Column: LC Troubleshooting

Oct 31, 2017
LCGC North America
In his final column before retirement, John Dolan reflects on the changes he has seen during his 34 years as the author of “LC Troubleshooting,” and shares his short list of best practices that still hold true today.
Sep 01, 2017
LCGC North America
When developing or modifying an LC separation, a common strategy is to change selectivity by choosing a new column. Here’s guidance for making that selection.
Aug 01, 2017
LCGC North America
You have to be careful when adjusting gradient conditions.
Jul 01, 2017
LCGC North America
We consider some of the details associated with preparing a column for storage, with an eye toward choices that will pay dividends in future use.
Jun 01, 2017
LCGC North America
How much of a change can you make to a USP method without revalidating the method?
May 01, 2017
LCGC North America
To change selectivity in LC, which variable should you choose?
Apr 01, 2017
LCGC North America
Learn how to find the “sweet spot” in terms of retention for a liquid chromatography separation and how to estimate how much a change in mobile-phase percent organic will affect retention.
Mar 01, 2017
LCGC North America
Here’s how to estimate the effects of changes in column length and particle diameter without conducting experiments in the laboratory.
Feb 01, 2017
LCGC North America
Proper filtering is important to protect LC system components and deliver high quality analytical results. In some cases, however, it makes sense to deviate from best practices.
Jan 01, 2017
LCGC North America
Mobile-phase pH is a powerful tool, but must be controlled properly.
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