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Deirdre Cabooter on Method Development in UHPLC: Performing ultrahigh-performance liquid chromatography (UHPLC) can significantly reduce analysis time without compromising on efficiency by using sub 2-?m particles that allow increased velocity while maintaining selectivity. In this new short interview, Deirdre Cabooter of KU Leuven in Belgium, discusses the advantages of using UHPLC in method development and tips to follow when transferring methods from UHPLC to HPLC instrumentation.


  • RESOURCE: Total Water Solutions Guide: A tool to guide you through the major steps in every pure water installation project, helping you to design and configure the most efficient total pure water system.

  • VIDEO: LC–MS System:Check out this video of the triple quad LCMS-8050, which features ultrafast polarity switching (5 msec), an ultra-high-speed scan rate of 30,000 u/sec, and up to 555 MRMs/second.

  • VIDEO: The Pros and Cons of GCxGC: In a new interview, Frank L. Dorman of Penn State University talks about the evolution of GCxGC and the key advantages and disadvantages of the technique, including why fear of its complexity gets in the way of its broader use.

  • RESOURCE: Method Transfer from HPLC to UHPLC:The Analysis of Fullerenes by Liquid Chromatography Mass Spectrometry.

  • LGCC TV: Mary Ellen McNally on Common Error with Internal Standard Methods.

  • TRAINING: CHROMacademy:Chromatography and Mass Spectrometry training covering subjects such as HPLC, GC, SPE, Mass Spec, and HILIC.

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Sample Prep Perspectives | Ronald E. Majors:

LCGC Columnist Ron Majors, established authority on new column technologies, keeps readers up-to-date with new sample preparation trends in all branches of chromatography and reviews developments in existing technology lines.

LATEST: The Role of Selectivity in Extractions: A Case Study

History of Chromatography | Industry Veterans:

With each installment of this column, a different industry veteran covers an aspect of the evolution and continued development of the science of chromatography, from its birth to its eventual growth into the high-powered industry we see today.

LATEST: Georges Guiochon: Separation Science Innovator

MS — The Practical Art| Kate Yu:
Kate Yu is the editor of 'MS-The Practical Art' bringing her expertise in the field of mass spectrometry and hyphenated techniques to the pages of LCGC. In this column she examines the mass spectrometric side of coupled liquid and gas-phase systems. Troubleshooting-style articles provide readers with invaluable advice for getting the most from their mass spectrometers.

LATEST: Mass Spectrometry for Natural Products Research: Challenges, Pitfalls, and Opportunities

LC Troubleshooting | John Dolan:

LC Troubleshooting sets about making HPLC methods easier to master. By covering the basics of liquid chromatography separations and instrumentation, John Dolan, Vice President of LC Resources and world renowned expert on HPLC, is able to highlight common problems and provide remedies for them.

LATEST: LC Method Scaling, Part I: Isocratic Separations

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GC in Forensic Laboratories
Tom Brettell recently conducted a survey of GC column use in forensic laboratories in the United States. Here is what he found, in terms of the types of columns they are using and the problems they face.
Classification of Columns for SFC Use
A column classification system for SFC to help with method development has been developed. This article demonstrates how the system can be used to select a column when developing a new SFC method.
The Storage and Use of Gases for Gas Chromatography
A discussion on the safe storage and use of calibration, carrier, and detector gas cylinders for small or large organizations.
New Chromatography Columns and Accessories at Pittcon 2014, Part I
New product introductions in the areas of HPLC columns for reversed-phase LC, HILIC, ion-exchange and ion chromatography, SEC, and specialty columns for specific types of analyses are covered.
Metabolomics in Food and Nutrition Laboratories
A look at the various strategies and tools related to metabolomics applied to food and nutrition


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