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Features
Gradient Elution, Part II: Equivalent Separations
Is it possible to get the same separation under isocratic and gradient conditions?
Influence of Sample Solvent Composition for SFC Separations
A discussion of different sample solvent compositions to be injected for SFC as part of injection linearity experiments
The Early Development of Size Exclusion Chromatography: A Historical Perspective
In this month's column, Howard Barth traces the early development of SEC
Automated MAb Workflow: From Harvest Cell Culture to Intact Mass Analysis of Variants
Recombinant monoclonal antibodies (MAbs) are a growing class of therapeutic molecules. Early in the development of MAbs, a large number of harvest cell culture (HCC) samples must be screened for immunoglobulin G (IgG) titer, aggregations, and charge variants. This study describes a two-dimensional (2D) high performance liquid chromatography (HPLC) workflow for afinity, size-exclusion, and ion-exchange analysis.
Practical and Popular Sample Preparation Techniques for Ion Chromatography
The authors present the most common and fundamental techniques that address common matrix issues and discuss the critical chemistry considerations.

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