May 8th 2024
Yelena Sapozhnikova from the United States Department of Agriculture spoke to The Column about her innovative research investigating PFAS in plastic food storage bags using targeted and non-targeted liquid chromatography–tandem mass spectrometry.
Why is Open-Access Hyphenation Important for Chromatographers?
October 4th 2023At HPLC 2023, John Langley, a professor at the University of Southampton in Southampton, UK, presented a talk called "(R)evolution of Hyphenations, Through Open-Access RP-HPLC–MS, Open-Access UHPSFC–MS and 2D-LC–IMS-MS in Academia". He spoke with LCGC on the importance of open-access hyphenation, and how to implement it in your research.
Multitargeted Internal Calibration Using LC–MS Quantifies Chronic Kidney Disease-Linked Metabolites
September 30th 2023Researchers have developed a multitargeted internal calibration method for precise quantification of chronic kidney disease (CKD)-related endogenous metabolites using liquid chromatography–mass spectrometry (LC–MS).
Using LC–MS to Determine Phospholipid Concentration in Plant-Based Oils
September 25th 2023Christopher Hudalla of ProVerde Laboratories Inc. discussed how using liquid chromatography–mass spectrometry (LC–MS) could be used to quantify specific classes of phospholipid (PPL) constituents that can help streamline cannabis extraction.
Tackling PFAS Complexity with HRMS and Bioanalytical Techniques
September 1st 2023There are many benefits of using high-resolution mass spectrometry (HRMS) in analyzing PFAS accumulating in living organisms. This article shows how HRMS, when combined with exposure-relevant mixtures, can help elucidate more about PFAS toxicity and exposure.
A Multiphase Sampling and Analytical Approach for Investigating Airborne PFAS Transmission
September 1st 2023The investigation of airborne PFAS transmission is important in preserving the outdoor urban environment. The authors explain why GC–MS/MS and LC–MS/MS are the best techniques for analyzing airborne PFAS.
Known and Novel Proteins in Pseudomonas stutzeri Detected by Bottom-Up Proteomics
August 18th 2023The combination of the “bottom-up” approach with digest-free, direct-sequencing proteomics, along with the addition of a proteogenomics pipeline that identified missing data, yielded the determination of almost 3000 proteins.
Enhancing Peptide Mapping Sequence Coverage Through an Automated Dual Protease Digest
August 1st 2023Trypsin is one of the most commonly used proteases in peptide mapping protocols because of its high level of specificity. However, trypsin alone is not always sufficient for full sequence coverage. In this article, the authors detail how trypsin was combined with chymotrypsin to overcome this, and the benefits of an automated platform.
Polysorbate Analysis in Biopharmaceutical Applications—A Snapshot of the Analytical Toolbox
An overview of different approaches for the qualitative and quantitative analysis of polysorbates.