Liquid Chromatography

An Open-Source Simulator for Exploring HPLC Theory

By Daniel Abate-Pella, Dwight R. Stoll, Peter W. Carr, Paul G. Boswell

Despite the utility of high performance liquid chromatography (HPLC) simulators, we found that all the free and low-cost simulators were outdated or had extremely limited functionality, so we created one that addressed these shortcomings.

Choosing the Right HPLC Stationary Phase

By Tony Taylor

There is a bewildering array of stationary-phase choices available for reversed-phase high performance liquid chromatography (HPLC), and even within each phase designation (such as "C18") the selectivity of each phase can vary widely.

LC–MS-Compatible Approaches for the Quantitation of Limonin in Citrus Juice

By Joshua E. Young, Joseph J. Pesek, Maria T. Matyska

Limonin was separated from other components in citrus juice sample matrices by reversed-phase chromatography with ultraviolet detection.

Gas Chromatography

Extending the Compatible Analyte Volatility Range for Indoor Air Quality and Material Emissions Testing Using Multi-Bed Thermal Desorption Tubes

By Caroline Widdowson, David Barden

This article describes sampling methodology for thermal desorption–gas chromatography (TD–GC) that can extend the compatible analyte range of tests used to determine chemicals released from materials, as well as associated indoor air quality measurements.

GC Troubleshooting in Petrochemical Analysis

By Stephen Harrison

A guide to simple troubleshooting steps in gas chromatography (GC) with an emphasis on petrochemical analysis.

The Smell of Success: Using GC–MS–MS for Low Level Allergenic Fragrance Detection

By Gordon van ‘t Slot, Joe Anacleto

The regulatory and practical issues that surround allergenic fragrance use within cosmetics and cleaning products are explored in this article.

Mass Spectrometry

Quantifying Small Molecules by Mass Spectrometry

By P. Jane Gale, Alfred L. Yergey, Mark W. Duncan, Kate Yu

The use of a mass spectrometer in quantitative analysis exploits its exquisite selectivity and sensitivity as a detector, allowing a signal to be ascribed to a particular chemical entity with high certainty, even when present in a sample at a low concentration. There are, however, some special considerations that are necessary when a mass spectrometer is used as a quantitative tool.

Unraveling the Links Between Diet and Human Health Using LC–MS-MS

By LCGC Editors

We recently spoke to Gary Duncan and Wendy Russell of the Rowett Institute of Nutrition & Health in Aberdeen, Scotland, about the significance of phytochemical bioavailability to human health and the important role of liquid chromatography linked to tandem mass spectrometry (LC–MS-MS) in their research.

GC–MS Analysis of an Herbal Medicinal Remedy to Identify Potential Toxic Compounds

By Irene W. Kimaru, Hang P. Nguyen

In most countries, herbal medicinal products (HMPs) are introduced into the market without proper scientific evaluation or enforced safety and toxicological studies.

Sample Preparation Techniques

Simplifying Liquid Extractions

By Erica Pike

The benefits of supported liquid extraction (SLE) in sample cleanup and, in particular, the use of a synthetic SLE sorbent are discussed.

Pyrolysis for the Preparation of Macromolecules and Analysis by Gas Chromatography

By Douglas E. Raynie

When pyrolyzed, macromolecules will decompose into smaller fragments that can have the appropriate volatility for gas chromatography (GC) separation and analysis.

Understanding and Improving Solid-Phase Extraction

By Dawn Wallace Watson, Douglas E. Raynie

In this installment of "Sample Prep Perspectives," we cover some of the basic scientific principles behind solid-phase extraction (SPE) to allow the correct mode of extraction to be selected through an understanding of how analytes interact with and are separated by the sorbent.

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The LCGC Blog: Useful Tips for GC-MS

By Tony Taylor

There are many aspects of analytical science which abound with myth and legend – but gas chromatography – mass spectrometry (GC-MS), and more specifically the electron ionization (EI) process, stands out as the technique which has given rise to the largest number of ‘urban myths’ and misunderstandings.

The LCGC Blog: How to Get the Most Out of Your First Conference Experience

By Kevin A. Schug

In this blog installment, I would like to share a little advice with those of you who are attending your first major conference.

Latest News

Jaap Venema named Chief Science Officer of USP

The United Stated Pharmacopeial Convention (USP) has named Jaap Venema as the Chief Science Officer, beginning on April 15, and Chair of USP’s standards-setting body, the Council of Experts, at the beginning of USP’s next five-year cycle on July 1, 2015.

Caroline West, LCGC’s 2015 Emerging Leader Award Winner, Focuses on the Fundamentals of Selectivity

Caroline West, the winner of the 2015 LCGC Emerging Leader in Chromatography award, has a diverse set of scientific interests — in high-performance liquid chromatography (HPLC), supercritical fluid chromatography (SFC), hydrophilic-interaction chromatography (HILIC), and enantioselective separations — but all primarily focused on the fundamentals of chromatographic selectivity.

Detecting Off-Target Bromethalin Poisoning

Researchers from the California Animal Health and Food Safety Laboratory at the University of California Davis in the USA have developed an ultrahigh-performance liquid chromatography–mass spectrometry (UHPLC–MS) method for the detection of desmethylbromethalin (DMB) residues in animal tissues.

Analyzing PSTs in Shellfish Aquaculture

Paralytic shellfish toxins (PSTs) are naturally occurring toxins produced by some species of microscopic algae that can accumulate in filter feeding shellfish. These toxins are a threat to shellfish aquaculture and pose a serious hazard to public health when ingested. A group of scientists has developed a method using hydrophilic interaction liquid chromatography coupled to ultrahigh?performance LC tandem mass spectrometry (HILIC UHPLC–MS–MS) to determine PSTs in a variety of shellfish species as part of the Safe New Zealand Seafood Research programme.

Profiling PBDE Levels In Baltic Sea Fish

Fish from the Baltic Sea are a major source of lipophilic environmental pollutants for consumers in Finland. Surrounded by land, the Baltic Sea is one of the most threatened marine environments, making fish from the Baltic Sea a major source of lipophilic environmental pollutants including polybrominated diphenyl ethers (PBDEs).



Simplifying Carbohydrate Testing in Food and Beverages to Meet Food Quality and Labeling Requirements Using Ion Chromatography and Pulsed Amperometric Detection

Thursday, April 2, 2015 — 8:00 am PDT/ 11:00 am EDT/ 4:00 pm BST/ 5:00 pm CEST


Trouble-Free HPLC Analysis of Creams and Lotions

Tuesday, April 7, 2015 — 8:00 am PDT / 11:00 am EDT / 4:00 pm BST / 5:00 pm CEST


Pesticide Residue Analysis: Tips and Tricks for the Whole Workflow

PART II: Wednesday, April 29, 2015 — Workflow Guide for the use of LC-MS 8:00am PDT/ 11:00am EDT/ 4:00pm BST/ 5:00pm CEST


The Diversity of UV/Vis/NIR Techniques for Nanomaterial Characterization: How to use transmission, scatter transmission, diffuse reflectance, and angular resolved scattering measurements to characterize nano structures

Wednesday, April 29, 20158:00 am PDT/ 11:00 am EDT/ 4:00 pm BST/ 5:00 pm CEST


Achieve More With The Right Chromatography Data System

Thursday, April 30, 20159:00 am EDT/ 2:00 pm BST/ 3:00 pm CEST


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LCGC eBooks


Metabolomics: Prospects and Pitfalls

Given the complexity of biological systems, studying the metabolome is a challenging undertaking. Prof. Oliver Fiehn, PhD, Director of the NIH West Coast Metabolomics Center, University of California, Davis, explains steps to follow in the metabolomic workflow and addresses common metabolomic questions



HPLC Troubleshooting Guide to Cycling Baselines and Pressure Fluctuations

HPLC troubleshooting guide to cycling baselines and pressure fluctuations from LCGC's ChromAcademy.

My LC–MS isn’t behaving! Where do I start?

Instrument manufacturers try to convince us that mass spec is just another detector. Most of us who work with LC-MS know that’s simply not the case – they can be maintenance intensive, unforgiving and generate complex information. When they’re not working it can be difficult to work out exactly where the problem lies. Here’s some advice to point you in the right direction

HPLC Column Dead Volume

A question about column dead volume. If the hold-up volume (time) is the extra-column dead volume expressed in time or volume. What is the column dead volume and how can it be calculated?

Seven Must Know Points to Understand Atmospheric Pressure Chemical Ionization

CHROMacademy delivers key knowledge quickly in bite-size modules, and to prove this here is our seven point guide to understanding atmospheric pressure chemical ionization.

HPLC Extra Column Volumes

It is of particular importance to reduce extra column volumes when using small volume columns or UHPLC. However, where do these extra column volumes come from, how can they be minimized, and what effect do they have on chromatography?