Featured LCGC Interviews

Miniaturizing Biomarker Detection

The development of novel microfluidic systems opens up new opportunities to quantify clinically relevant biomolecules to further the understanding and diagnosis of disease. The 2015 recipient of the AES Mid-Career Award, Adam T. Woolley, from Brigham Young University, Utah, is working in this area to develop novel and sophisticated integrated microfluidic systems for enhanced biomarker quantitation and quantification. He recently spoke to LCGC about this work.

Liquid Chromatography

Questions of Quality: Where Can I Draw The Line?

By R.D. McDowall

A question that keeps raising its head when working in a regulated laboratory is can chromatographers integrate peaks manually? If they can, when can they do it? Also if they can manually integrate, when should they not do it?

Tips & Tricks GPC/SEC: Answering Common Questions About GPC/SEC Columns

By Daniela Held, Wolfgang Radke

A selection of commonly asked questions about GPC/SEC from users.

Internal Standard Calibration Problems

By John W. Dolan

Readers' questions regarding problems related to internal standard calibration of liquid chromatography methods are addressed.

Gas Chromatography

Separating and Identifying Trace-Level Chemicals in Wine by Headspace SPME with GC×GC–TOF MS

By Steve Smith, Laura McGregor, David Barden

Solid-phase microextraction (SPME) coupled with comprehensive two?dimensional gas chromatography and time-of-flight mass spectrometry (GC×GC–TOF-MS) can be used to detect trace-level fungicides and compounds responsible for undesirable attributes known as “organoleptic faults” in wine. This article explains more.

An Accurate-Mass Database for Screening Pesticide Residues in Fruits and Vegetables by Gas Chromatography–Time-of-Flight Mass Spectrometry

By Noelia Belmonte, Samanta Uclés, Miguel Gamón, Carmen Ferrer, Milagros Mezcua, Amadeo R. Fernández–Alba

The main objective of this study was to evaluate the capabilities of gas chromatography (GC) with time-of-flight mass spectrometry (MS) for screening pesticides in fruits and vegetables using a purpose-built accurate-mass database.

An Improved GC–MS Method for Cigarette Smoke Characterization Using a Novel Cold Trap, Dual Column, and Cryofocusing System

By M. Rotach, E. Rouget, J.R. Crudo

The development of a novel trapping system and a modified GC–MS layout (using dual chromatographic columns and cryogenic focusing devices) has enabled a major improvement in the chromatographic separation of volatile and semivolatile compounds in cigarette smoke. This improvement has led to the potential for identifying compounds which are usually masked by the solvent peak.

Mass Spectrometry

Single-Cell MS and High-Spatial-Resolution MS Imaging Under Ambient Conditions Using a Novel Sampling Device

By Ning Pan, Wei Rao, Zhibo Yang

We have developed a miniaturized, multifunctional device, called the "Single-Probe," that is capable of probing small targets and of sampling and ionizing molecular species.

Nonconventional Alternatives to LC–MS

By Sarah Trimpin, Shubhashis Chakrabarty, Beixi Wang, Khoa Hoang, Corinne A. Lutomski, Ellen D. Inutan, Charles N. McEwen

An important attribute of a novel ionization process for use in mass spectrometry (MS) is its simplicity and flexibility to be hyphenated to conventional liquid-based separation methods.

Quantifying Small Molecules by Mass Spectrometry

By P. Jane Gale, Alfred L. Yergey, Mark W. Duncan, Kate Yu

The use of a mass spectrometer in quantitative analysis exploits its exquisite selectivity and sensitivity as a detector, allowing a signal to be ascribed to a particular chemical entity with high certainty, even when present in a sample at a low concentration. There are, however, some special considerations that are necessary when a mass spectrometer is used as a quantitative tool.

Sample Preparation Techniques

Advances in Microsampling for In Vivo Pharmacokinetic Studies

By Nurith Amitai, Hong Xin, Daniel Kassel, Stuart Kushon

Volumetric absorptive microsampling (VAMS) is gaining traction because it delivers the benefits of dried blood spots (DBS) and overcomes its limitations while generating comparable PK data to conventional sampling methods. This article explains more.

Simplifying Liquid Extractions

By Erica Pike

The benefits of supported liquid extraction (SLE) in sample cleanup and, in particular, the use of a synthetic SLE sorbent are discussed.

Pyrolysis for the Preparation of Macromolecules and Analysis by Gas Chromatography

By Douglas E. Raynie

When pyrolyzed, macromolecules will decompose into smaller fragments that can have the appropriate volatility for gas chromatography (GC) separation and analysis.

Partner Organizations


Sepu, China's Largest Chromatography Training Site

About Sepu

News from Sepu


Chinese American Chromatography Association (CACA)

About CACA



ChromAcademy Homepage


The LCGC Blog: Why Every Good Analytical Chemist Also Needs to Be a Statistician

By Tony Taylor

Once you have mastered the terminology and symbology the actual mathematics for the models and approaches used at the level which is useful to practicing analytical chemists is really very straightforward indeed.

The LCGC Blog: Forensics, Lawyers, and Method Validation—Surprising Knowledge Gaps

By Kevin A. Schug

For the analytical community, method validation in some form or another is a natural extension of best practice in the analytical laboratory. However, the notion of method validation, and many aspects of detailed forensics analysis, are not well understood by most lawyers and judges.

Latest News

Methadone Linked to NDMA Contamination in Drinking Water

Methadone, a drug prescribed for managing the symptoms of heroin withdrawal and chronic pain, has been linked to the formation of the carcinogenic compound N-Nitrosodimethylamine (NDMA) in drinking water. Understanding which compounds contribute to NDMA formation in water could lead to tailored wastewater treatment approaches to remove the risk of contamination.

Quantification of Celiac Disease Gluten Epitopes

Scientists have published a novel method to detect multiple celiac disease-epitopes in wheat extracts simultaneously using liquid chromatography–multiple reaction monitoring mass spectrometry (LC–MRM–MS).

Palaeolithic Poisons Analysis

A new study hopes to highlight that poison was used as far back as 30,000 years ago. Dr Valentina Borgia, a specialist in Palaeolithic hunting weapons and Marie Curie Fellow at the McDonald Institute for Archaeological Research at the University of Cambridge, is using liquid chromatography combined with mass spectrometry (LC–MS) to analyze prehistoric weaponry.

Taylor Zhang Joins LCGC’s Editorial Advisory Board

LCGC Magazine is pleased to announce the addition of Taylor Zhang to its editorial advisory board.

Parabens Detected in Plastic Teething Toys

Parabens are commonly used in cosmetics and personal care products to protect against microbial growth, but a new study suggests that manufacturers may also be using parabens in plastic teething products designed for infants.


LCGC eBooks

Advancing Biopharma Analysis with Light-Scattering Detection

To characterize biopharmaceuticals, particularly monoclonal antibodies and antibody–drug conjugates (ADCs), you need a complete toolbox of powerful tools. You are probably familiar with LC–MS methods. But have you seen what light-scattering detection can do?

Go to E-Book Library>>



Helium to Hydrogen — A Change Would Do You Good

Change is always a scary concept but let us dispel some of the fear associated with changing your helium carrier gas to hydrogen by answering some of the most common questions posed to us.

Reversed-Phase HPLC for the Analysis of Biomolecules

The following article from LCGC's ChromAcademy introduces the fundamentals of biopharmaceutical analysis and cover the use of reversed-phase HPLC in the analysis of biomolecules.

HPLC Troubleshooting Guide to Cycling Baselines and Pressure Fluctuations

HPLC troubleshooting guide to cycling baselines and pressure fluctuations from LCGC's ChromAcademy.

My LC–MS isn’t behaving! Where do I start?

Instrument manufacturers try to convince us that mass spec is just another detector. Most of us who work with LC-MS know that’s simply not the case – they can be maintenance intensive, unforgiving and generate complex information. When they’re not working it can be difficult to work out exactly where the problem lies. Here’s some advice to point you in the right direction

HPLC Column Dead Volume

A question about column dead volume. If the hold-up volume (time) is the extra-column dead volume expressed in time or volume. What is the column dead volume and how can it be calculated?