Application Notes: Size-Exclusion Chromatography (SEC)
AAV Aggregate Analysis Mobile Phase Robustness
Biozen dSEC-7 accurately resolves AAV serotypes 5, 8 and 9 monomer from aggregates in under 10 minutes with consistent results across different mobile phase conditions.
Therapeutic Proteins Analysis Using Size Exclusion Chromatography
This application note demonstrates the performance of BioCore SEC columns for determining aggregation and fragmentation associated with antibody-based biotherapeutics.
Assessing Protein Aggregation with SEC-MALS
Determine the molecular weight of low abundant species with confident with the highly sensitive DAWN MALS detector and improved peak separation with Waters BEH Column.
Analysis of mAbs, biosimilars and AAVs
Explore SEC-MALS-RI multi-attribute quantification of antibodies, biosimilars, and AAVs.
SEC-MALS and Fluorescence detection of AAV
Explore the optimization of AAV separation and the combined intrinsic fluorescence detection with SEC-MALS for low-concentration measurement.
AEX-MS analysis of an IgG4-based mAb
In this application note, the charge variant characterisation of an IgG4-based mAb was performed using a BioPro IEX QF anion exchange column coupled to an MS detector.
GPC/SEC-Viscometry for Structural Analysis
GPC/SEC-viscometry enables measurement of true molar masses for carrageenan and detects subtle differences between samples with similar molar mass distributions.
GPC/SEC-MALLS Analysis of Hyaluronic Acid (Aug 2023)
GPC/SEC-MALLS analysis of hyaluronic acid obtains absolute molar mass while conventional GPC/SEC with concentration detectors only provides apparent molar masses.
Light Scattering and Size Exclusion Chromatography
Discover how SEC can be used in biopolymer applications including bioconjugates, proteins, mAbs, mRNA1, viruses, extracellular vesicles, and liposomal nanoparticles.
Towards Greener GPC/SEC
This paper discusses sustainable solutions for size separation of macromolecules that align with the 12 principles of green chemistry.
GPC/SEC Applications - Polymers, Biopolymers, Proteins
Tips & Tricks designed to support GPC/SEC users in their daily work, providing comprehensive overviews on different aspects of this powerful technique.
Saccharide and Polysaccharide Analysis (Aug 2023)
Robust, reliable GPC/SEC analysis of saccharides and polysaccharides can occur with very high resolution using Agilent SUPREMA columns with 3 and 5 µm particles.
PEGylated Antibody Fragment Analysis with SEC-MALS
Pore Size Selection for SEC of Oligonucleotides (June 2023)
Columns of the YMC-Pack Diol SEC series (120–300 Å), which have distinct pore sizes, were tested for their ability to separate DNA of various lengths (10–300mer).
Sensitive Quantification of AAVs/AAV Impurities
Determine down to 8x10^9 AAV capsids and separate impurities of AAV samples by separation using a mixed-bed SEC column & highly sensitive MALS detection.
SEC-MALS Characterization of a Drug-delivery Polymer
Learn the importance of light scattering detection to determine the true molecular weight distribution of a poly(glutamic acid)-based copolymer for drug-delivery.
Compound Separation with Size-Exclusion Chromatography
This application note introduces application data obtained by using FlexFire SEC Columns that have adopted newly developed superior silica.
Absolute Molecular Weight Determination of Natural Polymers Using ShodexTM OHpakTM LB-806M and MALS Detection
Shodex™ has successfully developed the LB-800 columns which are suitable with MALS detection. The columns are well suited to provide signal at low concentration with low molecular weight standards.
New Shodex™ OHpak™ LB-800 HQ GFC Columns for Low Baseline Noise MALS Analysis
Shodex™ has SB-800 HQ series for the analysis of water-soluble molecules and for the determination of molecular weight distribution.
Shodex™ OHpak SB-806 HQ SEC Multi-Faceted Analysis of Exosome (EV) Combination of UV, Florescence, and Light Scattering Detectors
This technical article introduces an example of multi-faceted analysis and monitoring of an EV sample purification process using a SEC column, Shodex™ OHpak SB-806 HQ, with 3 different detectors.
Purification and SEC/MALS Analysis of Norovirus Virus-Like Particles Comparison Using the ShodexTM OHpak SB-805 HQ
When selecting a suitable analytical SEC column, not only the analytical range for the fractionation, but adsorption of the target compounds, which influences the quantification and yield, should also be considered.
Monitoring Structural Changes in Polysaccharides Using SEC-MALS
This application note explains how SEC coupled with MALS can be used for the determination of structural changes in polysaccharides.
SEC Columns for Large and Small Molecules
This application note introduces some new application data obtained by using FlexFire SEC that is adopted by newly developed silica gel.
Characterizing Lipid Nanoparticles (LNPs) for RNA Delivery
Field-flow fractionation coupled to multi-angle light scattering (FFF-MALS) is a powerful analytical tool that determines multiple CQAs of LNP-RNA nanoparticles.
Characterizing Branched Polymers by Light Scattering
SEC with multi-angle light scattering (SEC-MALS) or in conjunction with a viscometer (SEC-MALS-IV) effectively characterizes branching in polymers.
Understanding Antibody and Viral Glycoprotein Interactions (Sept 2022)
SEC-MALS and CG-MALS characterize viral glycoprotein (vGP) self-association and the absolute stoichiometry and affinity of vGP: antibody interactions.
Batch-to-Batch Comparison of Sub-2 µm Diol-Linked Size-Exclusion Columns (August 2022)
NIST mAb was analyzed using six different batches of 1.8 µm 200 Å dSEC-2 media packed with 150 mm length and 4.6 mm inner diameter columns.
White Paper: Size-Exclusion Chromatography (SEC) for the Determination of Protein Hydrodynamic Radius (August 2022)
Protein standards explored with known hydrodynamic radii, show how this can determine ideal size-exclusion conditions for optimal method performance using Biozen columns.
Improving Resolution for Size-Exclusion Chromatography Methods (August 2022)
Size-exclusion method improvements by running at a lower flow rate for the aggregate analysis of NIST mAb using a Biozen dSEC-2 column
Thermal Extraction of VOCs Using a Multi-ramp Method
This application note demonstrates a quick temperature ramp technique to improve the focusing of VOCs without a need for trapping.