Liquid Chromatography

Large-Volume Injection LC–MS-MS Methods for Aqueous Samples and Organic Extracts

By Jennifer A. Field, Matthew J. Perkins, B. McKay Allred

Environmental sample analysis by large-volume injection (LVI) in combination with liquid chromatography–tandem mass spectrometry (LC–MS-MS) is described for polar and nonpolar analytes in both aqueous samples and organic extracts.

Seven Things to Avoid in the Liquid Chromatography Laboratory

By John W. Dolan

Have a problem with your LC system? Maybe applying a little "DDT (Don't Do That)" may help.

The 2014 Emerging Leader in Chromatography: Andre de Villiers

By LCGC Editors

LCGC recently spoke with the 2014 LCGC Emerging Leader in Chromatography award winner, Andre de Villiers, an Associate Professor of Science at Stellenbosch University in South Africa, about his current research analyzing natural products, his various interests and collaborations in analytical chemistry, and his role as a teacher.

Gas Chromatography

GC Detectors: From Thermal Conductivity to Vacuum Ultraviolet Absorption

By Kevin A. Schug, Harold M. McNair

Some might consider gas chromatography (GC) a mature technique. However, several substantial advances in GC technology in the past few years have proven that there is still room for innovation.

Critical Evaluation of GC Methods

By Tony Taylor

How to spot weaknesses in methods before problems occur

Electronic Control of Carrier Gas Pressure, Flow, and Velocity

By John V. Hinshaw

How does a GC system set and control gas pressures, flows, and carrier gas velocities electronically?

Mass Spectrometry

Application of Ambient Sampling Portable Mass Spectrometry Toward On-Site Screening of Clandestine Drug Operations

By Seth E. Hall, Christopher C. Mulligan

Worldwide trends in illicit drug use and production have shifted toward an increase in synthetic analogues and the emergence of new variations in their manufacture.

Unraveling the Links Between Diet and Human Health Using LC–MS-MS

By LCGC Editors

We recently spoke to Gary Duncan and Wendy Russell of the Rowett Institute of Nutrition & Health in Aberdeen, Scotland, about the significance of phytochemical bioavailability to human health and the important role of liquid chromatography linked to tandem mass spectrometry (LC–MS-MS) in their research.

Simultaneous Determination of Methylxanthines and Cotinine in Human Plasma by Solid-Phase Extraction Followed by LC–MS-MS

By Rossana Bossi, Bodil Hammer Bech

A multiresidue method has been developed and validated for the analysis of methylxanthines (caffeine and its metabolites) and cotinine in human plasma.

Sample Preparation Techniques

Pyrolysis for the Preparation of Macromolecules and Analysis by Gas Chromatography

By Douglas E. Raynie

When pyrolyzed, macromolecules will decompose into smaller fragments that can have the appropriate volatility for gas chromatography (GC) separation and analysis.

Understanding and Improving Solid-Phase Extraction

By Douglas E. Raynie, Dawn Wallace Watson

In this installment of "Sample Prep Perspectives," we cover some of the basic scientific principles behind solid-phase extraction (SPE) to allow the correct mode of extraction to be selected through an understanding of how analytes interact with and are separated by the sorbent.

Developments in Green Chromatography

By Jacek Namiesnik, Marek Tobiszewski

Green analytical chemistry is a widely recognized concept that has led to the development of new analytical methods with reduced environmental impact and minimized analyst occupational exposure. This article presents the most recent progress in the development of greener sample preparation and chromatographic separation techniques.

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GC Column Conditioning Stop Wasting Time and Money!

By Tony Taylor

If you do Gas Chromatography, then you will have undoubtedly conditioned a capillary GC column ready for use after a period of storage or before first use.

Insights on Increased Efficiency for Superficially Porous Particles Among Other Things

By Kevin A. Schug

I have had enough conversations with experts in the field of high performance liquid chromatography (HPLC) stationary-phase supports to know that there is more to the increased efficiency provided by the use of superficially porous particles (SPP) compared to fully porous particles (FPP) than simply mass transfer effects. Yet, I would argue that this is still one of the biggest misconceptions propagated by some members of the chromatography community.

Latest News

Analyzing Honey Bee Immunity With Nano LC–MS

A new study published in the Journal of Chromatography A presents a sensitive method for the quantification of apidaecins, a type of antimicrobial peptide that play an important role the immune response of honey bees to pathogens. The method was developed using reversed-phase nanoliquid chromatography coupled with high resolution mass spectrometry (LC–MS) to determine apidaecins in hemolymph or body parts from individual honey bees.

Characterizing Food Packaging Materials

A group of scientists from the University of Parma in Italy, has performed capillary ultrahigh-performance liquid chromatography and electrospray ionization quadrupole orbitrap high-resolution mass spectrometry (UHPLC–ESI Q-orbitrap–HRMS) to characterize materials used in food packaging and that consequently come into contact with food, namely polycarbonate food-contact plastics.1

Detecting Nerve Agent Exposure

Scientists at the Norwegian Defence Research Establishment (Kjeller, Norway) are working in collaboration with the University of Oslo (Oslo, Norway) to develop new methods to detect the illicit use of nerve agents. In a recent study published in the journal Analytical Chemistry, the team presented a novel method for the quantification of nerve agent metabolites in human serum and urine by combining salting-out assisted liquid–liquid extraction (SALLE) and on-line solid-phase extraction–liquid chromatography–tandem mass spectrometry (SPE–LC–MS–MS).

Speeding Up Drug Metabolism Screening

A new study published in the journal Analytical Chemistry presents a drug metabolism strategy based on microsome mesoporous organosilica nanoreactors coupled with high performance liquid chromatography–mass spectrometry (HPLC–MS) to screen for potential drug toxicity.

Metabolic Profiling of Tea

A new study published in the Journal of Chromatography A presents metabolite profiling of tea (Camella sinensis) harvested from the Bulang Mountains in Yunnan, China, using multidimensional gas chromatography–mass spectrometry (GC×GC–MS) to determine the impact of seasonal changes.


Make Your Lab Productivity Soar With the Right Chromatography Data System

Thursday, January 27, 2015 —8:00 am PST/ 10:00 am CST/ 11:00 am EST


Characterization of Functionalized Nanoparticles Using Ambient Ionization Mass Spectrometry

Wednesday, January 28, 2015 —8:00 am PST/ 11:00 am EST/ 4 pm GMT/ 5 pm CET


Editors' Series: Applications of Native MS and High Resolution Mass Spectrometry for the Characterization of Biotherapeutic Molecules

Thursday, January 29, 2015 —8:00 am PST/ 10:00 am CST/ 11:00 am EST


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LCGC eBooks

Featured: Analytical Tools for Biopharmaceutical Characterization: 

In this new e-book, leading experts present the latest analytical tools for characterizing biopharmaceuticals, including chromatographic methods, mass spectrometry methods, and focused methods for analyzing carbohydrates on therapeutic glycoproteins.



Seven Must Know Points to Understand Atmospheric Pressure Chemical Ionization

CHROMacademy delivers key knowledge quickly in bite-size modules, and to prove this here is our seven point guide to understanding atmospheric pressure chemical ionization.

HPLC Extra Column Volumes

It is of particular importance to reduce extra column volumes when using small volume columns or UHPLC. However, where do these extra column volumes come from, how can they be minimized, and what effect do they have on chromatography?