The Column-06-20-2016

Multidimensional liquid chromatography strategies are the most widely used method for increasing the number of spatially resolved components and reducing stress on mass spectrometric detection. However, the stress placed on a secondary dimension in a comprehensive on-line methodology is very high. An increasingly attractive approach is the coupling of high performance liquid chromatography (HPLC) with ion mobility spectrometry hyphenated to mass spectrometry (IMS-MS). Tim Causon and Stephan Hann of the University of Natural Resources and Life Sciences in Vienna, Austria, spoke to The Column about their work evaluating this approach and exploring its possibilities for metabolomics.

The Chromatography Society is holding a four-day educational event in the Lake District from 14–17 October at the Wordsworth Hotel in Grasmere. Aimed at post-graduate students and novice chromatographers, the event will focus on the fundamentals of liquid chromatography and its practical application in industry and academia.

Gas chromatography–mass spectrometry (GC–MS) may be a viable diagnostic tool for inflammatory arthritis according to research from the University of Amsterdam.

Click the title above to open The Column June 20, 2016 Europe & Asia issue, Volume 12, Number 11, in an interactive PDF format.

Click the title above to open The Column June 20, 2016 North American issue, Volume 12, Number 11, in an interactive PDF format.

Leap Technologies Inc. and Antec have announced a joint venture in the field of sample preparation for HDX–MS.

While conventional calibration for gel permeation (GPC) or size-exclusion chromatography (SEC) is useful, there are inherent disadvantages in this type of analysis that introduce experimental error. This uncertainty may cast serious aspersions on the rigour and utility of the results. Multi-angle light scattering (MALS) detection is quite simple to add to an existing chromatography system and can help overcome the challenges faced with single detector chromatography and conventional calibration-based methods. An alternative separation technique called asymmetric flow field-flow fractionation (AF4) offers tunable, column-free fractionation.

J.J. Kirkland and J.J. DeStefano spoke to LCGC about the important contributions Dr. Klaus Unger has made to chromatography as Dr. Unger celebrates his 80th birthday.

Incognito’s search for the Holy Grail(s) of chromatography continues.

Monoclonal antibody (mAb) treatment is among the most effective method of diagnosis and treatment of a broad range of diseases, including autoimmune disorders, cardiovascular diseases, infectious diseases, cancer, and inflammation. There is an extensive development pipeline for protein and mAb therapeutics, emphasizing the need for innovative and efficient analytical tools that result in faster analysis times. This article explores the use of supermacroporous particles for the analysis of mAbs.