This article focuses on two of the more common techniques applied to the analysis of a series of benzodiazepines in a biological matrix.
Sample preparation is used routinely for bioanalysis due to the complexity of the matrix. Typically this contains a range of compounds from simple salts to large protein structures, with concentration ranges covering many orders of magnitude. Mass spectrometry is commonly used for bioanalysis, which with the discovery of ion suppression, now requires the removal of the matrix.
Several approaches can be taken to remove the matrix, including turbulent flow chromatography, solid-phase extraction (SPE), liquid–liquid extraction, protein precipitation and ultrafiltration. This article focuses on two of the more common techniques applied to the analysis of a series of benzodiazepines in a biological matrix. It studies methods to optimize SPE and also demonstrates that the use of a generic method is not always ideal due to the amount of matrix components left in the final eluant.
Dispersive Liquid-Liquid Microextraction: A Review and Roundup of Green Sample Prep Advancements
May 15th 2024The still relatively new technique has distinct advantages, but a few of those benefits make it incompatible with some of the currently accepted principles of green sample preparation.
Investigating PFAS in Plastic Food Storage Bags Using LC–MS/MS
May 8th 2024Yelena Sapozhnikova from the United States Department of Agriculture spoke to The Column about her innovative research investigating PFAS in plastic food storage bags using targeted and non-targeted liquid chromatography–tandem mass spectrometry.