Application Notes: Sample Prep
Extraction of Novel Synthetic 2-Benzylbenzimidazole Opioid Compounds "Nitazenes" (Dec 2022)
This application note introduces a simple targeted extraction method for the analysis of nine nitazene compounds from urine and blood using the Clean Screen® DAU column.
Microwave Energy in Rapid Hydrolysis of Soy Flour
The nutrition profile of food is critical for assessing its quality. In this poster the amino acids in soy flour were hydrolyzed using a Discover Prep microwave system.
Methods for Analyzing Alternative Proteins
Plant-based proteins are becoming a mainstay to grocery stores, restaurants, and retailers. Amino acids in vegan cream cheese are herein hydrolyzed and analyzed.
Efficient Amino Acid Hydrolysis of Pet Foods
In this app note, we perform amino acid hydrolysis of proteins to identify and quantify each amino acid utilizing a safe and rapid amino acid hydrolysis protocol.
Amino Acid Hydrolysis Brochure (Dec 2022)
Protein analysis plays a large role in the production of foods, pet foods, and even pharmaceutical medicines. But first, the amino acids in the protein must be broken down.
Hydrolysis of Liraglutide Using Microwaves
Peptide therapeutics are held to high regulatory standards. Discover Prep was used to hydrolyze the amino acids of a peptide in only 15 minutes, instead of 24 hours.
Rapid Preparation of Infant Formula for AA Analysis
Classical protocol of amino acid hydrolysis needs up to 24 h of reaction time. Using the Discover Prep, the same level of acid and base hydrolysis was achieved in minutes.
Analysis of Free Steroids and Sulfate Conjugates (Nov 2022)
This app note outlines a highly efficient method for the simultaneous analysis of free and sulfated steroids from urine, plasma, and blood utilizing SPE and LC-MS/MS.
Automated SPE of PFCs from Tap Water
This application note describes the automation of the isolation of 10 perfluorinated compounds (PFCs) from tap water prior to their analysis by LC–MS.
Analysis of Cannabinoids in Blood
This application note outlines an extraction method for four natural cannabinoids and two major metabolites of Δ9-THC in blood using Clean Screen® THC SPE column.
Analysis of Free Steroids and Sulfate Conjugates
A robust analysis method for steroids was developed that can readily be implemented by clinical, forensic, or anti-doping laboratories.
Analysis of Cannabinoids in Urine
This application note features a unique, simple, and robust solid-phase extraction method for cannabinoids in urine followed by analysis with LC–MS/MS.
Extraction of Novel Synthetic 2-Benzylbenzimidazole Opioid Compounds "Nitazenes"
On-Column Digest Followed by SPE Desalting
Tecan’s proprietary NBE™ format enables digestion or incubation AND elution in a single SPE column. Read this app note to learn more and streamline your workflows!
An Optimized Protocol for the Clean-up of Oligonucleotides
The Clarity OTX 2 mg/well format is used for the isolation, characterization, and quantitation of current antisense oligonucleotide therapeutics from small biological fluids.
Comparing Supported Liquid Extraction and Liquid–Liquid Extraction: for extracting Acidic, Basic, and Neutral Analytes from Plasma
Advantages of using a supported liquid extraction (SLE) over liquid–liquid extraction (LLE) method for extracting a range of analytes from pig plasma.
Efficient Peptide Desalting Using Tecan’s Proprietary NBE™ Columns
Tecan’s proprietary NBE format SPE columns enable desalting in the column before peptide extraction. Read this app note to see how Tecan can accelerate your workflows!
Analyzing Microplastics in Salt Samples
Application note describing how thermal desorption–GC–MS is used to address multiple challenges in the detection of microplastics in different salt samples
Automated Extraction of Benzodiazepines from Urine
Benzodiazepines are depressants and used to relieve anxiety, insomnia, and more. This app note discusses a urine extraction to confirm the presence of benzodiazepines.
SPE of Opioids from Blood and Urine
A simple and robust method for the analysis of Opioids in biological matrices using UCT's Clean Screen® DAU SPE column and Selectra® DA UHPLC column.
Characterization of Adeno-Associated Viral Vectors
Quality attributes of Adeno-Associated Virus (AAV) vectors are achieved with multi-angle light scattering coupled to separation methods and high-throughput dynamic light scattering.
Evaluation of Mixed-Mode Ion Exchange SPE for the Extraction of Opioids from Human Urine by UHPLC-MS/MS
This application notes introduces an SPE LC–MS/MS methodology using the Microlute™ CP SCX SPE 96-well microplate for the determination of 12 opioids from human urine.
Application Note: Hydrocarbon Fractionation Using Enviro-Clean Fusion®Ag+
This application note outlines the fractionation of hydrocarbons into aliphatic and aromatic fractions using UCT’s new silver-phase EPH fractionation cartridges.
Extractions of THC-metabolites from Urine
This application note demonstrates a sample preparation procedure for using the Empore C18 SPE cartridge for the extraction of the THC metabolite, THC-COOH, from urine samples.
Selection of UHPLC Columns for Nucleic Acid Oligomer Analysis
Synthesized 40-mer miRNA has been analyzed using both a conventional column and new types of UHPLC columns.
Scaling for Efficiency and Throughput: LC Method Optimization with Notes on Selectivity and Preparative LC
MHC-Associated Peptide Purification for Immunopeptidomics
Automated MHC-I is associated peptide enrichment using the Agilent AssayMAP Bravo platform with large-capacity Protein A cartridges for immunopeptidomics analysis.
Quantification and Characterization of Biotherapeutics Proteins
The AssayMAP Bravo was used to purify, digest, and clean up antibodies from 96 cell culture supernatants with high reproducibility and minimal labor prior to LC–MS.
Intact Antibody and Subunit Mass Determination
Intact and subunit antibody mass determination was streamlined using automated purification and enzymatic cleavage and deglycosylation of immobilized antibodies.
Assessing Multiple CQAs for Monoclonal Antibodies v2
Automated sample prep and LC–MS peptide mapping approach to characterize and quantify multiple monoclonal antibody (mAb) critical quality attributes (CQAs)