Featured LCGC Interviews

Environmental Forensics in the Workplace

Populations worldwide are exposed to polychlorinated biphenyls (PCBs) that can be harmful to human health. The Column spoke to David Megson from Ryerson University in Toronto, Canada, about recent developments in assessing human exposure to PCBs and chiral enantiomer fractions (EFs) in the workplace.

Liquid Chromatography

Characterizing Therapeutic Monoclonal Antibodies

By Chris Pohl

Accounting for the inherent heterogeneity of monoclonal antibodies is essential to ensure production of consistent and safe therapeutics and, in light of this, there is an array of chromatographic and supporting regulatory systems. Robust characterization and analysis of monoclonal antibodies is becoming a fast and straightforward process thanks to the myriad technological advances underpinning this rapidly evolving area.

Analyzing Host Cell Proteins Using Off-Line Two-Dimensional Liquid Chromatography–Mass Spectrometry

By Koen Sandra, Alexia Ortiz, Pat Sandra

The use of off-line 2D-LC–MS for the characterization of HCPs and their monitoring during downstream processing.

Advances in Liquid Chromatography–Tandem Mass Spectrometry (LC–MS–MS)-Based Quantitation of Biopharmaceuticals in Biological Samples

By Nico C. van de Merbel

The technical requirements for a successful LC–MS–MS method for the quantitation of biopharmaceuticals are presented and the advantages and disadvantages compared to ligand-binding assays are evaluated.

Gas Chromatography

The Essentials: Optimizing Sample Introduction for Headspace GC

By LCGC Editors

The Essentials: Optimizing Sample Introduction for Headspace GC

Split-Vent Traps: GC's Dirty Secret

By John V. Hinshaw

A look at the the operation and maintenance of split-vent traps.

Developments in Gas Chromatography Using Ionic Liquid Stationary Phases

By Chadin Kulsing, Yada Nolvachai, Helmut M. Hügel, Philip J. Marriott

Ionic liquids (ILs) have become recognized in gas chromatography (GC) as stable and highly polar stationary phases with a wide application range. Having customizable molecular structures, ILs also offer a particular tunability that provides additional selectivity, and therefore may improve separation for neighbouring analytes. This article presents specific properties of IL phase capillary GC columns, including polarity scale and inner surface morphologies of IL columns. Application of IL phases in achiral and chiral GC, and multidimensional GC, are highlighted.

Mass Spectrometry

MS Detection of Waste in Public Swimming Facilities

By Heather L. Rudolph, Troy D. Wood

Is your swimming pool clean and safe? Recreational water illness, most commonly in the form of digestive tract illness or skin, ear, or respiratory infections, is often caused by water contamination. The authors present a robust method, using solid-phase extraction and high-resolution mass spectrometry, for monitoring swimming pool water.

Harnessing the Benefits of Mass Spectrometry for In-depth Antibody Drug Conjugates Analytical Characterization

By Alain Beck, Sarah Cianferani

Recent progress in high-resolution mass spectrometry (HRMS) and liquid chromatography–mass spectrometry (LC–MS) methods for the structural characterization of brentuximab vedotin and trastuzumab emtansine are presented.

Higher Order Mass Spectrometry Techniques Applied to Biopharmaceuticals

By Christian Huber

An outline of the basic principles of MS techniques used to investigate higher order structural features of biopharmaceuticals, as well as some insights into applications relevant to the pharmaceutical industry.

Sample Preparation Techniques

Let’s Do It Right the First Time: The Importance of Solvent Safety Considerations

By Doug Raynie

It's easy to get lax about safety, but the risks are too high.

Solid-Phase Microextraction in Clinical Diagnostics

By Bethany Degg

Researchers at the University of Waterloo in Canada are collaborating with clinicians at Toronto General Hospital to develop preclinical and clinical applications of solid-phase microextraction (SPME). Bethany Degg of The Column spoke to Barbara Bojko from the team to find out more.

Advances in Sample Preparation: Removing Phospholipids from Biological Samples

By Jonathan P. Danaceau, Xin Zhang, Pamela Iraneta, Michael Young, Kimvan Tran, Kim Haynes, Erin Chambers

Solid-phase extraction (SPE) has long been used for sample preparation — and is often chosen over liquid–liquid extraction (LLE) — because it can efficiently clean up and concentrate analytes of interest without the use of the more toxic organic solvents. This article describes the properties and use of a new SPE sorbent that removes phospholipids from biological samples, and illustrates the benefits of this approach with practical examples.

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The LCGC Blog: GC Troubleshooting in 20 Pictures (Part 1)

By Tony Taylor

They say a picture paints a thousand words… This month I’ve taken inspiration from a recent webcast, presented at www.chromacademy.com, in which I presented real data from our work that represents some ‘classic’ GC problems.

The LCGC Blog: Column Overload in Gas Chromatography with Vacuum Ultraviolet Detection

By Kevin A. Schug

Column overload is a very commonly encountered issue in GC for beginners. Changes in peak symmetry, generally observed as peak fronting, can be subtle in the sharp peaks generated by GC, but the result can be significant shifts in retention times, loss of resolution, and error in peak integration.


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LCGC Slideshows

Slideshow: Seven Common Faux Pas in Modern HPLC

Seven outdated traditional practices that should not be performed without considering alternative approaches that can improve results, provide lower operation costs, or give faster run times. Instead of working harder, analytical scientists should work smarter. Learn more by clicking through the slideshow.

Slideshow: The Top 10 HPLC and UHPLC Column Myths

Webster’s New Collegiate Dictionary defines a myth as “an ill-founded belief held uncritically, especially by an interested group.” Could that group be misinformed chromatographers?

Latest News

Analyzing Edvard Munch

The characterization of paint in famous artworks is valuable for investigating the painting techniques used by artists, for conservation purposes and to help repair damaged paintings. By combining HPLC–ESI-QTOF-MS with traditional analytical techniques, researchers were able to clarify the composition of the oil components and hypothesize the botanical origin of the lipid materials used by Edvard Munch.

Biomonitoring POPs Using Fish Bile

A new method for the simultaneous determination of emerging and persistent organic pollutants (POPs) in fish bile samples has been developed using gas chromatography–mass spectrometry (GC–MS).

Phenomenex Expands Method Development Services

Phenomenex has announced that PhenoLogix, the company’s in-house analytical services group, has moved to an expanded, stand-alone facility.

In Memory of Roy Eksteen

Roy Eksteen, a passionate chromatographer and beloved member of the separation science community, passed away on September 21, 2015.

Mars to Open Food Safety Center in China

Mars, Incorporated (McLean, Virginia, USA) has announced the opening of its Global Food Safety Center, a first-of-its-kind facility for pre-competitive research and training that aims to raise global food safety standards through collaboration.


Streamlining Routine Workflows in the Environmental Laboratory

Part 1, Live: Wednesday, September 30, 2015 11am EDT/ 8am PDT/ 4pm BST/ 5pm CEST

Part 2, Live: Wednesday, Oct. 28, 2015 11am EDT/ 8am PDT/ 3pm GMT/ 4pm CET


Overview of Hydrophilic Interaction Chromatography (HILIC)

Tuesday, October 20, 2015 — 2:00-3:00pm EDT 

Part 2, Live: Wednesday, Oct. 28, 2015 11am EDT/ 8am PDT/ 3pm GMT/ 4pm CET


Mass Detection for The Masses: How Compact Mass Detectors Are Improving the Analysis of Herbal and Dietary Supplements

Wednesday, October 21, 2015 1pm EDT/ 12pm CDT/ 10am PDT 


More Upcoming and On Demand Webcasts>>

LCGC eBooks

Pesticide Residue Analysis: Tips and Tricks for the Whole Workflow

This e-book, based on a series of four web seminars, provides pesticide residue analysts with valuable information from Thermo Fisher Scientific about the development and optimization of methods and workflows for the analysis of pesticide residues in food.

Go to E-Book Library>>



GC Troubleshooting in 20 Pictures (Part 1)

They say a picture paints a thousand words…This month I’ve taken inspiration from a recent webcast, presented at www.chromacademy.com, in which I presented real data from our work that represents some ‘classic’ GC problems.

Static Headspace Sampling

Static headspace sampling is typically used for the determination of volatile and semi-volatile analytes in liquids and, more rarely, solid matrices. Application examples include the analysis of alcohols in blood, residual solvents in pharmaceuticals, flavours and taints in food and beverages as well as fragrances in perfumes and detergents.

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