Featured LCGC Interviews

Untargeted Metabolomics for Marine Pollution Analysis Using GC–MS

Marine polychaetes are a common type of annelid worm widely spread in marine environments. Raquel Fernandez from the University of Copenhagen, Denmark, spoke to Aladair Matheson of The Column about her innovative approach to developing an untargeted method to monitor polychaetes and assess their potential use in environmental monitoring of oil spills.

Liquid Chromatography

Removal of Contaminant Peaks in Reversed-Phase Gradient Liquid Chromatography for Improved Detection of Pharmaceutical Impurities

By Mark R. Taylor, Fiona Harvey-Doyle, Krina Patel

The authors present a method for comparing the levels of baseline interference arising from common laboratory mobile phase contamination sources and assess different approaches for removing dissolved contaminants to generate interference free chromatogram baselines. This article demonstrates that recirculating mobile phase through a semi-preparative scale column using a reagent delivery pump to be more effective than previously published mobile phase decontamination methods.

Biopharmaceutical Candidate Screening with Automated Dynamic Light Scattering

By Daniel Some

Automated high-throughput dynamic light scattering (HT–DLS) can enable rapid and unattended assessment of sample purity in biopharmaceutical samples, maximizing the reliability and productivity of the candidate selection process. This article explains more.

Gradient HPLC—One Invaluable Equation

By Tony Taylor

Here, we concentrate on one particularly useful equation that allows us to make changes to an analytical system to improve throughput or efficiency, while retaining the selectivity of the original method.

Gas Chromatography

Developments in Gas Chromatography Using Ionic Liquid Stationary Phases

By Chadin Kulsing, Yada Nolvachai, Helmut M. Hügel, Philip J. Marriott

Ionic liquids (ILs) have become recognized in gas chromatography (GC) as stable and highly polar stationary phases with a wide application range. Having customizable molecular structures, ILs also offer a particular tunability that provides additional selectivity, and therefore may improve separation for neighbouring analytes. This article presents specific properties of IL phase capillary GC columns, including polarity scale and inner surface morphologies of IL columns. Application of IL phases in achiral and chiral GC, and multidimensional GC, are highlighted.

A Decade of Quantitating Cyanide in Aqueous and Blood Matrices Using Automated Cryotrapping Isotopic Dilution Static Headspace GC–MS

By Paul R. Loconto

Some insights into the principles and practice of implementing and improving on methods to quantitate cyanide at trace concentration levels in human blood.

Flame Ionization: GC's Workhorse Detector

By John V. Hinshaw

FID continues to play a crucial role for all types of gas chromatography, so it is important to understand how to use it effectively.

Mass Spectrometry

GC–MS Analysis of Aroma Compounds in Edible Oils by Direct Thermal Desorption

By Alexander Hasselbarth, Oliver Lerch

Determining aroma compounds and off-flavours in edible oils by gas chromatography coupled to mass spectrometry (GC–MS) is routine in many food-producing companies; however, the oily matrix needs to be kept out of the analytical instrument to avoid impacting analytical stability. The following article describes a simple, yet efficient, way of eliminating the matrix while determining the flavour compounds in a sensitive and selective manner.

Single-Cell MS and High-Spatial-Resolution MS Imaging Under Ambient Conditions Using a Novel Sampling Device

By Ning Pan, Wei Rao, Zhibo Yang

We have developed a miniaturized, multifunctional device, called the "Single-Probe," that is capable of probing small targets and of sampling and ionizing molecular species.

Nonconventional Alternatives to LC–MS

By Sarah Trimpin, Shubhashis Chakrabarty, Beixi Wang, Khoa Hoang, Corinne A. Lutomski, Ellen D. Inutan, Charles N. McEwen

An important attribute of a novel ionization process for use in mass spectrometry (MS) is its simplicity and flexibility to be hyphenated to conventional liquid-based separation methods.

Sample Preparation Techniques

Solid-Phase Microextraction in Clinical Diagnostics

By Bethany Degg

Researchers at the University of Waterloo in Canada are collaborating with clinicians at Toronto General Hospital to develop preclinical and clinical applications of solid-phase microextraction (SPME). Bethany Degg of The Column spoke to Barbara Bojko from the team to find out more.

Advances in Sample Preparation: Removing Phospholipids from Biological Samples

By Jonathan P. Danaceau, Xin Zhang, Pamela Iraneta, Michael Young, Kimvan Tran, Kim Haynes, Erin Chambers

Solid-phase extraction (SPE) has long been used for sample preparation — and is often chosen over liquid–liquid extraction (LLE) — because it can efficiently clean up and concentrate analytes of interest without the use of the more toxic organic solvents. This article describes the properties and use of a new SPE sorbent that removes phospholipids from biological samples, and illustrates the benefits of this approach with practical examples.

Aiding the Development of Extraction Procedures with Response Surface Methodology

By John Kiratu, Douglas E. Raynie

With response surface methodology, one can screen and optimize several extraction methods simultaneously. Here’s how.

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The Acid Test - More Useful Calculations for HPLC Eluent Preparation

By Tony Taylor

Mobile-phase preparation for LC–MS requires careful consideration to ensure the correct pH values and concentrations are obtained. This blog installment details the difference between using volume percent and weight percent to to make up 0.1% solutions of trifluoroacetic acid and the effect it has on the pH.

The LCGC Blog: An Open Inquiry Model for Science Education

By Kevin A. Schug

There is a shortcoming in our current educational system. There is too much rote learning, and not enough time given to let science-minded students explore a topic. Overall, when students ask their own questions (not ones given to them by instructors), they become more invested in finding the answers.


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LCGC Slideshows

Slideshow: Seven Common Faux Pas in Modern HPLC

Seven outdated traditional practices that should not be performed without considering alternative approaches that can improve results, provide lower operation costs, or give faster run times. Instead of working harder, analytical scientists should work smarter. Learn more by clicking through the slideshow.

Slideshow: The Top 10 HPLC and UHPLC Column Myths

Webster’s New Collegiate Dictionary defines a myth as “an ill-founded belief held uncritically, especially by an interested group.” Could that group be misinformed chromatographers?

Latest News

Wheaton and Duran Group, Two Laboratory Consumables Providers, Merge

Wheaton (Millville, New Jersey), a US-based provider of laboratory glassware and consumables, has been acquired by Duran Group (Wertheim, Germany), a European provider of laboratory glassware.

Purnendu “Sandy” Dasgupta Awarded $1 Million NASA Grant

Purnendu “Sandy” Dasgupta, Hamish Small Chair in Ion Analysis of Chemistry and Biochemistry in the UT Arlington College of Science, has been awarded almost $1 million from NASA. The grant will support research into extension of the open-tubular capillary chromatography platform that Dasgupta developed for the detection and separation of ions.

Phenomenex and Tecan Announce Marketing Partnership

Phenomenex (Torrance, California, USA) and the Tecan Group (Männedorf, Switzerland) have announced a new collaboration to co-market automated solid-phase extraction (SPE) products.

Agilent Collaborates with Weill Cornell Medical College on ALS Research

Agilent Technologies Inc. (Santa Clara, California) has agreed to support research by Steven Gross, a faculty member in the Department of Pharmacology at Weill Cornell Medical College (New York, New York), into amyotrophic lateral sclerosis (ALS), also known as Lou Gehrig’s disease.

LC–MS Analysis of Rock Cores

Microbial life is possible within the Earth’s mantle, according to new research. High performance liquid chromatography–mass spectrometry (HPLC–MS) analysis of rock cores sampled from the Iberian plate margin (off the coast of Spain and Portugal) revealed the presence of lipid biomarkers indicative of microbial communities present beneath the seafloor more than 100 million years ago.


Streamlining Routine Workflows in the Environmental Laboratory

Part 1, Live: Wednesday, September 30, 2015 11am EDT/ 8am PDT/ 4pm BST/ 5pm CEST

Part 2, Live: Wednesday, Oct. 28, 2015 11am EDT/ 8am PDT/ 3pm GMT/ 4pm CET


Overview of Hydrophilic Interaction Chromatography (HILIC)

Tuesday, October 20, 2015 — 2:00-3:00pm EDT 

Part 2, Live: Wednesday, Oct. 28, 2015 11am EDT/ 8am PDT/ 3pm GMT/ 4pm CET


Mass Detection for The Masses: How Compact Mass Detectors Are Improving the Analysis of Herbal and Dietary Supplements

Wednesday, October 21, 2015 1pm EDT/ 12pm CDT/ 10am PDT 


More Upcoming and On Demand Webcasts>>

LCGC eBooks

gradient ebook LCGC

The Secrets to Successful Gradient Separations

Liquid chromatography separations using gradient elution have the potential to be faster, give better detection limits, and have more flexibility than separations using isocratic elution. However, problems can occur with gradients. Some of these problems are inherent to the technique but can be avoided or accommodated with a little care. Others result from a poor understanding of gradient elution. In this new e-book, John Dolan, renowned LC expert and the longtime author of the "LC Troubleshooting" column in LCGC, presents the secrets to achieving success with gradient separations.

Go to E-Book Library>>



Static Headspace Sampling

Static headspace sampling is typically used for the determination of volatile and semi-volatile analytes in liquids and, more rarely, solid matrices. Application examples include the analysis of alcohols in blood, residual solvents in pharmaceuticals, flavours and taints in food and beverages as well as fragrances in perfumes and detergents.

Fundamentals of GC–MS Ionisation Techniques

Understanding how an instrument works is great, but when do you have time to read a textbook, or go to a course? Let CHROMacademy help. Dip in and out of concise modules and take 5 minutes to learn a little about the fundamentals of your analytical technique. Why not delve into GC-MS ionisation techniques right now?