Featured LCGC Interviews

Back to Nature with Flash Chromatography

Dr Vera Thoss from Bangor University (Bangor, Wales) spoke to Bethany Degg of The Column about her work investigating the chemistry of plants and the value of flash chromatography in this area of research.

Liquid Chromatography

Food Allergen Analysis Using LC–MS–MS

By Ashley Sage

Changes to the European Food Safety Authority (EFSA) regulations on food labelling, and the strengths and limitations of new and existing methods for the detection of allergens in food are discussed in this article.

Fast and Simple Determination of Free Amino Acids in Beer

By Gesa J. Schad and Brigitte Bollig

A rapid and reliable high performance liquid chromatography–mass spectrometry (HPLC–MS) method to determine amino acids in beer is presented.

New Chromatography Columns and Accessories for 2015

By M. Swartz

This is our annual review of new liquid chromatography (LC) columns and accessories introduced at Pittcon and throughout the previous year.

Gas Chromatography

Metabolomics in Natural Product Analysis

By LCGC Editors

Metabolomics in Natural Product Analysis

Analysis of Shipwrecked Beer

By LCGC Editors

Analysis of Shipwrecked Beer

Understanding Electron Ionization Processes for GC–MS

By LCGC Editors

Many of us use electron ionization (EI) in gas chromatography–mass spectrometry (GC–MS) without a good understanding of the technique and how we might manipulate the process to give more appropriate results or a better understanding of the analytes under investigation.

Mass Spectrometry

Metabolomics in Natural Product Analysis

By LCGC Editors

Metabolomics in Natural Product Analysis

Analysis of Shipwrecked Beer

By LCGC Editors

Analysis of Shipwrecked Beer

Quantifying Small Molecules by Mass Spectrometry

By P. Jane Gale, Alfred L. Yergey, Mark W. Duncan, Kate Yu

The use of a mass spectrometer in quantitative analysis exploits its exquisite selectivity and sensitivity as a detector, allowing a signal to be ascribed to a particular chemical entity with high certainty, even when present in a sample at a low concentration. There are, however, some special considerations that are necessary when a mass spectrometer is used as a quantitative tool.

Sample Preparation Techniques

Solid Phase Extraction for the Analysis of Drugs of Abuse in Urine

By FMS, Inc.

The term Drugs of Abuse (DOA) can be applied to a wide variety of natural and synthetic compounds prevalent in human culture. Many rapidly pass through the human body, while others can persist for extended periods. Screening for traces of these has become common place by analyzing human urine, either by LC/MS or by GC/MS through derivitization of the parent compounds. Solid Phase Extraction (SPE) provides an ideal extraction media for DOA compounds, as it can be performed with small sample amounts with rapid turnaround.

Solid Phase Extraction for the Analysis of Drugs of Abuse in Urine

By FMS, Inc.

The term Drugs of Abuse (DOA) can be applied to a wide variety of natural and synthetic compounds prevalent in human culture. Many rapidly pass through the human body, while others can persist for extended periods. Screening for traces of these has become common place by analyzing human urine, either by LC/MS or by GC/MS through derivitization of the parent compounds. Solid Phase Extraction (SPE) provides an ideal extraction media for DOA compounds, as it can be performed with small sample amounts with rapid turnaround.

Fast GC–TOF MS for high-throughput screening of environmental contaminants

By Markes International

This poster, presented at Pittcon 2015, describes how fast gas GC with time-of-flight mass spectrometry (TOF MS) can allow identification of both target environmental pollutants and unknowns in a single, rapid analysis. Particular attention is given to the reproducibility of quantitation and qualifier ion peak areas, and the ability to generate spectra closely matching those in the NIST library.

Partner Organizations

 

Sepu, China's Largest Chromatography Training Site

About Sepu

News from Sepu

 

Chinese American Chromatography Association (CACA)

About CACA

 

ChromAcademy

ChromAcademy Homepage

LCGC Blog

The LCGC Blog: Four Steps to Troubleshooting an HPLC Problem

By Tony Taylor

What if we could make the troubleshooting poster on the laboratory wall come to life? What if we could build an engine which figured out the most likely causes of groups of symptoms and offer these up as a prioritised list for folks to work through and give them supporting information on each problem, why it occurred, how to fix it and, crucially, how to avoid it happening next time?

A New Consortium of Researchers for Environmental Analysis and Remediation

By Kevin A. Schug

CLEAR is an endeavor to create a consortium of experts interested in building and propagating activities related to the assessment of the potential impact of industrial activities on the environment, as well as the creation of new solutions to mitigate problems associated with these activities.

Latest News

Sustainable Reuse of Fracking Water

Researchers from the University of Colorado have characterized flow?back water generated from fracking activities, using a combination of analytical techniques, to determine how best to treat wastewater so that it can be recycled.

Using Proteomics to Determine Key to Embryo Implantation Success

A new study presents a proteomics approach to investigate changes at the molecular scale that could control whether or not embyro implantation using IVF is successful.

Dietary Supplements Tainted with Amphetamine Isomer BMPEA

Selected dietary supplements labelled to contain Acacia rigidula have been found to be adulterated with the amphetamine isomer ?-methylphenylethylamine (BMPEA).

GC–MS Detects Chemical Cue for Mosquitoes in Soil

Cedrol, a sequisterpene alcohol found in the essential oil of connifers, could be a potent chemical cue for pregnant mosquitoes seeking the ideal location to lay their eggs.

Lemur Olfactory Cues

Scientists at Duke University in Durham, North Carolina, USA, have discovered that olfactory cues can indicate pregnancy and foetal sex in the ring-tailed lemur (Lemur catta). Using gas chromatography coupled to mass spectrometry (GC–MS), the team found that lemurs carrying male offspring exhibited a different volatile chemical profile to those carrying a female.

Webcasts

Pesticide Residue Analysis: Tips and Tricks for the Whole Workflow

PART II: Wednesday, April 29, 2015 — Workflow Guide for the use of LC-MS — 8:00am PDT/ 11:00am EDT/ 4:00pm BST/ 5:00pm CEST

 

The Diversity of UV/Vis/NIR Techniques for Nanomaterial Characterization: How to use transmission, scatter transmission, diffuse reflectance, and angular resolved scattering measurements to characterize nano structures

Wednesday, April 29, 2015 —8:00 am PDT/ 11:00 am EDT/ 4:00 pm BST/ 5:00 pm CEST

 

Achieve More With The Right Chromatography Data System

Thursday, April 30, 2015 — 9:00 am EDT/ 2:00 pm BST/ 3:00 pm CEST

 

HRMS in Clinical Research: From Targeted Quantification to Metabolomics

Thursday, May 7, 2015 — 8:00 am PDT/ 11:00 am EDT/ 4:00 pm BST/ 5:00 pm CET

 

More Upcoming & On Demand Webcasts>>

LCGC eBooks

Metabolomics: Prospects and Pitfalls

Given the complexity of biological systems, studying the metabolome is a challenging undertaking. Prof. Oliver Fiehn, PhD, Director of the NIH West Coast Metabolomics Center, University of California, Davis, explains steps to follow in the metabolomic workflow and addresses common metabolomic questions

Go to E-Book Library>>

 

CHROMacademy

Reversed-Phase HPLC for the Analysis of Biomolecules

The following article from LCGC's ChromAcademy introduces the fundamentals of biopharmaceutical analysis and cover the use of reversed-phase HPLC in the analysis of biomolecules.

HPLC Troubleshooting Guide to Cycling Baselines and Pressure Fluctuations

HPLC troubleshooting guide to cycling baselines and pressure fluctuations from LCGC's ChromAcademy.

My LC–MS isn’t behaving! Where do I start?

Instrument manufacturers try to convince us that mass spec is just another detector. Most of us who work with LC-MS know that’s simply not the case – they can be maintenance intensive, unforgiving and generate complex information. When they’re not working it can be difficult to work out exactly where the problem lies. Here’s some advice to point you in the right direction

HPLC Column Dead Volume

A question about column dead volume. If the hold-up volume (time) is the extra-column dead volume expressed in time or volume. What is the column dead volume and how can it be calculated?

Seven Must Know Points to Understand Atmospheric Pressure Chemical Ionization

CHROMacademy delivers key knowledge quickly in bite-size modules, and to prove this here is our seven point guide to understanding atmospheric pressure chemical ionization.