraction (SPE) to allow the correct mode of extraction to be selected through an understanding of how analytes interact with and are separated by the sorbent.

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The LCGC Blog: Useful Tips for GC-MS

By Tony Taylor

There are many aspects of analytical science which abound with myth and legend – but gas chromatography – mass spectrometry (GC-MS), and more specifically the electron ionization (EI) process, stands out as the technique which has given rise to the largest number of ‘urban myths’ and misunderstandings.

The LCGC Blog: How to Get the Most Out of Your First Conference Experience

By Kevin A. Schug

In this blog installment, I would like to share a little advice with those of you who are attending your first major conference.

Latest News

Nominations Sought for 2017 Dal Nogare Award

The Chromatography Forum of the Delaware Valley is currently seeking nominations for the 2017 Dal Nogare Award.

Lung Cancer Breath Test in Clinical Trial

The University of Leicester and Glenfield Hospital in Leicester, UK, are starting a clinical trial into a gas chromatography–field asymmetric ion mobility spectrometry sensor (Owlstone Nanotech Ltd.) for the detection of early-stage lung cancer.

Thermo Fisher Scientific Buys Advanced Scientifics, Inc.

Thermo Fisher Scientific (Waltham, Massachusetts, USA) has acquired Advanced Scientifics, Inc. (ASI) (Millersburg, Pennsylvania, USA) for $300 million (US) in cash.

Determining Child Phthalate Exposure

Vinyl flooring in the home could be a key source of phthalates ingested and inhaled by children, according to a new study. The work was performed as part of an ongoing project by the Columbia Center for Children’s Environmental Health (CCCEH) at Columbia University in New York, USA, and followed a cohort of women and children living in New York city to investigate the impact of phthalate exposure on the health of children.

Joseph Jack Kirkland: LCGC’s 2015 Lifetime Achievement Award Winner

Joseph Jack Kirkland, considered by many to be one of fathers of high performance liquid chromatography (HPLC), is the winner of LCGC’s 2015 Lifetime Achievement Award.



Use of Novel Zirconia-Based Cleanup Sorbents for the Analysis of Contaminants in Fatty Food Samples

Tuesday, March 31, 2015 — 8:00 am PDT/ 11:00 am EDT/ 4:00 pm GMT/ 5:00 pm CEST


Simplifying Carbohydrate Testing in Food and Beverages to Meet Food Quality and Labeling Requirements Using Ion Chromatography and Pulsed Amperometric Detection

Thursday, April 2, 2015 — 8:00 am PDT/ 11:00 am EDT/ 4:00 pm BST/ 5:00 pm CEST


Trouble-Free HPLC Analysis of Creams and Lotions

Tuesday, April 7, 2015 — 8:00 am PDT / 11:00 am EDT / 4:00 pm BST / 5:00 pm CEST


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LCGC eBooks


Featured: Applications of Ion Chromatography

You know the classic uses of ion chromatography (IC), like measuring inorganic ions in drinking water. But do you know what else it can do?



HPLC Troubleshooting Guide to Cycling Baselines and Pressure Fluctuations

HPLC troubleshooting guide to cycling baselines and pressure fluctuations from LCGC's ChromAcademy.

My LC–MS isn’t behaving! Where do I start?

Instrument manufacturers try to convince us that mass spec is just another detector. Most of us who work with LC-MS know that’s simply not the case – they can be maintenance intensive, unforgiving and generate complex information. When they’re not working it can be difficult to work out exactly where the problem lies. Here’s some advice to point you in the right direction

HPLC Column Dead Volume

A question about column dead volume. If the hold-up volume (time) is the extra-column dead volume expressed in time or volume. What is the column dead volume and how can it be calculated?

Seven Must Know Points to Understand Atmospheric Pressure Chemical Ionization

CHROMacademy delivers key knowledge quickly in bite-size modules, and to prove this here is our seven point guide to understanding atmospheric pressure chemical ionization.

HPLC Extra Column Volumes

It is of particular importance to reduce extra column volumes when using small volume columns or UHPLC. However, where do these extra column volumes come from, how can they be minimized, and what effect do they have on chromatography?