Liquid Chromatography (LC/HPLC)

Dec 01, 2017
LCGC North America
To address the challenges of analyzing new illicit drugs, emerging techniques such as UHPSFC with MS and UV detection, and GC with VUV detection, may be needed, particularly for distinguishing positional isomers and diastereomers.
Nov 01, 2017
LCGC Europe
The last decade has witnessed how liquid chromatography columns and instruments changed from long bulky columns with relatively large fully porous particles operated at modest pressures (100–200 bar), to short compact columns with small superficially porous particles operated at ultrahigh pressures (1200–1500 bar). This (r)evolution has resulted in a tremendous increase in achievable separation performance or decrease in analysis time, but requires a good knowledge of optimal chromatographic conditions for each separation problem and, concomitant, the right instrument configuration.
Oct 01, 2017
LCGC North America
Dwight Stoll, who will take the reins of “LC Troubleshooting” next month, spoke with John Dolan to get some insight on the current state of chromatography training, future troubleshooting problems, John’s toughest troubleshooting challenge, and the most common chromatographic mistakes.
Oct 01, 2017
LCGC North America
By LCGC Editors
In food analysis, HPLC and triple-quadrupole mass spectrometry are established techniques. Jon Wong of the Center for Food Safety and Applied Nutrition at the U.S. Food and Drug Administration discusses why his agency and others have been developing food analysis methods using UHPLC and HRMS.
Oct 01, 2017
LCGC North America
In his final column before retirement, John Dolan reflects on the changes he has seen during his 34 years as the author of “LC Troubleshooting,” and shares his short list of best practices that still hold true today.
Sep 01, 2017
LCGC North America
This is an exciting time for 2D-LC, as the expanding community of users works to develop creative solutions involving 2D-LC to solve analytical challenges that are very difficult or impossible to solve by conventional means.
Sep 01, 2017
LCGC North America
When developing or modifying an LC separation, a common strategy is to change selectivity by choosing a new column. Here’s guidance for making that selection.
Sep 01, 2017
LCGC North America
Topics that stood out at HPLC 2017 include advances in large-molecule separations, multidimensional chromatography, HILIC, and chiral separations.
Sep 01, 2017
LCGC Europe
Monitoring lipid oxidation during the shelf life of lipid-containing food emulsions, such as mayonnaise, is challenging. It is, however, essential for the development of improved, consumer-preferred products. Determining the nonvolatile lipid oxidation products (NONVOLLOPS), the precursor compounds for rancidity, is required to determine the effectiveness of product stabilization technologies. A method based on normal-phase liquid chromatography with atmospheric pressure photo ionization-mass spectrometry (LC–APPI-MS) was developed for this purpose. The inclusion of a size-exclusion chromatography (SEC) step was needed to remove interfering diacylglycerides and free fatty acids from the samples. The combined SEC and normal-phase LC–APPI-MS method allowed the identification of a wide range of oxidized species including hydroperoxides, oxo-2½ glycerides, epoxides, and other oxidized species. The method was found to be more suitable for the analysis of large sample sets. The relative levels of NONVOLLOPS from bo
Aug 01, 2017
LCGC North America
UHPLC’s benefits include fast analysis, high-resolution separations, reduced solvent and sample usage, enhanced sensitivity and precision, and more.
lorem ipsum