Special Issues-04-01-2010

This discusses using generic chiral stationary phase screening to find the optimum column, smaller diameter columns for HPLC and SFC, clones of off-patent phases, and new concepts in CSP development.

Ron Majors reports on some of the improvements that have been made over the years focusing on efficiency, stability, and selectivity.

In this article, Chris Pohl summarizes some of the stationary phases that have been developed for modern ion-exchange and ion chromatography.

Improvements in HPLC column technology continue. Since the last Special Supplement on columns in 2008 (1), the continued need for high-throughput separation, the encountering of more complex samples, the need to analyze very polar compounds more easily, and the increased availability of very high pressure instruments has placed additional demands on the HPLC column. In terms of high throughput, no less than 24 companies have responded and have developed small particle columns of 2 µm and under. In addition, the number of superficially porous particle (SPP) columns has expanded, and users are finding that the high-efficiency-at-low-pressure advantage brings them added advantages. With more complex samples, longer columns packed with sub-2-µm particles require higher pressures with pumps now available to 1300 bar. It will be of interest to see if the lower pressure, high-efficiency SPP columns and monoliths, the latter of which have seen advances in the research of polymeric monoliths for applications to..

Liquid chromatography has long been used for the separation, analysis and purification of biomacromolecules.

Monolith pioneer Frantisek Svec brings readers up to date on the latest developments in monoliths for use in chromatography.

Rapid Method Development for Industrial LC Separations Using Automated Screening of Stationary Phases and Solvents.

The answer to the following reader question about degassing mobile phase solvents was supplied by LCGC's "LC Troubleshooting" columnist John Dolan.