Fundamental Questions

As you hurry through your daily work in the analytical laboratory, are you failing to ask yourself some fundamental questions about the methods you use and the results you produce?

This collection of short articles from LCGC’s digital magazine, The Column, raises four key questions you should ask. And if the answers aren’t what you hoped, we offer advice for addressing the gaps.

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Featured LCGC Interviews

Investigating Baby Food Using HILIC–MS/MS

Babies and infants experience rapid growth within a short timeframe and the nutrition that they absorb is therefore of the utmost importance. María Mateos-Vivas from the Department of Analytical Chemistry, Nutrition, and Food Science at the University of Salamanca, Spain, has used hydrophilic interaction liquid chromatography (HILIC) coupled to tandem mass spectrometry (MS/MS) to investigate the role of nucleotides on infant health. She recently spoke to us about this research.

Innovating Microfluidic Technologies

The future of biological and clinical research will depend on technological innovations and cross discipline co-operation as science seeks a deeper understanding of increasingly complex biological systems. The 2016 recipient of the AES Mid-Career Award, Amy Herr, and her team at the University of California Berkeley have explored these areas using a combination of chemical engineering, mechanical engineering, and electrical engineering with strong foundations in biology, material science, and analytical chemistry to innovate new microfluidic analytical technology. She recently spoke to LCGC about this work.

Gas Chromatography

Sealing it with Septa

By John V. Hinshaw

Choosing the wrong septa can compromise method accuracy.

Coupling Gas Chromatography with Ion Mobility Spectrometry

By Zygfryd Witkiewicz, Urszula Perycz, Mirosław Maziejuk, Jarosław Puton

Chromatography connected with ion mobility spectrometry (IMS) is not commonly used, but is being investigated more. IMS is an independent analytical technique with very good detectability and a rather small separation ability. One favourable property of IMS is that it can work with ambient pressure and can be easily connected to a gas chromatograph. Analytical applications of GC–MS are very different and encompass investigations into food, medical science, environment, drugs of abuse, chemical warfare agents, and explosives.

Mass Spectrometry

Urine Analysis: The Good, the Bad, and the Ugly

By Jenny Cybulski

In clinical and forensic/toxicology laboratories, urine is a preferred matrix from which to quantify drug concentrations because it yields accurate results and allows for noninvasive collection methods. Prior to excretion, drug metabolites in the body undergo a glucuronidation reaction, resulting in a glucuronide bond that must be cleaved before mass spectrometry (MS) analysis by a β-glucuronidase enzyme hydrolysis. Many laboratories employ a “dilute-and-shoot” method after hydrolysis to decrease residual protein or enzyme concentration, but this method negatively affects column lifetime and reduces the sensitivity of analyte detection. By using a β-glucuronidase removal approach, analysts are able to see an increase in sensitivity and a reduction in MS instrument maintenance.

Simultaneous Quantitation of Buprenorphine and Its Metabolites Using LC–MS

By Anand A. Joshi, Neha V. Maharao, Phillip M. Gerk

An LC–MS method for simultaneous quantification of buprenorphine and three metabolites: norbuprenorphine, buprenorphine glucuronide, and norbuprenorphine glucuronide.

Sample Preparation

New Sample Preparation Products and Accessories for 2016

By Douglas E. Raynie

Many of this year’s new products fit into recently identified trends, but one major driver of advances was not in our forecasts.

Trends in Sample Preparation

By Douglas E. Raynie

A comparison of the results from a new survey on sample preparation techniques to those of previous surveys, from 1991 to 2013, reveals some interesting trends.

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The LCGC Blog: Proper GC Column Installation – The Simplest Way to Improve Your Gas Chromatography

By Tony Taylor

The title of this piece may have put you off reading it – in which case you won’t be insulted when I say that even the most experienced gas chromatographers often fail to install columns in the best way possible.

Mass Spectrometry-Based Bacterial Identification in the Environment

By Kevin A. Schug

Matrix-assisted laser desorption–ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is actually one of the most versatile tools for fingerprinting mixtures.


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LCGC Slideshows

Slideshow: Seven Common Faux Pas in Modern HPLC

Seven outdated traditional practices that should not be performed without considering alternative approaches that can improve results, provide lower operation costs, or give faster run times. Instead of working harder, analytical scientists should work smarter. Learn more by clicking through the slideshow.

Slideshow: The Top 10 HPLC and UHPLC Column Myths

Webster’s New Collegiate Dictionary defines a myth as “an ill-founded belief held uncritically, especially by an interested group.” Could that group be misinformed chromatographers?

Latest News

The University of Manchester Opens Stoller Biomarker Discovery Centre

The University of Manchester unveiled the £18-million Stoller Biomarker Discovery Centre at the beginning of June 2016. The research centre will focus on biomedical research on major diseases, including cancer, psoriasis, and arthritis, using mass spectrometry (MS)‑based proteomics solutions from Sciex.

Researchers Use Chip CE to Investigate Rhinovirus Replication

Researchers from Vienna University of Technology (TU Wien), Austria, have used chip capillary electrophoresis (CE) in combination with molecular beacons (MBs) to analyze the release of the RNA genome from a human rhinovirus.

LCGC E-Books

Advancing the Analysis of Pesticides and Other Chemical Residues

The presence of pesticides and other chemical contaminants in food and dietary supplements is an important concern for both health and environmental reasons. In this new e-book, developed in collaboration with the organizers of the North American Chemical Residues Workshop (NACRW), four leading experts in the field, all frequent speakers at the conference, share some of their latest work and findings.

Go to E-Book Library>>



Is the Art of HPLC Method Development Dead?

CHROMacademy's Tutor Scott Fletcher discusses whether HPLC method development has become a dying art.

How to Optimize the Key Variables in GC Analysis: GC Columns and Detectors

Selecting a gas chromatography (GC) column can be a daunting task. It may seem like there are a never-ending number of phase chemistries, or an inordinate number of column geometry options. However, when choosing a column for a new application (or to improve an existing one) there are some simple rules that can be followed.

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