Application Note: UHPLC Analysis of Six Common Nitrosamines
Six nitrosamines are separated using a YMC-Triart C18 UHPLC column. This is challenging due to the commonly observed small peaks thanks to low impurity concentrations.
UHPLC Analysis of Six Common Nitrosamines
Analyzing SARS-CoV-2 Drugs Remdesivir and GS-441524
A fast separation of remdesivir and its active metabolite GS-441524 for the treatment of COVID-19 is obtained in only 3 minutes using a YMC-Triart C18 UHPLC column.
Application Note: Native Online HIC–MS Analysis of mAbs
Charge variants of 28 mAbs were analyzed under MS-compatible conditions using YMC’s BioPro IEX SF column. Each mAb of different pI was separated from its variants
Charge Variant Analysis of 28 mAbs
Leuprorelin Analysis Using YMC-Triart Bio C18
In reference to the Japanese Pharmacopeia, YMC-Triart Bio C18 was used to analyze the peptide leuprorelin (GnRH analogue) and separate it from its degradation products.
Analysis of Bevacizumab by SEC–MALLS
In this application note, YMC’s dedicated SEC column for antibodies, YMC-SEC MAB, has been used to develop a method for bevacizumab using MALLS detection.
Application Note: Native Online SCX-MS Analysis of mAbs
LC–HRMS Analysis of the ASO Mipomersen
Mipomersen was analyzed by LC–HRMS using a biocompatible YMC-Triart C8 metal-free column ensuring symmetric peaks, high sensitivity, and no carryover effects.
SEC Separation of ADC Brentuximab Vedotin
The antibody-drug conjugate was separated from its aggregates and fragments in only one run using a YMC-SEC MAB column and varying mobile phases.
Application Note: Native Online HIC–MS of Cys-linked ADCs
In this application note, the DAR of the SigmaMAb ADC-mimic was determined using YMC’s BioPro HIC BF column and a nanospray ionization mass spectrometer.
LC–HRMS Analysis of Antisense Oligonucleotide Mipomersen
Mipomersen (Kynamro®) is used as a model for the analysis of antisense oligonucleotides (ASOs) using LC-HRMS. The YMC-Triart C8 metal-free column used is an excellent choice for oligonucleotides.
Baseline Separation of Chlorophenols
The baseline separation of various chlorophenols, that only differ in the amount and/or position of the chlorine substituents, is made possible using YMC-Triart C18 ExRS.
High Throughput DAR Determination of Brentuximab Vedotin
YMC’s latest HIC column is used to analyse the DAR of Brentuximab Vedotin allowing higher flow rates and shorter run times thanks to the pressure resistant particle.
The Right HIC Column For Each Separation
YMC’s HIC columns, BioPro HIC HT, and BioPro HIC BF address separation requirements of different compounds. The separation behaviour of two mAbs is demonstrated.
Native Online HIC–MS Analysis of mAbs
This application note shows how a mixture of seven in-house mAbs, as well as the molecular variants of two mAbs, were characterised via HIC-MS using YMC’s BioPro HIC BF column.
Native online HIC–MS of cys-linked ADCs
In this application note the DAR of the SigmaMAb ADC-mimic was determined using YMC’s BioPro HIC BF column and a nanospray ionisation mass spectrometer.
HIC Analysis of Oxidised Monoclonal Antibodies
Monoclonal antibodies and their oxidised species are separated using YMC’s more hydrophobic BioPro HIC BF column, dedicated for low hydrophobicity proteins and oxidised mAb variants.
High Resolution CEX Analysis of mAbs
Four therapeutic mAbs of different species, isotypes, and pI together with their variants were analysed using pH and salt gradient mode using YMC’s non-porous BioPro IEX SF.
Commercial MAbs by MicroLC-MS/MS
YMC-Triart Bio C4 is used in YMC capillary column format to analyse and identify intact commercial mAbs by micro LC–MS/MS at an elevated temperature of 75°C.
Native Online SCX–MS Analysis of mAbs
In this application note high-resolution SCX was coupled directly to an ultrasensitive online native MS using YMC’s BioPro IEX SF to analyse mAb charge variants.
UHPLC Analysis of mAbs
This note using YMC-Triart Bio C4 shows a robust separation at high temperatures for three commercially available mAbs: ofatumumab, palivizumab, and panitumumab.
mAb Method Using Isopropanol in HIC
This application note shows how to achieve higher recoveries for commercially available MAbs using 15 % isopropanol as modifier in HIC mode.
SEC Analysis of mAb Fragments
The analysis of intact MAb and their fragments using the UHPLC compatible YMC-Pack Diol-200 column and specifically designed YMC-SEC MAB column is described.
Reaction Monitoring of ADCs
The effective separation of intact antibodies from ADCs with their single DARs using hydrophilic interaction chromatography linked to UV/Vis is shown.
Generic Analyses of mAbs with Reversed-Phase (U)HPLC
This application note demonstrates how to achieve robust chromatographic results for commercially available MAbs with higher sensitivity and sharper peaks at 80°C.
AEX Separation of Oligonucleotides
In addition to existing RP based methods, a method is presented for the determination of oligonucleotides using YMC’s strong, non-porous anion exchanger BioPro IEX QF.
High Temperature Reversed-Phase Analyses of mAbs
This application note shows how to achieve robust chromatographic results for two commercially available MAbs using a modern temperature-stable widepore RP-phase.
Sensitive LC–MS/MS Analysis of Sphingophospholipids
The application shows that by implementing a metal-free column hardware any tailing caused by phosphate groups is avoided.