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Automated MAb Workflow: From Harvest CellCulture to Intact Mass Analysis of Variants
Recombinant monoclonal antibodies (MAbs) are a growing class of therapeutic molecules. Early in the development of MAbs, a large number of harvest cell culture (HCC) samples must be screened for immunoglobulin G (IgG) titer, aggregations, and charge variants. This study describes a two-dimensional (2D) high performance liquid chromatography (HPLC) work ow for af nity, size-exclusion, and ion-exchange analysis.
Practical and Popular Sample Preparation Techniques for Ion Chromatography
The authors present the most common and fundamental techniques that address common matrix issues and discuss the critical chemistry considerations.
Carbon Nanotubes: Applications in Chromatography and Sample Preparation
This article gives an overview of research into carbon nanotubules and their potential applications in chromatography and sample preparation.
New Chromatography Columns and Accessories at Pittcon 2013, Part I
Part I of our yearly report, covering columns for all modes of HPLC, UHPLC, and liquid-phase separations.
Assay for Parts-Per-Million Levels of Azide in Drug Substances
The removal of azide as a potential impurity from a drug substance may be critical to its safety profile. The quantitation of thi impurity is an important control parameter. This article describes a simple and practical assay for azide using chemical derivatization and HPLC.

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