Biotherapeutics such as monoclonal antibody-derived molecules as well as their impurity profiles are becoming more complex. That challenges the routinely applied analysis of high- and low-molecular weight impurities by size exclusion chromatography and UV-absorbance. Possible limitations of SEC-UV can be overcome by adding advanced detection technologies such as multiangle light scattering (MALS) and mass spectrometry (MS). This podcast will explain the different detection methods and the results they deliver. Special emphasis will be placed on the higher requirements for size exclusion columns and methods preceding advanced detection methods.
Synthetic Peptides: Chromatographic Methods for Separation, Quantification, and Characterization
May 9th 2024Peptides are versatile molecules that have a wide range of applications in biotechnology, medicine, and research. They can be used as drugs, diagnostics, biomarkers, or tools to study cellular processes. However, peptides also pose unique challenges in terms of analysis and purification due to their complexity, diversity, and sensitivity.
Investigating PFAS in Plastic Food Storage Bags Using LC–MS/MS
May 8th 2024Yelena Sapozhnikova from the United States Department of Agriculture spoke to The Column about her innovative research investigating PFAS in plastic food storage bags using targeted and non-targeted liquid chromatography–tandem mass spectrometry.