Featured LCGC Interviews

Emerging Trends in Pharmaceutical Analysis

Benedetto Natalini of the University of Perugia, Italy, spoke to The Column about the driving forces in pharmaceutical analysis, including the importance of regulation, chirality, and miniaturization.

Liquid Chromatography

Food Allergen Analysis Using LC–MS–MS

By Ashley Sage

Changes to the European Food Safety Authority (EFSA) regulations on food labelling, and the strengths and limitations of new and existing methods for the detection of allergens in food are discussed in this article.

Fast and Simple Determination of Free Amino Acids in Beer

By Gesa J. Schad and Brigitte Bollig

A rapid and reliable high performance liquid chromatography–mass spectrometry (HPLC–MS) method to determine amino acids in beer is presented.

New Chromatography Columns and Accessories for 2015

By M. Swartz

This is our annual review of new liquid chromatography (LC) columns and accessories introduced at Pittcon and throughout the previous year.

Gas Chromatography

Understanding Electron Ionization Processes for GC–MS

By LCGC Editors

Many of us use electron ionization (EI) in gas chromatography–mass spectrometry (GC–MS) without a good understanding of the technique and how we might manipulate the process to give more appropriate results or a better understanding of the analytes under investigation.

Extending the Compatible Analyte Volatility Range for Indoor Air Quality and Material Emissions Testing Using Multi-Bed Thermal Desorption Tubes

By Caroline Widdowson, David Barden

This article describes sampling methodology for thermal desorption–gas chromatography (TD–GC) that can extend the compatible analyte range of tests used to determine chemicals released from materials, as well as associated indoor air quality measurements.

GC Troubleshooting in Petrochemical Analysis

By Stephen Harrison

A guide to simple troubleshooting steps in gas chromatography (GC) with an emphasis on petrochemical analysis.

Mass Spectrometry

Quantifying Small Molecules by Mass Spectrometry

By P. Jane Gale, Alfred L. Yergey, Mark W. Duncan, Kate Yu

The use of a mass spectrometer in quantitative analysis exploits its exquisite selectivity and sensitivity as a detector, allowing a signal to be ascribed to a particular chemical entity with high certainty, even when present in a sample at a low concentration. There are, however, some special considerations that are necessary when a mass spectrometer is used as a quantitative tool.

Unraveling the Links Between Diet and Human Health Using LC–MS-MS

By LCGC Editors

We recently spoke to Gary Duncan and Wendy Russell of the Rowett Institute of Nutrition & Health in Aberdeen, Scotland, about the significance of phytochemical bioavailability to human health and the important role of liquid chromatography linked to tandem mass spectrometry (LC–MS-MS) in their research.

GC–MS Analysis of an Herbal Medicinal Remedy to Identify Potential Toxic Compounds

By Irene W. Kimaru, Hang P. Nguyen

In most countries, herbal medicinal products (HMPs) are introduced into the market without proper scientific evaluation or enforced safety and toxicological studies.

Sample Preparation Techniques

Determination of Organophosphate Pesticides in Urine

By UCT Inc.

Organophosphate Pesticides (OP) are a diverse group of compounds. Derived from Phosphoric acid they exhibit varied physicochemical properties. They are used extensively as nerve poisons to kill target pests (usually insects). However, their toxicity extends to mammals and they can adversely affect the human nervous system, even at low exposure levels.

Determination of Organophosphate Pesticides in Urine

By UCT Inc.

Organophosphate Pesticides (OP) are a diverse group of compounds. Derived from Phosphoric acid they exhibit varied physicochemical properties. They are used extensively as nerve poisons to kill target pests (usually insects). However, their toxicity extends to mammals and they can adversely affect the human nervous system, even at low exposure levels.

Simplifying Liquid Extractions

By Erica Pike

The benefits of supported liquid extraction (SLE) in sample cleanup and, in particular, the use of a synthetic SLE sorbent are discussed.

Partner Organizations

 

Sepu, China's Largest Chromatography Training Site

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Chinese American Chromatography Association (CACA)

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ChromAcademy

ChromAcademy Homepage

LCGC Blog

The LCGC Blog: Four Steps to Troubleshooting an HPLC Problem

By Tony Taylor

What if we could make the troubleshooting poster on the laboratory wall come to life? What if we could build an engine which figured out the most likely causes of groups of symptoms and offer these up as a prioritised list for folks to work through and give them supporting information on each problem, why it occurred, how to fix it and, crucially, how to avoid it happening next time?

A New Consortium of Researchers for Environmental Analysis and Remediation

By Kevin A. Schug

CLEAR is an endeavor to create a consortium of experts interested in building and propagating activities related to the assessment of the potential impact of industrial activities on the environment, as well as the creation of new solutions to mitigate problems associated with these activities.

Latest News

GC–MS Detects Chemical Cue for Mosquitoes in Soil

Cedrol, a sequisterpene alcohol found in the essential oil of connifers, could be a potent chemical cue for pregnant mosquitoes seeking the ideal location to lay their eggs.

Lemur Olfactory Cues

Scientists at Duke University in Durham, North Carolina, USA, have discovered that olfactory cues can indicate pregnancy and foetal sex in the ring-tailed lemur (Lemur catta). Using gas chromatography coupled to mass spectrometry (GC–MS), the team found that lemurs carrying male offspring exhibited a different volatile chemical profile to those carrying a female.

Analyzing POPs in 50 µL of Blood Samples

Scientists from SGC Environmental Services in Wilmington, North Carolina, USA, have demonstrated the development and validation of a method for the analysis of five persistent organic pollutants (POPs) from 50 µL dried blood spots. The work was presented as a poster at Dioxin 2014, held in Madrid, Spain, from 31 August to 5 September.

Jaap Venema named Chief Science Officer of USP

The United Stated Pharmacopeial Convention (USP) has named Jaap Venema as the Chief Science Officer, beginning on April 15, and Chair of USP’s standards-setting body, the Council of Experts, at the beginning of USP’s next five-year cycle on July 1, 2015.

Caroline West, LCGC’s 2015 Emerging Leader Award Winner, Focuses on the Fundamentals of Selectivity

Caroline West, the winner of the 2015 LCGC Emerging Leader in Chromatography award, has a diverse set of scientific interests — in high-performance liquid chromatography (HPLC), supercritical fluid chromatography (SFC), hydrophilic-interaction chromatography (HILIC), and enantioselective separations — but all primarily focused on the fundamentals of chromatographic selectivity.

Webcasts

Pesticide Residue Analysis: Tips and Tricks for the Whole Workflow

PART II: Wednesday, April 29, 2015 — Workflow Guide for the use of LC-MS — 8:00am PDT/ 11:00am EDT/ 4:00pm BST/ 5:00pm CEST

 

The Diversity of UV/Vis/NIR Techniques for Nanomaterial Characterization: How to use transmission, scatter transmission, diffuse reflectance, and angular resolved scattering measurements to characterize nano structures

Wednesday, April 29, 2015 —8:00 am PDT/ 11:00 am EDT/ 4:00 pm BST/ 5:00 pm CEST

 

Achieve More With The Right Chromatography Data System

Thursday, April 30, 2015 — 9:00 am EDT/ 2:00 pm BST/ 3:00 pm CEST

 

HRMS in Clinical Research: From Targeted Quantification to Metabolomics

Thursday, May 7, 2015 — 8:00 am PDT/ 11:00 am EDT/ 4:00 pm BST/ 5:00 pm CET

 

More Upcoming & On Demand Webcasts>>

LCGC eBooks

 

Metabolomics: Prospects and Pitfalls

Given the complexity of biological systems, studying the metabolome is a challenging undertaking. Prof. Oliver Fiehn, PhD, Director of the NIH West Coast Metabolomics Center, University of California, Davis, explains steps to follow in the metabolomic workflow and addresses common metabolomic questions

Go to E-Book Library

CHROMacademy

HPLC Troubleshooting Guide to Cycling Baselines and Pressure Fluctuations

HPLC troubleshooting guide to cycling baselines and pressure fluctuations from LCGC's ChromAcademy.

My LC–MS isn’t behaving! Where do I start?

Instrument manufacturers try to convince us that mass spec is just another detector. Most of us who work with LC-MS know that’s simply not the case – they can be maintenance intensive, unforgiving and generate complex information. When they’re not working it can be difficult to work out exactly where the problem lies. Here’s some advice to point you in the right direction

HPLC Column Dead Volume

A question about column dead volume. If the hold-up volume (time) is the extra-column dead volume expressed in time or volume. What is the column dead volume and how can it be calculated?

Seven Must Know Points to Understand Atmospheric Pressure Chemical Ionization

CHROMacademy delivers key knowledge quickly in bite-size modules, and to prove this here is our seven point guide to understanding atmospheric pressure chemical ionization.

HPLC Extra Column Volumes

It is of particular importance to reduce extra column volumes when using small volume columns or UHPLC. However, where do these extra column volumes come from, how can they be minimized, and what effect do they have on chromatography?