Liquid Chromatography (LC/HPLC)

A liquid chromatography–tandem mass spectrometry (LC–MS/MS) method for the determination of 39 emerging contaminants in water at ultra trace concentrations has been developed and validated.

In this installment of “LC Troubleshooting,” we take a close look at how the commonly used “flow-through needle” sampler works, discuss how and where leaks can develop, and solutions to the problems when they do occur.

Collaboration between academia and industry can reap rewards that cannot be achieved separately.

A quality attribute of Suzuki-Miyura coupling reactions is to measure residual levels of phosphine ligands in the reaction product. Residual phosphine ligands can be present in non-oxidized and oxidized forms. The non-oxidized and oxidized forms have different UV chromophores and can have differing solubility. These differences can preclude accurate and precise quantification. The extent of oxidation is difficult to prevent and control for calibration standards and test materials. This paper describes a method for derivatizing residual phosphines to the oxidized form. The oxidized form is stable and provides a basis for accurate quantification of residual phosphines. This overcomes the challenges for quantifying the non-oxidized and oxidized forms.

Combining silicon micro-nanofabrication technology to create perfectly ordered separation beds on a silicon chip, such as micro-pillar array columns (µPACs), is a powerful innovation in liquid chromatography. This article discusses the numerous possibilities of micro-nanofabrication in the future of analytical chemistry in life science, covering sample preparation, separation science, and detection technologies.

The Chromatographic Society has announced the winners of the Martin Medal and the Silver Jubilee Medal for 2026. Professor Guowang Xu of the Dalian Institute of Chemical Physics in China, has been awarded the Martin Medal, and the Silver Jubilee Medal has been awarded to Professor Bo Zhang of Xiamen University in China.

In its 2025 industry survey, LCGC International looked beyond the numbers to uncover the challenges, priorities, and outlook shaping the chromatography community, and what lies ahead in 2026.

In this second installment of a multi-part series, we describe the principles of ion-pair reversed-phase high-performance liquid chromatography, discuss strategies for method development, and provide practical tips for robust liquid chromatography analyses of oligonucleotide mixtures.

The second part of the end-of-the-year review of LCGC International’s editorial coverage of HPLC 2025, which took place in Bruges June 19–23, 2025.

The first part of an end-of-the-year review of LCGC International’s editorial coverage of HPLC 2025, which took place in Bruges from 19–23 June 2025 .

The article discusses advancements in two-dimensional liquid and gas chromatography (LC×LC and GC×GC) to address the challenges of analyzing complex samples in non-target analysis.

Jessica Lin and Zhenqi (Pete) Shi from Genentech describe a novel machine learning approach to predicting retention times for small molecule pharmaceutical compounds across reversed-phase high performance liquid chromatography (HPLC) columns.

This article explores the diverse career paths where analytical sciences PhDs create impact in fundamentally different ways than traditional research roles, and provides practical guidance on how to pursue them.

What steps should be taken when reactivating your gel permeation chromatography/size-exclusion chromatography (GPC/SEC) instrument after an extended shutdown period?

A fast, selective, and quantitative liquid chromatography (LC) method was developed for lipid nanoparticles (LNPs) analysis, using evaporative light scattering for sensitive detection of the analytes of interest.

Discover the 2026 HTC Innovation Award, recognizing groundbreaking contributions in separation science and hyphenated techniques by emerging scientists.

This LCGC blog highlights the ACS SCSC’s initiatives, events, and awards that connect and support the global separation science community across academia, industry, and government.

In this LCGC International interview, David S. Hage discusses the latest advances in affinity chromatography and next-generation separation techniques for analyzing complex biological and environmental samples.

The 54th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2025), was held from June 15–19, 2025, in Bruges, Belgium. This “Column Watch” installment presents many of the highlighted topics and trends observed at this symposium.

Explore the complexities of GLP-1 impurity detection using HPLC/UHPLC and innovative strategies to enhance analysis and regulatory compliance.

Discover innovative LC–HRMS methods for analyzing RNA-based therapeutics, enhancing biopharma development and regulatory compliance in drug characterization.

Master LC troubleshooting techniques to optimize your analysis and resolve issues with confidence.

A reversed-phase liquid chromatography–charged aerosol detector (RPLC–CAD) method was developed and validated per ICH Q2(R1) guidelines for simethicone quantification.

How does liquid chromatography (LC) assist in forensic analysis?

The development of a high-throughput LC–MS/MS method for quantifying 135 pesticides with improved accuracy and robustness is discussed.