Featured LCGC Interviews

Developments in Dioxin Analysis

Bryan Vining from SGS Environmental Service (Wilmington, North Carolina, USA) reveals some of the cutting-edge research his team has performed involving dioxin analysis, and proposes some future possibilities for the direction of this field.

Liquid Chromatography

Food Allergen Analysis Using LC–MS–MS

By Ashley Sage

Changes to the European Food Safety Authority (EFSA) regulations on food labelling, and the strengths and limitations of new and existing methods for the detection of allergens in food are discussed in this article.

Fast and Simple Determination of Free Amino Acids in Beer

By Gesa J. Schad and Brigitte Bollig

A rapid and reliable high performance liquid chromatography–mass spectrometry (HPLC–MS) method to determine amino acids in beer is presented.

New Chromatography Columns and Accessories for 2015

By M. Swartz

This is our annual review of new liquid chromatography (LC) columns and accessories introduced at Pittcon and throughout the previous year.

Gas Chromatography

Separating and Identifying Trace-Level Chemicals in Wine by Headspace SPME with GC×GC–TOF MS

By Steve Smith, Laura McGregor, David Barden

Solid-phase microextraction (SPME) coupled with comprehensive two?dimensional gas chromatography and time-of-flight mass spectrometry (GC×GC–TOF-MS) can be used to detect trace-level fungicides and compounds responsible for undesirable attributes known as “organoleptic faults” in wine. This article explains more.

An Accurate-Mass Database for Screening Pesticide Residues in Fruits and Vegetables by Gas Chromatography–Time-of-Flight Mass Spectrometry

By Noelia Belmonte, Samanta Uclés, Miguel Gamón, Carmen Ferrer, Milagros Mezcua, Amadeo R. Fernández–Alba

The main objective of this study was to evaluate the capabilities of gas chromatography (GC) with time-of-flight mass spectrometry (MS) for screening pesticides in fruits and vegetables using a purpose-built accurate-mass database.

An Improved GC–MS Method for Cigarette Smoke Characterization Using a Novel Cold Trap, Dual Column, and Cryofocusing System

By M. Rotach, E. Rouget, J.R. Crudo

The development of a novel trapping system and a modified GC–MS layout (using dual chromatographic columns and cryogenic focusing devices) has enabled a major improvement in the chromatographic separation of volatile and semivolatile compounds in cigarette smoke. This improvement has led to the potential for identifying compounds which are usually masked by the solvent peak.

Mass Spectrometry

Nonconventional Alternatives to LC–MS

By Sarah Trimpin, Shubhashis Chakrabarty, Beixi Wang, Khoa Hoang, Corinne A. Lutomski, Ellen D. Inutan, Charles N. McEwen

An important attribute of a novel ionization process for use in mass spectrometry (MS) is its simplicity and flexibility to be hyphenated to conventional liquid-based separation methods.

Quantifying Small Molecules by Mass Spectrometry

By P. Jane Gale, Alfred L. Yergey, Mark W. Duncan, Kate Yu

The use of a mass spectrometer in quantitative analysis exploits its exquisite selectivity and sensitivity as a detector, allowing a signal to be ascribed to a particular chemical entity with high certainty, even when present in a sample at a low concentration. There are, however, some special considerations that are necessary when a mass spectrometer is used as a quantitative tool.

Unraveling the Links Between Diet and Human Health Using LC–MS-MS

By Chromatography Editors

We recently spoke to Gary Duncan and Wendy Russell of the Rowett Institute of Nutrition & Health in Aberdeen, Scotland, about the significance of phytochemical bioavailability to human health and the important role of liquid chromatography linked to tandem mass spectrometry (LC–MS-MS) in their research.

Sample Preparation Techniques

Solid Phase Extraction and Analysis Of Organochlorine Pesticides and inline Cleanup from Water

By Philip Bassignani

In water samples, OCPs are monitored by the U.S. Environmental Protection Agency by various methods including methods EPA 508, EPA 608 and EPA 8081. The use of Solid Phase Extraction (SPE) can rapidly increase both extraction efficiency and reduce lab solvent usage. By implementing the FMS TurboTrace® ABN system with its dual cartridge functionality, an in-line Florisil cartridge can be added to perform automated extract clean-up during the elution step.

Solid Phase Extraction and Analysis Of Organochlorine Pesticides and inline Cleanup from Water

By Philip Bassignani

In water samples, OCPs are monitored by the U.S. Environmental Protection Agency by various methods including methods EPA 508, EPA 608 and EPA 8081. The use of Solid Phase Extraction (SPE) can rapidly increase both extraction efficiency and reduce lab solvent usage. By implementing the FMS TurboTrace® ABN system with its dual cartridge functionality, an in-line Florisil cartridge can be added to perform automated extract clean-up during the elution step.

Determination of 11-nor-9-Carboxy-THC in Human Urine by QuEChERS and LC–MS–MS

By Xiaoyan Wang

11-nor-9-Carboxy-THC (also known as THCA or carboxy-THC) is the main secondary metabolite of THC. THCA is not psychoactive but has a long half-life. Due to this attribute, determination of THCA in urine plays an important role in the confirmation of marijuana consumption. This application utilizes the QuEChERS sample preparation technique to effectively quantitate THCA levels in human urine.

Partner Organizations

 

Sepu, China's Largest Chromatography Training Site

About Sepu

News from Sepu

 

Chinese American Chromatography Association (CACA)

About CACA

 

ChromAcademy

ChromAcademy Homepage

LCGC Blog

The LCGC Blog: Troubleshooting Retention Time Issues in Reversed Phase HPLC

By Tony Taylor

While some of the relationships between parameters in gradient HPLC are complex, others are reasonably straightforward. Here, Tony Taylor explains, through an example, how adjusting the flow rate can improve resolution between critical peak pairs.

The LCGC Blog: A Disconnect Between Science Research and Science Education Research

By Kevin A. Schug

Now that I have been at UT Arlington for 10 years, I have had many opportunities to refine my own approaches to teaching, in hopes that the learning curve to achieve meaningful learning for students is not as steep as the one I had to manage.

Latest News

Digital Microfluidics Enables Analysis of Multiple Steroid Hormones in Core-Needle Biopsy Samples

Researchers from the University of Toronto in Canada have developed a novel approach using digital microfluidics to analyze multiple steroid hormones in small tissue samples (1–10 mg) collected using core-needle biopsy (CNB) sampling.

VWR Acquires Hichrom

VWR, a global distributor of analytical products headquartered in Pennsylvania, USA, has announced its acquisition of the analytical instrumentation manufacturer Hichrom Limited based in Berkshire, UK.

Gyula Vigh Receives Arnold O. Beckman for Separation Techniques

Gyula Vigh, Emeritus Professor of Chemistry and inaugural holder of the Gradipore Chair in Separation Science at Texas A&M, has been awarded the Arnold O. Beckman Award for Outstanding Scientific Achievements in the field of electrodriven separation techniques sponsored by Sciex Separations, a division of AB Sciex (Massachusetts, USA).

Miniaturized High-Performance Chiral Liquid Chromatography

Stereoisomers of chiral compounds — two different forms (isomers) that are identical in composition but are non‑superimposable mirror images of one another — can have dramatically different effects in the human body making it important to ensure the purity of enantiomeric mixtures produced in drug development.

Investigating the Link Between PBDE Exposure and Feline Hyperthyroidism

Exposure to flame retardant additives could be a contributing factor to the occurrence of feline hyperthyroidism (FH) in Swedish cats, according to a new study. The authors also suggest that pet cats can be studied as markers of environmental contamination in the home.

Webcasts

Pesticide Residue Analysis: Tips and Tricks for the Whole Workflow

PART III: Maximizing Analysis Efficiency through New GC-MS Approaches

Wednesday, June 17, 2015  — 8:00am PDT/ 11:00am EDT/ 4:00pm BST/ 5:00pm CEST

 

 

LCGC eBooks

Metabolomics: Prospects and Pitfalls

Given the complexity of biological systems, studying the metabolome is a challenging undertaking. Prof. Oliver Fiehn, PhD, Director of the NIH West Coast Metabolomics Center, University of California, Davis, explains steps to follow in the metabolomic workflow and addresses common metabolomic questions

Go to E-Book Library>>

 

CHROMacademy

Helium to Hydrogen — A Change Would Do You Good

Change is always a scary concept but let us dispel some of the fear associated with changing your helium carrier gas to hydrogen by answering some of the most common questions posed to us.

Reversed-Phase HPLC for the Analysis of Biomolecules

The following article from LCGC's ChromAcademy introduces the fundamentals of biopharmaceutical analysis and cover the use of reversed-phase HPLC in the analysis of biomolecules.

HPLC Troubleshooting Guide to Cycling Baselines and Pressure Fluctuations

HPLC troubleshooting guide to cycling baselines and pressure fluctuations from LCGC's ChromAcademy.

My LC–MS isn’t behaving! Where do I start?

Instrument manufacturers try to convince us that mass spec is just another detector. Most of us who work with LC-MS know that’s simply not the case – they can be maintenance intensive, unforgiving and generate complex information. When they’re not working it can be difficult to work out exactly where the problem lies. Here’s some advice to point you in the right direction

HPLC Column Dead Volume

A question about column dead volume. If the hold-up volume (time) is the extra-column dead volume expressed in time or volume. What is the column dead volume and how can it be calculated?