May 8th 2024
Yelena Sapozhnikova from the United States Department of Agriculture spoke to The Column about her innovative research investigating PFAS in plastic food storage bags using targeted and non-targeted liquid chromatography–tandem mass spectrometry.
New Chromatography Columns and Accessories at the 2004 Pittcon Conference, Part I
April 1st 2004In the first of a two-part series, Ron Majors examines new column introductions at Pittcon with special attention to high performance liquid chromatography columns and packings for reversed-, normal- and bonded-phase; ion exchange; ion and size-exclusion chromatography. He also considers speciality HPLC columns.
Success with Evaporative Light-Scattering Detection, Part II: Tips and Techniques
April 1st 2004Following on from a previous instalment, this month's column assesses evaporative light-scattering detection as a complementary detection mode and builds upon the previous discussion in terms of detector applications.
Analysis of Combinatorial Natural Products by HPLC and CE
February 1st 2004In this article the authors report on a combinatorial natural product discovery methodology that uses a viral vector system to transfer secondary metabolite-related enzymes from C. roseus to tobacco cell cultures. Using high-resolution separation techniques, including HPLC, CE and MS, they describe the analysis of secondary metabolite patterns.
Simulated Moving Bed Chromatography for Preparative Solutions
July 1st 2000This month's "Column Watch" describes moving bed chromatography and how the concept is realized in the form of simulated moving bed chromatography. Also included is an inside look at instrumentation, method development, and applications for industrial purifications.
Turbulent-Flow LC for LC–MS and LC–MS–MS Bioanalysis
This month's "Directions in Discovery" gives a brief overview of turbulent- flow liquid chromatography, a technique for fast separations. Two examples demonstrate the use of the technology for high-throughput bioanalytical separations in drug discovery.
Coupling Chiral Stationary Phases as a Fast Screening Approach for HPLC Method Development
June 1st 2000Given the increasing number of chiral samples and the time constraints under which chromatographers work, choosing an appropriate chiral stationary phase for the resolution of enantiomers can be challenging. In this article, the authors describe a screening approach for chiral HPLC method development.
Starting Out Right, Part VI – The Scouting Gradient Alternative
May 1st 2000A scouting gradient is a separation run under a standardized set of conditions and can be used to determine the complexity of a sample and estimate the difficulty of the separation. John Dolan tells why he believes using a scouting gradient is the best way to begin method development.