May 8th 2024
Yelena Sapozhnikova from the United States Department of Agriculture spoke to The Column about her innovative research investigating PFAS in plastic food storage bags using targeted and non-targeted liquid chromatography–tandem mass spectrometry.
Developments in Preparative-Scale Chromatography Columns and Accessories
December 1st 2004This column focuses on some of the latest developments in preparative-scale columns, bulk packing materials and column hardware designs. Silica, polymeric and other packings are discussed together with the newest monolithic columns.
Facilitated Column Ranking and Selection in Reversed-Phase Liquid Chromatographic Analysis
November 1st 2004In this article, the authors present a column ranking system to facilitate column selection in reversed-phase liquid chromatographic analysis. The system ranks columns based on their similarity to a reference column of choice and its applicability to a real pharmaceutical analysis is demonstrated.
Elevated Temperature HPLC: Principles and Applications to Small Molecules and Biomolecules
October 1st 2004In this article the authors review the use of elevated temperatures in HPLC, and provide examples covering separations of both small molecules and biomolecules. Generic issues of temperature dependence of retention and plate height are discussed, and comparisons are made between temperature gradient and solvent gradient elution. They describe how the use of elevated temperatures allow good chromatographic efficiency to be obtained at flow-rates higher than those optimal at ambient temperature, thus increasing the speed of separation.
A Combination of Liquid Chromatography and Mass Spectrometry for Peptidomic Research
October 1st 2004The guest authors describe the use of LC–MS for the analysis of peptides. They deal with peptide extraction, peptidomic analysis in organisms with and without genomic databases, identification of peptides with conserved N- and C- terminal sequences, and in situ MALDI-TOF peptide profiling.
Shear-Driven Flow LC: Dispensing with Pumps and Voltage Supplied for Increased Speed and Resolution
May 1st 2004The authors discuss the application of shear-driven chromatography, a technique that depends on viscous drag to propel mobile phase in high-resolution/high-speed liquid chromatography. This method, it is claimed, overcomes the pressure- and voltage-drop limitations of both HPLC and CEC. Early development of the technique is reviewed and updated with the current status of the group's work. Also covered are the challenges faced and opportunities available to interface such systems to MS and UV/vis absorption detectors.
Parallel Chromatography — Double Your Money
May 1st 2004In this month's instalment, the authors cover the topics of column-switching techniques and parallel chromatography for LC?MS?MS methods, and describe the time benefits that can be achieved using such systems. An example set-up is presented and the authors outline the various steps required to perform such analyses routinely.