Chiral Chromatography

At Pittcon 2024, LCGC International editor Patrick Lavery sat down with Martina Catani of the University of Ferrara to talk about the work her laboratory group is conducting.

Researchers from Hebei University in China have developed and characterized three novel peptide chiral stationary phases (CSPs) for enantiomeric separation, demonstrating their potential in high performance liquid chromatography (HPLC).

A recent review article discussed advances in liquid chromatography (LC) enantioseparations.

Since similar chiral recognition mechanisms were observed for benzoin (B) and methyl mandelate (MM), a three-site enantioselectivity model was proposed to interpret the data being sought by researchers.

What can chiral capillary GC do for you? The answer lies in what analysis one is attempting to accomplish. Here, we discuss the utility of chiral capillary GC and where the technique is most valuable, focusing on three application areas in particular.

Although closantel (CLO) is well-regarded as a curative antiparasitic drug, even low doses have been found to cause serious side effects in the human retina and central nervous system.

Researchers have synthesized a chiral metal-organic framework (MOF) called L-histidine-zeolitic imidazolate framework-67 (L-His-ZIF-67) for enantioseparation of drugs in capillary electrochromatography. The chiral MOF demonstrated excellent separation efficiency for five chiral drugs, offering promising possibilities for pharmaceutical analysis and quality control.

A talk by Alberto Cavazzini of the University of Ferrara at HPLC 2023 focused on various types of chiral stationary phases (CSP) in efforts to improve efficiency.

Mono-type, blending-type, coating-type, and bonding-type chromatographic packings based on chiral metal-organic frameworks (CMOFs) were analyzed regarding their effectiveness in enantioseparation.

The occurrence of convex-upward van Deemter curves is rare but not unusual in chiral liquid chromatography. In this work, this behaviour, experimentally observed for the more retained enantiomer of a chiral sulfoxide on a polysaccharide-based CSP, is explained.

Researchers at Xuzhou Medical University have developed a new type of chiral stationary phase (CSP) for HPLC that can be used under normal-phase, reversed-phase, and polar organic mobile phase conditions.

Researchers have produced new frameworks that may lead to significant advancements in chiral analysis.

In this study, various ways of achieving resolution by playing with selectivity are presented.

Having a mixed-mode column that can provide both achiral and chiral resolution in one-dimension (1D) would significantly improve impurity profile understanding and reduce testing workload.

Mirtazapine is an approved antidepressant, with other studies exploring its use to treat sleep apnea, substance abuse withdrawal symptoms, and as an antihistamine.

Improved analysis of pharmaceutical and natural medicine products requires advances in reversed-phase LC stationary phases. We examine two synthesized stationary phases with applicability in quality control and chiral separation for analysis of natural products.

The Dal Nogare award honors an awardee who is selected for contributions to the fundamental understanding of the chromatographic process. This year’s award winner is Professor Apryll Stalcup from Dublin City University.

The talks in this Friday afternoon symposium will cover topics related to column and stationary phase development and instrumentation, along with the analytical and production-scale applications of chiral chromatography.

Compared to the progress made in reversed phase separations in terms of speed and efficiency, chiral chromatographers have traditionally focused on improving the selectivity of enantiomeric separations by synthetic procedures. As a result, more than 50 different types of advanced chiral stationary phase chemistries are available today. Traditionally, speed and efficiency of chiral chromatography has not received attention until recently. With the availability of superficially porous particles and sub-2-µm particles, sub-minute enantiomeric separations have been achieved with the help of improved particle technology with narrow size distribution, and systematic studies on packing columns. This article covers advances made in the field of ultrafast chiral chromatography in the last decade. The development of instrumentation technology has also contributed immensely to making sub-second chiral separations a reality. Enantiomeric separations can now compete with the speed of sensors. Future directions and unanswe

LCGC, the leading resource for separation scientists, is proud to announce that Ronald E. Majors and Zachary S. Breitbach are the winners of the 11th annual LCGC Lifetime Achievement and Emerging Leader in Chromatography Awards, respectively. Majors and Breitbach will be honored in a symposium as part of the technical program at the Pittcon 2018 conference in Orlando, Florida, on February 26, 2018.

A chiral ionic liquid, namely 1-ethyl-3-methyl imidazole L-tartrate ([EMIM][L-Tar]), was applied as a new chiral ligand for the enantioseparation of tryptophan, tyrosine, and phenylalanine enantiomers by chiral ligand exchange CE. To validate the unique behavior of [EMIM][L-Tar], the performance of L-tartaric acid and 1-ethyl-3-methyl imidazole L-proline as chiral ligands was investigated to make a comparison with [EMIM][L-Tar]. Then the separation mechanism was further discussed. It was proven that [EMIM][L-Tar] was a good chiral ligand and would have good application prospects in separation science.

This article gives a brief overview of just some of the chiral environmental studies carried out recently that cover the differing enantiomeric activity of pesticides, their environmental transformation, and the degradation of pollutants in general. They highlight some of the recent advances in chiral stationary phases (CSPs) that have enabled higher efficiency and faster separations than previously seen in this area.

The majority of the samples in this study and other studies show that a majority of compounds can be separated by the use of the three most common alcohols: IPA, CH3OH, and ethanol. Solubility becomes a crucial factor in preparative chromatography. Combinations of solvents can be used to improve solubility when the chosen co-solvent has poor solubility of the compound in question.

In recent years, in the course of pharmaceuticals purified, as the need to chiral purification decreased since the improving of the asymmetric synthetic techniques, the desire to make medicine effective yet with a smaller number of doses and promised safety has raised

The need for chiral detection in the development of pharmaceuticals is increasing due to regulatory changes and the demand for single-isomer pharmaceutical drugs

A review of chiral separations, which remain a decided area of interest, particularly in the pharmaceutical and agrochemical fields

The establishment of a system suitability plan that focuses on the maintenance of the most important variable in any chiral separation screen - the column - is presented.