Liquid Chromatography

Retention and Selectivity of Stationary Phases Used in HILIC

By David S. Bell

Understanding the interactions that stationary-phase chemistries provide will help you choose the most appropriate blend of interactions to address a given separation challenge.

Large-Volume Injection LC–MS-MS Methods for Aqueous Samples and Organic Extracts

By B. McKay Allred, Matthew J. Perkins, Jennifer A. Field

Environmental sample analysis by large-volume injection (LVI) in combination with liquid chromatography–tandem mass spectrometry (LC–MS-MS) is described for polar and nonpolar analytes in both aqueous samples and organic extracts.

Seven Things to Avoid in the Liquid Chromatography Laboratory

By John W. Dolan

Have a problem with your LC system? Maybe applying a little "DDT (Don't Do That)" may help.

Gas Chromatography

Pragmatic Rules for GC Column Selection

By Tony Taylor

Choosing the correct column selectivity and dimensions is fundamental to successful gas chromatography method development.

GC Detectors: From Thermal Conductivity to Vacuum Ultraviolet Absorption

By Kevin A. Schug, Harold M. McNair

Some might consider gas chromatography (GC) a mature technique. However, several substantial advances in GC technology in the past few years have proven that there is still room for innovation.

Critical Evaluation of GC Methods

By Tony Taylor

How to spot weaknesses in methods before problems occur

Mass Spectrometry

Unraveling the Links Between Diet and Human Health Using LC–MS-MS

By LCGC Editors

We recently spoke to Gary Duncan and Wendy Russell of the Rowett Institute of Nutrition & Health in Aberdeen, Scotland, about the significance of phytochemical bioavailability to human health and the important role of liquid chromatography linked to tandem mass spectrometry (LC–MS-MS) in their research.

GC–MS Analysis of an Herbal Medicinal Remedy to Identify Potential Toxic Compounds

By Irene W. Kimaru, Hang P. Nguyen

In most countries, herbal medicinal products (HMPs) are introduced into the market without proper scientific evaluation or enforced safety and toxicological studies.

Simultaneous Determination of Methylxanthines and Cotinine in Human Plasma by Solid-Phase Extraction Followed by LC–MS-MS

By Rossana Bossi, Bodil Hammer Bech

A multiresidue method has been developed and validated for the analysis of methylxanthines (caffeine and its metabolites) and cotinine in human plasma.

Sample Preparation Techniques

Pyrolysis for the Preparation of Macromolecules and Analysis by Gas Chromatography

By Douglas E. Raynie

When pyrolyzed, macromolecules will decompose into smaller fragments that can have the appropriate volatility for gas chromatography (GC) separation and analysis.

Understanding and Improving Solid-Phase Extraction

By Douglas E. Raynie, Dawn Wallace Watson

In this installment of "Sample Prep Perspectives," we cover some of the basic scientific principles behind solid-phase extraction (SPE) to allow the correct mode of extraction to be selected through an understanding of how analytes interact with and are separated by the sorbent.

Developments in Green Chromatography

By Marek Tobiszewski, Jacek Namiesnik

Green analytical chemistry is a widely recognized concept that has led to the development of new analytical methods with reduced environmental impact and minimized analyst occupational exposure. This article presents the most recent progress in the development of greener sample preparation and chromatographic separation techniques.

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LCGC Blog

HPLC Column Transfer from Fully Porous to Core-Shell Particles in Three Simple Equations

By Tony Taylor

There are often times in my work when I need to 'mess about' with column dimensions and particle morphologies. For 'mess about' read improve or transfer.

Five Steps in the Evolution of an Instrumental Analysis Course for Enhanced Student Preparation

By Kevin A. Schug

I have been at The University of Texas at Arlington for almost 10 years, and I have taught approximately seven different classes in my time here. Yet, the one that I have had the most opportunities to teach, and the most time to reform, is our junior/senior-level Instrumental Analysis (IA) course.

Latest News

Improving LC–MS–MS Determination of Trace Estrogens in Serum

Detecting estrogens at trace concentrations in biological fluids is key to understanding their role in the induction — and treatment — of disease. Researchers from the University of Texas Arlington, USA, have developed a method using restricted access-media (RAM) that enables the detection of trace levels of oestrogen's using LC–MS–MS without liquid–liquid extraction (LLE) or solid-phase extraction (SPE).

Investigating Insect Communication with HPLC-ELSD

Scientists have published a novel method using reversed-phase high performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) for the isolation and determination of nonvolatile methyl-branched hydrocarbons (MBCHs) from insect cuticles

Supplements Industry Plans Further Testing of Products Branded as Fraudulent

In response to recent accusations levied by the New York State Attorney General’s office that herbal supplements sold by four major retailers contained little or no primary ingredient, the supplements industry is planning further testing of the affected products.

Analyzing Swimming Pool Water

Pharmaceutical and personal care products (PPCPs) entering water supplies have been widely studied, but there is little information on the activity of these compounds in swimming pool water. Researchers have applied a PPCP analysis method previously developed for the detection of 32 PPCPs in wastewater to survey indoor swimming pools in the USA.

NY Attorney General’s Analysis of “Fraudulent” Herbal Supplements Was Based on DNA Testing, Not Chromatography

On Monday, February 2, the New York State Attorney General’s office sent letters to four major retailers accusing them of selling fraudulent herbal supplements and demanding that they remove the products from their shelves. To the surprise of many analytical chemists, however, the accusations were based on DNA barcoding technology rather than chromatographic analysis.

Webcasts

Use of Novel Zirconia-Based Cleanup Sorbents for the Analysis of Contaminants in Fatty Food Samples

Tuesday, March 31, 2015 — 8:00 am PDT/ 11:00 am EDT/ 4:00 pm GMT/ 5:00 pm CET

 

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LCGC eBooks

Featured: Applications of Ion Chromatography

You know the classic uses of ion chromatography (IC), like measuring inorganic ions in drinking water. But do you know what else it can do?

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CHROMacademy

My LC–MS isn’t behaving! Where do I start?

Instrument manufacturers try to convince us that mass spec is just another detector. Most of us who work with LC-MS know that’s simply not the case – they can be maintenance intensive, unforgiving and generate complex information. When they’re not working it can be difficult to work out exactly where the problem lies. Here’s some advice to point you in the right direction

HPLC Column Dead Volume

A question about column dead volume. If the hold-up volume (time) is the extra-column dead volume expressed in time or volume. What is the column dead volume and how can it be calculated?

Seven Must Know Points to Understand Atmospheric Pressure Chemical Ionization

CHROMacademy delivers key knowledge quickly in bite-size modules, and to prove this here is our seven point guide to understanding atmospheric pressure chemical ionization.

HPLC Extra Column Volumes

It is of particular importance to reduce extra column volumes when using small volume columns or UHPLC. However, where do these extra column volumes come from, how can they be minimized, and what effect do they have on chromatography?