In developing countries, access to medicine is often limited. To make matters worse, widespread counterfeiting often means that even when patients get access to drugs, the drugs are of poor quality. Dr. Mélisande Bernard, of the Agence Générale des Equipements et Produits de Santé, the technical and pharmaceutical service of the public hospital system of Paris, France (Assistance Publique–Hôpitaux de Paris), is helping to address this problem by developing chromatographic methods to detect substandard cardiovascular drugs in Africa. She recently spoke to us about this work.
A selection of commonly asked questions about GPC/SEC from users.
Solid-phase microextraction (SPME) coupled with comprehensive two?dimensional gas chromatography and time-of-flight mass spectrometry (GC×GC–TOF-MS) can be used to detect trace-level fungicides and compounds responsible for undesirable attributes known as “organoleptic faults” in wine. This article explains more.
The main objective of this study was to evaluate the capabilities of gas chromatography (GC) with time-of-flight mass spectrometry (MS) for screening pesticides in fruits and vegetables using a purpose-built accurate-mass database.
The development of a novel trapping system and a modified GC–MS layout (using dual chromatographic columns and cryogenic focusing devices) has enabled a major improvement in the chromatographic separation of volatile and semivolatile compounds in cigarette smoke. This improvement has led to the potential for identifying compounds which are usually masked by the solvent peak.
We have developed a miniaturized, multifunctional device, called the "Single-Probe," that is capable of probing small targets and of sampling and ionizing molecular species.
An important attribute of a novel ionization process for use in mass spectrometry (MS) is its simplicity and flexibility to be hyphenated to conventional liquid-based separation methods.
The use of a mass spectrometer in quantitative analysis exploits its exquisite selectivity and sensitivity as a detector, allowing a signal to be ascribed to a particular chemical entity with high certainty, even when present in a sample at a low concentration. There are, however, some special considerations that are necessary when a mass spectrometer is used as a quantitative tool.
Volumetric absorptive microsampling (VAMS) is gaining traction because it delivers the benefits of dried blood spots (DBS) and overcomes its limitations while generating comparable PK data to conventional sampling methods. This article explains more.
When pyrolyzed, macromolecules will decompose into smaller fragments that can have the appropriate volatility for gas chromatography (GC) separation and analysis.
By Tony Taylor
Once you have mastered the terminology and symbology the actual mathematics for the models and approaches used at the level which is useful to practicing analytical chemists is really very straightforward indeed.
For the analytical community, method validation in some form or another is a natural extension of best practice in the analytical laboratory. However, the notion of method validation, and many aspects of detailed forensics analysis, are not well understood by most lawyers and judges.
Methadone, a drug prescribed for managing the symptoms of heroin withdrawal and chronic pain, has been linked to the formation of the carcinogenic compound N-Nitrosodimethylamine (NDMA) in drinking water. Understanding which compounds contribute to NDMA formation in water could lead to tailored wastewater treatment approaches to remove the risk of contamination.
Scientists have published a novel method to detect multiple celiac disease-epitopes in wheat extracts simultaneously using liquid chromatography–multiple reaction monitoring mass spectrometry (LC–MRM–MS).
A new study hopes to highlight that poison was used as far back as 30,000 years ago. Dr Valentina Borgia, a specialist in Palaeolithic hunting weapons and Marie Curie Fellow at the McDonald Institute for Archaeological Research at the University of Cambridge, is using liquid chromatography combined with mass spectrometry (LC–MS) to analyze prehistoric weaponry.
LCGC Magazine is pleased to announce the addition of Taylor Zhang to its editorial advisory board.
Parabens are commonly used in cosmetics and personal care products to protect against microbial growth, but a new study suggests that manufacturers may also be using parabens in plastic teething products designed for infants.
MAb Charge Heterogeneity Analysis by CZE Series: Part 3: A Test Method Fit for QC Testing
Thursday, July 2, 2015 — 7 am PDT/ 10 am EDT/ 4 pm CEST
Latest Developments & Future Directions in Data Processing & Analysis Software for LC-MS-MS & GC-MS-MS
Part 4: Wednesday, 15 July 2015 — 8 am PDT, 11 am EDT, 4 pm BST, 5 pm CEST
Editors’ Series: Part 2: Application of Two-Dimensional Liquid Chromatography in Pharmaceutical Analysis
Tuesday, July 28, 2015 — 10 am EDT/ 3 pm BST/ 4 pm CEST
To characterize biopharmaceuticals, particularly monoclonal antibodies and antibody–drug conjugates (ADCs), you need a complete toolbox of powerful tools. You are probably familiar with LC–MS methods. But have you seen what light-scattering detection can do?
Change is always a scary concept but let us dispel some of the fear associated with changing your helium carrier gas to hydrogen by answering some of the most common questions posed to us.
The following article from LCGC's ChromAcademy introduces the fundamentals of biopharmaceutical analysis and cover the use of reversed-phase HPLC in the analysis of biomolecules.
HPLC troubleshooting guide to cycling baselines and pressure fluctuations from LCGC's ChromAcademy.
Instrument manufacturers try to convince us that mass spec is just another detector. Most of us who work with LC-MS know that’s simply not the case – they can be maintenance intensive, unforgiving and generate complex information. When they’re not working it can be difficult to work out exactly where the problem lies. Here’s some advice to point you in the right direction
A question about column dead volume. If the hold-up volume (time) is the extra-column dead volume expressed in time or volume. What is the column dead volume and how can it be calculated?