Liquid Chromatography (LC/HPLC)

Apr 16, 2018
The Column
The Column spoke to Arcadius V. Krivoshein, Assistant Professor of Chemistry at the University of Houston–Clear Lake, USA, about his work developing EPP, an experimental anticonvulsant that can help to stop convulsions during epileptic seizures, and the role of chiral HPLC in this research.
Apr 16, 2018
The Column
By LCGC Editors
A snapshot of key trends and developments in the chromatography sector according to selected panellists from companies who exhibited at Analytica 2018.
Apr 01, 2018
LCGC Europe
Our annual review of new liquid chromatography columns and accessories, introduced at Pittcon and other events.
Apr 01, 2018
LCGC North America
An overview and assessment of the latest HPLC and mass spectrometry instruments, modules, chromatography data systems, and related software.
Mar 01, 2018
LCGC Europe
Chromatographic principles and best practices for obtaining highly precise retention time, peak width, and resolution predictions for the optimization of reversed-phase liquid chromatography (LC) separations using retention modelling software will be discussed. The importance of fully characterizing the LC instrumentation, how to generate accurate input data, the selection of appropriate models, and peak tracking will be addressed along with a suggested workflow. Adhesion to a few basic rules and simple precautions and the use of modern retention modelling software programmes can assist the rapid development of highly accurate retention models to enable the development of robust and optimized reversed-phase LC separations using either ultrahigh-pressure liquid chromatography (UHPLC) or high performance liquid chromatography (HPLC) conditions. Examples of retention modelling for small and large molecules will be highlighted.
Feb 01, 2018
LCGC Europe
The drug discovery process can be accelerated by chromatographic profiling of analogs by measuring their nonspecific binding to proteins and lipids and then by modelling in vivo distribution. A balanced potency and chromatographically determined membrane and protein binding ensure the selection of compounds with the highest probability to show the desired in vivo distribution behaviour for efficacy and reduced toxicity. The first part of the article will discuss the high performance liquid chromatography (HPLC)-based measurements of lipophilicity and biomimetic properties, while the second part will discuss the models derived from the measured data of known drug molecules and drug discovery compounds.
Jan 01, 2018
LCGC Europe
Characterization of protein modifications is an essential aspect of biopharmaceutical development. Traditionally, the characterization process of chromatographic peaks involves manual, larger-scale fractionation to obtain a sufficient amount of material for further analytical studies. This article presents a fully automated process for online peak fractionation and reduction of therapeutic antibodies with subsequent quadrupole time-of-flight mass spectrometry (QTOF-MS) characterization. This innovative technique significantly accelerates MS peak characterization compared to traditional approaches and avoids the risk of unintended modifications of the variants as a result of the isolation process, for example, deamidation during storage of isoforms. This approach considerably reduces the required sample amount and can be used for the characterization of product-related impurities during early stage development.
Dec 01, 2017
LCGC North America
To address the challenges of analyzing new illicit drugs, emerging techniques such as UHPSFC with MS and UV detection, and GC with VUV detection, may be needed, particularly for distinguishing positional isomers and diastereomers.
Nov 01, 2017
LCGC Europe
The last decade has witnessed how liquid chromatography columns and instruments changed from long bulky columns with relatively large fully porous particles operated at modest pressures (100–200 bar), to short compact columns with small superficially porous particles operated at ultrahigh pressures (1200–1500 bar). This (r)evolution has resulted in a tremendous increase in achievable separation performance or decrease in analysis time, but requires a good knowledge of optimal chromatographic conditions for each separation problem and, concomitant, the right instrument configuration.
Oct 01, 2017
LCGC North America
Dwight Stoll, who will take the reins of “LC Troubleshooting” next month, spoke with John Dolan to get some insight on the current state of chromatography training, future troubleshooting problems, John’s toughest troubleshooting challenge, and the most common chromatographic mistakes.
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