How can analysts resolve impurities that differ by a single mass unit? Experts discuss deamidation, racemization, HRMS, and 2D-LC for GLP-1 peptides.
|Articles|June 9, 2015
Advancing Biopharma Analysis with Light-Scattering Detection
Author(s)Wyatt Technology Corporation
To characterize biopharmaceuticals, particularly monoclonal antibodies and antibody–drug conjugates (ADCs), you need a complete toolbox of powerful tools. You are probably familiar with LC–MS methods. But have you seen what light-scattering detection can do?
Advertisement
Advertisement
Related to this article
"LC Troubleshooting" explores why phosphorothioate oligonucleotides (ONs) diastereomers challenge liquid chromatography.
This new high-performance liquid chromatography (HPLC) method reliably measures cholesterol adsorption in artichoke fiber.
The panel walks through confirming peptide identity–from HRMS and LC–MS/MS sequencing to amino acid analysis and D/L-isomer verification.
Malte Hübschen of the University of Duisburg-Essen (Germany) discusses an automated gas chromatography-tandem mass spectrometry (GC-MS/MS) workflow that improves phthalate detection in tricky oils with LCGC International.
Kinjal Bhatt says belonging comes from team culture, not headcount, while Ina Varfaj's goal as her lab's only woman is making sure she isn't the last.
Liquid chromatography–ion mobility spectrometry–high-resolution mass spectrometry (LC–IMS–HRMS) shows elephant seal pups carry more PFAS than their mothers do.
Gas chromatography–mass spectrometry (GC–MS) and omics profiling distinguish duck and goose foie gras composition.
Advertisement
Advertisement