The Column
The design of strategies to reduce the activity of residual silanols in conventional alkyl-bonded columns is still one of the main challenges in the current separation of basic compounds by reversed-phase liquid chromatography.
The design of strategies to reduce the activity of residual silanols in conventional alkyl-bonded columns is still one of the main challenges in the current separation of basic compounds by reversed-phase liquid chromatography. The addition of reagents, such as amines, surfactants and ionic liquids to the hydro-organic mobile phases is a useful option that provides enhanced results. The effectiveness of an additive to suppress the silanol activity can be measured based on the changes produced in retention or peak shape. The former option cannot be applied when the anion in the additive is adsorbed on the stationary phase, at least partially. Based on the fact that the main interest in blocking the residual silanols is the enhancement in peak shape, a simple, reliable, practical and illustrative way of characterizing the peak performance using the information provided by the plots of the left and right peak half-widths versus the retention time was developed. This approach is applied here to a variety of C18 columns and additives.
Monitoring 5-Fluorouracil Levels in Dried Blood Spots with UHPLC-MS/MS
May 3rd 2024Researchers have utilized ultra-high performance liquid chromatography-tandem mass spectrometry (UH PLC-MS/MS) to monitor 5-Fluorouracil, a medication used to treat various cancers, in dried blood spots. This process offers a simpler, more efficient method than those in place involving the monitoring of plasma.
Metabolomics Meets Machine Learning: The Future of Nature-Inspired Drug Discovery
March 29th 2024Researchers at Enveda Biosciences are transforming nature into new medicines with their artificial intelligence (AI)-enabled industrial-scale drug discovery platform incorporating tandem mass spectrometry (MS/MS).
Element Launches New Pharmaceutical Testing Service
March 29th 2024Element Materials Technology introduced its Rapid Response Pharmaceutical Testing Service, providing expedited testing and focusing on various requirements including active ingredients and final drug products, extractables and leachables, degradation production, and elemental impurities.