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Per- and Polyfluoroalkyl Substances (PFAS) or Interference? Using High-Resolution Mass Spectrometry as an Investigative Tool in Food AnalysisMatrix interferences can impact routine analysis with triple quadrupole methods for monitoring and quantifying PFAS in food.
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LC–MS/MS for the Quantification of Tau and Phosphorylated Tau in Alzheimer’s Disease Patients’ CSFQuantification of Tau proteoforms in cerebrospinal fluid (CSF) could be useful to diagnose neurodegenerative diseases. LC–MS/MS results can help explain the underlying molecular mechanisms of disease evolution.
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Analytical Advantages of Highly Stable Stationary Phases for Reversed-Phase LCDuring the past five years, many manufacturers of HPLC columns have focused on improving stationary phase stability and reproducibility. In this study, the authors use a variety of test solutes to compare the efficiency, selectivity, and hydrophobic retention mechanisms of five commercially available HPLC columns based silica, alumina, zirconia, and polystyrene cross-linked with divinylbenzene as the support.
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Benefits of UHPLCThis application note shows the performance of the KNAUER AZURA UHPLC system to speed up your method and increase throughput preserving resolution.
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Improving on AOAC 2001.02: GOS Determination in Foods Using HPAEC–PADAn improved method for determining total galactooligosaccharide (GOS) content in food and supplements using high performance anion exchange chromatography with pulsed amperometric detection is described.
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Aggregate and Fragment Analysis in Therapeutic Monoclonal Antibodies Using On-Line Size-Exclusion Chromatography with Native Mass SpectrometrySize-exclusion chromatography (SEC), with the use of ammonium acetate buffer, can be coupled on-line to electrospray ionization MS for the characterization of size variants of therapeutic monoclonal antibodies (mAbs). A quadrupole time-of-flight (QTOF) MS system was employed, and the MS method was optimized to achieve favorable sensitivity for high-mass detection, while maintaining the structural integrity of the aggregates (or high molecular weight species) and fragments (or low molecular weight species).
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Breaking the Rules: Two- and Three-Dimensional Chromatography with Four Dimensions of Mass SpectrometryTwo-dimensional liquid chromatography (2D-LC) allows much greater resolution of peaks than is possible in a classical single dimensional separation. For the next development in separations, we employed 2D-LC in two highly orthogonal dimensions of separation with four mass spectrometers for detection, with parallel detection in each dimension. We have further broken ground by using three dimensions of separation with four mass spectrometers, using two parallel second dimensions.
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Go With the Flow: Thinking About Carrier Gas Flow in GCSuccessful GC analysis requires careful control of carrier gas. Here, we explain how to measure and control flow rate, use constant pressure vs. constant flow, and more.
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Stability and Recovery Influences of Benzo[a]pyrene, Benzo[a]anthracene, Benzo[b]fluoranthene, and Chrysene during Sample Preparation of Plant MatricesQuantification of European Union (EU)-priority polycyclic aromatic hydrocarbons (PAHs) in plant matrices is a crucial task. Various methods for enrichment and preconcentration, such as the preloaded-pipette tip solid‑phase extraction (SPE) (1), are available. Nevertheless, analyte recovery as a result of homogenization, sample preparation, and extraction are rarely discussed in the field of phytopharmacy. This study deals with the recovery in dry plant extracts, which are typically used in phytopharmaceuticals and reflect the actual polycyclic aromatic hydrocarbon content in the commercially available end product (2). The aim of this study was to monitor benzo[a]pyrene, benzo[a]anthracene, chrysene, and benzo[b]fluoranthene loss of spiked samples as a result of commonly-used sample pretreatment, extraction, filtering, and evaporating techniques in 1:1 (v/v) cyclohexane–ethyl acetate primulae flos and sambuci flos dry extracts. Results showed that improper sample preparation can lead to false results. In the case of benzo[a]pyrene with a deviation of 155% from the theoretical true value.
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Comparison of PFAS Sample Preparation Between WAX + Dispersive GCB and WAX/GCB Cartridges from Water and Soil Extracts Per Draft EPA 1633How to speed up sample prep time and equivalent acccuracy and precision.
