LCGC Interviews

Lapo Renai from the Van 't Hoff Institute for Molecular Sciences, The Netherlands discusses a novel prediction model for estimating chemical space coverage for non-targeted LC–ESI–HRMS workflows.

Sheher Mohsin and Jeremy Koemel walk through a real CCS Atlas example showing how homologous series confirm unknown PFAS identifications.

Jeremy Koemel discusses detecting low-intensity PFAS features when MS/MS signal is too weak, and how they work around it.

As metabolomics datasets balloon, Siuzdak explains using retention time and in-source fragmentation checks to separate real molecules from artifacts.

Sheher Mohsin explains how CCS measurements add a physical confirmation layer beyond mass and retention time in PFAS analysis.

Siuzdak separates true standard-based empirical spectra from artifactual signals, warning AI trained on flawed data risks perpetuating errors.

Gary Siuzdak explains how uMRM converts inconsistent untargeted LC–MS data into standardized MRM transitions.

James Grinias from Rowan University, New Jersey, USA, discusses sustainability and modern capillary LC systems.

Jim Grinias from Rowan University, NJ, USA highlights advances in miniaturized capillary LC–MS that simplify system setup and improve ease of use. He also discusses low-flow challenges and how integrated components can enhance performance and reproducibility.

Jonathan Maurer from the University of Geneva, Switzerland describes using HILIC for oligonucleotide analysis in practice and the role of HILIC for other biopharmaceutical applications.

Jonathan Maurer from the University of Geneva, Switzerland describes some of the challenges and misconceptions surrounding hydrophilic interaction chromatography (HILIC) and oligonucleotide analysis.

Wallman argues proteomics is moving from niche tool to high-throughput platform core, with the greatest untapped potential in covalent drug programs and targeted protein degradation.

Wallman explains that PeptDeepKontext is designed to generalize without lab-specific calibration, though highly unusual setups may still benefit from additional fine-tuning in the future.

Wallman unpacks how graph-level fragmentation in fragDETR captures internal fragments and neutral losses, with peak and intensity improvements concentrated in challenging non-standard peptides.

Nikoline Juul Nielsen from the University of Copenhagen, Denmark explores deprotomer formation for accurate CCS prediction and metabolite identification and the potential of implementing these workflows for routine use.

LCGC International spoke with Nikoline Juul Nielsen from the University of Copenhagen, Denmark on the benefits of RPLC×HILIC–cIMS for data-independent profiling of phenolic constituents in plant extracts. In this episode she explores practical aspects of cIMS and the potential for routine analysis.

Wallman explains how spectral library accuracy, retention time prediction, and instrument-specific variation make deep learning essential yet difficult in data-independent acquisition proteomics.

Wallman details PeptDeepKontext, a model built to predict peptide properties across diverse instruments and PTMs by embracing rather than eliminating inter-laboratory variability.

Exact mass, isotopic distribution, and MS/MS library matching are used together to differentiate PFAS hits from sulfur-rich food compounds that share a similar mass defect range.

Antonio Ferracane addresses regulator concerns about cleanup-free PAH methods, noting cleanup isn't mandatory if matrix validation proves reliable.

The approach remains non-targeted by prioritizing PFAS-like ions in the first DDA pass; the tunable mass tolerance window can be adjusted to capture structurally unusual PFAS subclasses.

Christine Fisher describes her method using mass defect filtering at the data acquisition stage to improve non-targeted PFAS detection in complex food matrices.

Antonio Ferracane explains how each sample prep step adds error and can strip target PAHs, so skipping cleanup can improve recovery, accuracy, and precision.

Antonio Ferracane discusses moving to minimal sample preparation for PAH analysis, and why matrix-matched calibration can replace cleanup steps.

Antonio Ferracane outlines a combined technique using cryogenic peak compression and pseudo-MRM to sharply boost sensitivity for trace PAHs.