Application Notes: LC
Warfarin Analysis Using Quasar AQ UHPLC Column
Retention under reverse-phase conditions as a consequence of polar end capping on stationary phase provides sites for interaction with the polar entities within Warfarin.
Sulfa Drugs Analysis Using Quasar C18 HPLC Column
This application illustrates the efficient separation of three sulfa drugs using a Quasar C18 HPLC column.
Amoxicillin Analysis Using Quasar C18 HPLC Column
The Quasar C18 HPLC phase offers high efficiency separation of the semi-synthetic antibiotic, amoxicillin.
Modelling Peptide and Protein Retention Times
We present retention-time models for peptides and proteins that link their elution times to solvent fraction and temperature.
An Easier Way to Track Impurities
Learn how impurity-monitoring software can improve your understanding of your process route and help you track fate and purge.
Increasing Reproducibility of Acidic Peptides in RPLC
Adsorption of metal-sensitive analytes in LC-based assays can negatively impact data. Learn how to minimize analyte–surface interaction and improve reproducibility.
Practical Tools for LC Method Translation
We discuss the theory, principles, and key equations required for method translation for both isocratic and gradient LC methods using the Avantor® ACE® Method Translator tool.
FcR Affinity Chromatography in Upstream Development
Elution profiles based on mAb glycan structures produced by the TSKgel® FcR-IIIA-NPR™ affinity chromatography column correlate with results from HILIC glycan profiling.
Method Development of IEX mAb Separations Using YMC BioPro IEX Columns and Dial-a-Mix
Highlighting the use of the dial-a-mix method for screening different mobile-phase pH conditions during method development of IEX charge variant analysis.
HPLC Separation of Cannabidiol and Melatonin on YMC-Triart C18
This application note details the development of a HPLC method for analyzing CBD and Melatonin in an over-the-counter sleep aid.
Separation of 11 Cannabinoids on YMC-Triart C18
This application note highlights the ability of YMC-Triart C18 to separate 11 common cannabinoids encountered during potency testing.
Preparative Purification of Cannabidiol from Hemp Oil Extract
A loading study for purifying cannabidiol (CBD) from a commercially available “high-CBD” hemp oil extract
Investigation of Pore Size, Particle Size, and Column Geometry Effects on SEC Analysis of Monoclonal Antibodies
Demonstrating the use of YMC’s new 2µm Diol stationary phase for scaling down size exclusion analyses
Separation, chemical identification, and size characterization of nanoplastic particles using Raman microscopy and FFF-MALS
Here we present an innovative approach for the characterization of nanoplastic particles using centrifugal field-flow fractionation coupled with UV, multi angle light Scattering detection, and raman microscopy.
Characterization of Organochlorine Pesticides in Black Tea
The Torion® T-9 GC/MS system has characterized several high boiling point organochlorine pesticides in tea samples in less than 10 minutes.
Analyzing Azithromycin Using a Quasar™ C18 Column
This application note discusses using a Quasar™ C18 column to analyze azithromycin, an antibiotic used to treat various bacterial infections.
Analysis of Veterinary Drugs in Chicken by SPME-UHPLC/MS/MS
SPME-UHPLC/MS/MS offers an efficient alternative for the analysis of veterinary drug residues in animal tissue as compared to traditional sampling procedures.
HPLC Analysis of the Synthetic Antibiotic Ciprofloxacin
The Quasar biphenyl HPLC phase offers high efficiency separation and excellent peak shape of the synthetic antibiotic, ciprofloxacin.
Analyzing Cephalosporins Using a Quasar Biphenyl LC Column
This application note describes using a Quasar biphenyl LC column in analyzing several cephalosporins, a mixture of first and second generation β-lactam antibiotics.
Method Validation for Atrazine Analysis
A highly sensitive, validated method for atrazine determination in different water types using the QSight 420 LC–MS/MS employed a simple filtration and direct injection.
Analysis of Challenging Polar Contaminants in Water
Using the LX50 UHPLC and QSight 420 triple quad MS, three analytical methods were developed to provide sensitive, reproducible results without the need for pre-concentration steps.
Analysis of PPCPs in Drinking Water
A methodology for a sensitive and repeatable analysis of PPCPs in drinking water utilizing the QSight SP50 Online SPE system coupled to a QSight 220 MS/MS.
Analysis of IgeS Digested Monoclonal Antibody Fragments
IdeS, a cysteine protease, is used for partial digestion of mAbs and generates a (Fab’)2 fragment and two Fc fragments (Fc/2) for better characterization of the Fc fragment.
Method Development of C18 Columns and High pH
Selectivity of two high-pH stable Agilent InfinityLab Poroshell 120 C18 columns is compared with pesticides using a pH 10 formate buffer and acetonitrile gradient.
Tandem Digestion of Monoclonal Antibodies Using Novel Cysteine Proteases
This application combines cysteine proteases IgdE and IdeS, to observe conserved and hypervariable regions of the antibody, as well as identification of other variants.
Analysis of IgdE Digested Monoclonal Antibody Fragments
IgdE allows for better characterization of the antibody and yields an intact Fc and an intact Fab fragment that can be further isolated and characterized.
Comparison of UHPLC Particles for Intact Mass Subunit Analysis of Monoclonal Antibodies
Comparing core-shell superficially porous to fully porous particles to determine efficiency required for detection, separation of low-level impurities for subunit analysis.
A Simple Quantitative Method for Monoclonal Antibody Coformulations
A reversed-phase method using a superficially porous, wide pore particle morphology for the quantitation of coformulated mAbs leads to high efficiency separations.
Improving Selectivity for Intact Mass Spectrometry of Biotherapeutic mAbs
For LC–MS analysis of NIST mAb, multiple C4 LC columns are compared to determine which provides necessary separation of impurity and avoids spectral overlap in the MS detector.
Improved Peak Shapes for Basic Analytes
The performance of basic pharmaceuticals is compared to the charged surface Agilent InfinityLab Poroshell 120 CS-C18 and a C18 with formic acid mobile phase.