Application Notes: Supercritical Fluid Chromatography (SFC)

Sep 01, 2008
The Application Notebook
In the past decade, supercritical fluid chromatography (SFC) has experienced a striking resurgence and exponential growth in acceptance, particularly in pharmaceutical and chemical laboratories. In SFC, "supercritical" CO2, in combination with one or more polar organic solvents, most commonly alcohols, are used as mobile phase. The polarity of CO2 is similar to that of hexane, and thereby making SFC a normal phase chromatographic technique. SFC has readily lent itself as an attractive complement to reversed phase HPLC (RPLC). For instance, in separating polar compounds that have little retention, and/or selectivity, even with special polar group embedded columns, SFC holds a unique advantage over RPLC due to its normal phase separation mechanism.
Dec 02, 2006
The Application Notebook
As synthetic routes to create active pharmaceutical ingredients (APIs) increase in complexity, the analysis of the reaction mixtures becomes more difficult. Chromatographic techniques have been adopted by the pharmaceutical industry to solve analysis and purification problems. The lower viscosity of supercritical fluids, such as carbon dioxide, enables faster flow-rates than HPLC. Also, higher diffusivity yields greater efficiency (smaller plate heights) reducing column length required to resolve a sample. Shorter column lengths and an increase in flow-rate greatly reduces chromatographic time, thus supercritical fluid chromatography (SFC) is amenable to increasing the throughput of analysis or purification. SFC has, therefore, matured as a technique of choice for pharmaceutical analysts.
Jul 02, 2006
The Application Notebook
Increase efficiency and reduce analysis time by incorporating a new technology without learning more software.
lorem ipsum