As you hurry through your daily work in the analytical laboratory, are you failing to ask yourself some fundamental questions about the methods you use and the results you produce?
This collection of short articles from LCGC’s digital magazine, The Column, raises four key questions you should ask. And if the answers aren’t what you hoped, we offer advice for addressing the gaps.
Babies and infants experience rapid growth within a short timeframe and the nutrition that they absorb is therefore of the utmost importance. María Mateos-Vivas from the Department of Analytical Chemistry, Nutrition, and Food Science at the University of Salamanca, Spain, has used hydrophilic interaction liquid chromatography (HILIC) coupled to tandem mass spectrometry (MS/MS) to investigate the role of nucleotides on infant health. She recently spoke to us about this research.
The future of biological and clinical research will depend on technological innovations and cross discipline co-operation as science seeks a deeper understanding of increasingly complex biological systems. The 2016 recipient of the AES Mid-Career Award, Amy Herr, and her team at the University of California Berkeley have explored these areas using a combination of chemical engineering, mechanical engineering, and electrical engineering with strong foundations in biology, material science, and analytical chemistry to innovate new microfluidic analytical technology. She recently spoke to LCGC about this work.
Chromatography connected with ion mobility spectrometry (IMS) is not commonly used, but is being investigated more. IMS is an independent analytical technique with very good detectability and a rather small separation ability. One favourable property of IMS is that it can work with ambient pressure and can be easily connected to a gas chromatograph. Analytical applications of GC–MS are very different and encompass investigations into food, medical science, environment, drugs of abuse, chemical warfare agents, and explosives.
In clinical and forensic/toxicology laboratories, urine is a preferred matrix from which to quantify drug concentrations because it yields accurate results and allows for noninvasive collection methods. Prior to excretion, drug metabolites in the body undergo a glucuronidation reaction, resulting in a glucuronide bond that must be cleaved before mass spectrometry (MS) analysis by a β-glucuronidase enzyme hydrolysis. Many laboratories employ a “dilute-and-shoot” method after hydrolysis to decrease residual protein or enzyme concentration, but this method negatively affects column lifetime and reduces the sensitivity of analyte detection. By using a β-glucuronidase removal approach, analysts are able to see an increase in sensitivity and a reduction in MS instrument maintenance.
An LC–MS method for simultaneous quantification of buprenorphine and three metabolites: norbuprenorphine, buprenorphine glucuronide, and norbuprenorphine glucuronide.
Many of this year’s new products fit into recently identified trends, but one major driver of advances was not in our forecasts.
By Tony Taylor
The title of this piece may have put you off reading it – in which case you won’t be insulted when I say that even the most experienced gas chromatographers often fail to install columns in the best way possible.
Matrix-assisted laser desorption–ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is actually one of the most versatile tools for fingerprinting mixtures.
Seven outdated traditional practices that should not be performed without considering alternative approaches that can improve results, provide lower operation costs, or give faster run times. Instead of working harder, analytical scientists should work smarter. Learn more by clicking through the slideshow.
Webster’s New Collegiate Dictionary defines a myth as “an ill-founded belief held uncritically, especially by an interested group.” Could that group be misinformed chromatographers?
The University of Manchester unveiled the £18-million Stoller Biomarker Discovery Centre at the beginning of June 2016. The research centre will focus on biomedical research on major diseases, including cancer, psoriasis, and arthritis, using mass spectrometry (MS)‑based proteomics solutions from Sciex.
Researchers from Vienna University of Technology (TU Wien), Austria, have used chip capillary electrophoresis (CE) in combination with molecular beacons (MBs) to analyze the release of the RNA genome from a human rhinovirus.
Thursday, July 26th, 2016
8 am PDT / 11 am EDT / 4 pm BST / 5 pm CEST
Thursday, August 4th, 2016
8 am PDT / 11 am EDT / 4 pm BST / 5 pm CEST
The presence of pesticides and other chemical contaminants in food and dietary supplements is an important concern for both health and environmental reasons. In this new e-book, developed in collaboration with the organizers of the North American Chemical Residues Workshop (NACRW), four leading experts in the field, all frequent speakers at the conference, share some of their latest work and findings.
CHROMacademy's Tutor Scott Fletcher discusses whether HPLC method development has become a dying art.
Selecting a gas chromatography (GC) column can be a daunting task. It may seem like there are a never-ending number of phase chemistries, or an inordinate number of column geometry options. However, when choosing a column for a new application (or to improve an existing one) there are some simple rules that can be followed.