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LCGC TV Video: Is 2D LC Ready for Prime Time Analysis?: 2D LC has huge potential to increase the resolving power of separations involving complex samples. In this new video clip, Dwight Stoll of Gustavus Adolphus College considers if this technique is ready to be adopted by chromatographers working in industry.

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LCGC COLUMNISTS 2014

Column Watch: Ron Majors, established authority on new column technologies, keeps readers up-to-date with new sample preparation trends in all branches of chromatography and reviews developments. LATEST: Avoiding Reversed-Phase Chromatography Problems Through Informed Method Development Practices: Choosing the Stationary-Phase Chemistry


Perspectives in Modern HPLC: Michael W. Dong is a senior scientist in Small Molecule Drug Discovery at Genentech in South San Francisco, California. He is responsible for new technologies, automation, and supporting late-stage research projects in small molecule analytical chemistry and QC of small molecule pharmaceutical sciences. LATEST: Seven Common Faux Pas in Modern HPLC


MS — The Practical Art: Kate Yu brings her expertise in the field of mass spectrometry and hyphenated techniques to the pages of LCGC. In this column she examines the mass spectrometric side of coupled liquid and gas-phase systems. Troubleshooting-style articles provide readers with invaluable advice for getting the most from their mass spectrometers. LATEST: Radical Mass Spectrometry as a New Frontier for Bioanalysis


LC Troubleshooting: LC Troubleshooting sets about making HPLC methods easier to master. By covering the basics of liquid chromatography separations and instrumentation, John Dolan is able to highlight common problems and provide remedies for them. LATEST: Estimating Resolution for Marginally Separated Peaks


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Features
Avoiding Reversed-Phase Chromatography Problems Through Informed Method Development Practices: Choosing the Stationary-Phase Chemistry
Understanding the chemistry of embedded polar group and perfluorophenyl phases leads to better decisions during method developement
Optimizing UHPLC Methods
In this new video from LCGC TV, Richard Henry, Consultant gives his advice on how to select UHPLC column size and discusses the features of an optimal UHPLC instrument.
Recent Developments in Ion Mobility Mass Spectrometry for Bioanalysis
This article reviews recent developments that aim to address the conflicting demands for higher throughput, sensitivity, and selectivity in bioanalysis applications.
The Role of Elution Gradient Shape in the Separation of Protein Therapeutics
A discussion of the role of the gradient in separation by reversed-phase and ion-exchange HPLC, with data from two products to illustrate the key points
Quantifying Proteins by Mass Spectrometry
A review of the essential elements of MS approaches to protein quantification and a critical comparison of the available options

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