Application Notes: LC/HPLC

Sep 22, 2017
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Environmental laboratories routinely evaluate VOCs in water via P&T GC-MS analysis. Because of the sensitivity of this method, it is critical that water used to prepare blanks, standards, and dilute samples be free of the VOCs to be analyzed. After purification by Milli-Q® water purification systems and a point-of-use VOC-Pak™ cartridge, product water is free of any compounds that could interfere with P&T GC-MS analysis of VOC levels.
Sep 21, 2017
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By Advion
High-profile murder cases have forced forensic labs to improve detection of EDTA in blood. Learn how LC and a single-quad compact mass spectrometer can quickly screen.
Sep 20, 2017
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Cannabis testing labs typically analyze for the 5 to 10 most common cannabinoids. This method targets 11 cannabinoids, including THCV, with an instrument cycle time of less than 10 minutes. The high efficiency chromatography produces sharp chromatographic peaks for the best overall sensitivity.
Sep 13, 2017
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Accurate mass determination of a bispecific T cell engager (BiTE®) consisting of two single-chain variable fragments (scFvs) recombinantly linked by a nonimmunogenic five-amino-acid chain was analyzed by SEC–MS using a TSKgel® UP-SW3000, 2 µm column under nondenaturing conditions. No signs of particle shedding or sample carryover, which may interfere with MS signal response, were noted in the analysis.
Sep 13, 2017
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This application note outlines the separation and analysis of a Fab-arm and PEGylated Fab-arm species isolated from a native monoclonal antibody using a TSKgel SuperSW3000 SEC column.
Sep 13, 2017
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This application note describes the use of a TSKgel UP-SW3000, 2 µm column for the analysis of proteins. Compared to SEC columns packed with larger particle sizes, the TSKgel UP-SW3000 column offers higher resolution and shorter analysis times.
Sep 13, 2017
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TSKgel STAT series columns provide high resolution separation of charge isoforms in a short analysis time and are ideally suited for the QC of biotherapeutics by UHPLC or HPLC. This application note presents the fast analysis of mAb charge heterogeneity by cation exchange chromatography using TSKgel STAT columns.
Sep 13, 2017
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This application describes the use of a 4.6 mm ID × 15 cm TSKgel® UP-SW3000, 2 µm SEC column for fast and accurate mAb aggregate analysis without compromising the quality of the aggregate determination or reproducibility. Results show that the run time of the 15 cm column was completed in 4 min without compromising the resolution of the chromatogram.
Sep 13, 2017
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HILIC chromatography with an amide-based stationary phase, used in conjunction with fluorescent-labeled oligosaccharides, is a well-established robust technique used by many laboratories to obtain high resolution separation of glycans. The application of a TSKgel Amide-80, 2 µm column for the analysis of glycans from monoclonal antibodies is demonstrated in this note, including a comparison to a 3 µm Amide-80 column.
Sep 13, 2017
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In this application note, an ADC was separated using a TSKgel® Butyl-NPR column, the least hydrophobic of the TSKgel HIC columns. Both unconjugated and drug conjugated Trastuzumab samples were successfully separated with baseline resolution. The baseline resolution enabled an easy integration and quantification of different drug pay loads in ADC characterization.
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