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Nontargeted Screening for the Verification of Allergenic Ingredients and Perfume Authenticity by GC-ecTOF-MSIn this study, the experimental nontargeted screening approach and corresponding data analysis workflows—simultaneously using molecular ion information and structural information—are presented for the molecular identification and authenticity verification process from a brand perfume using GC–ecTOF-MS.
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The 26th Norwegian Symposium on ChromatographyThe 26th Norwegian Symposium on Chromatography was held 21–23 January 2024. The symposium has strong traditions in the Norwegian separation science community, serving as a forum for excellent scientific talks, networking, and social events.
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Simple Tips to Increase Sensitivity in U/HPLC AnalysisA main goal of any U/HPLC analysis is to achieve the optimum sensitivity with the given instrumentation. Here are a few simple measures that can be easily implemented.
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Coupling Static Headspace Sampling to High-Speed Gas ChromatographyRoutine analysis of volatiles from fruit juices can be sampled and concentrated using a static headspace method and rapidly analyzed by high-speed GC.
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SEP21 PreviewThe 14th Congress of the French-speaking Association for Separative Sciences (SEP21) will take place at the Porte de Versailles in Paris, France, from 5–7 October 2021. This preview explores what visitors can look forward to from the conference.
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Rolduc 2024Rolduc 2024 takes place from 28–30 April 2024 in Rolduc Abbey, Kerkrade, The Netherlands.
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Rapid Rinse and Shoot: A Screening Workflow for Pesticides in Fruit by GC–MS in Under Six Minutes Using Library Searching of Deconvoluted SpectraTrace-level pesticides and environmental pollutants in the food supply continue to be a worldwide concern. Here we present a fast-screening workflow for residual pesticides present on the surface of fruits using a GC–MS system with direct heating technology and MS spectral deconvolution.
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Effectiveness of High-Resolution Ion Mobility for Complex AnalysesHRIM has emerged as a robust separation strategy for complex chemical analyses due to its ability to improve peak capacity and aid in the separation of isobaric signals.
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Aggregate and Fragment Analysis in Therapeutic Monoclonal Antibodies Using On-Line Size-Exclusion Chromatography with Native Mass SpectrometrySize-exclusion chromatography (SEC), with the use of ammonium acetate buffer, can be coupled on-line to electrospray ionization MS for the characterization of size variants of therapeutic monoclonal antibodies (mAbs). A quadrupole time-of-flight (QTOF) MS system was employed, and the MS method was optimized to achieve favorable sensitivity for high-mass detection, while maintaining the structural integrity of the aggregates (or high molecular weight species) and fragments (or low molecular weight species).
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Stability and Recovery Influences of Benzo[a]pyrene, Benzo[a]anthracene, Benzo[b]fluoranthene, and Chrysene during Sample Preparation of Plant MatricesQuantification of European Union (EU)-priority polycyclic aromatic hydrocarbons (PAHs) in plant matrices is a crucial task. Various methods for enrichment and preconcentration, such as the preloaded-pipette tip solid‑phase extraction (SPE) (1), are available. Nevertheless, analyte recovery as a result of homogenization, sample preparation, and extraction are rarely discussed in the field of phytopharmacy. This study deals with the recovery in dry plant extracts, which are typically used in phytopharmaceuticals and reflect the actual polycyclic aromatic hydrocarbon content in the commercially available end product (2). The aim of this study was to monitor benzo[a]pyrene, benzo[a]anthracene, chrysene, and benzo[b]fluoranthene loss of spiked samples as a result of commonly-used sample pretreatment, extraction, filtering, and evaporating techniques in 1:1 (v/v) cyclohexane–ethyl acetate primulae flos and sambuci flos dry extracts. Results showed that improper sample preparation can lead to false results. In the case of benzo[a]pyrene with a deviation of 155% from the theoretical true value.
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Mass Spectrometry Support to Mitigate Product Recall Situations in the Pharmaceutical IndustryThese case studies illustrate how the pharmaceutical industry utilizes mass spectrometry–based solutions to address product quality concerns, particularly when extremely low detection limits are required and when development resources are limited.
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Analytical Techniques in Exploring the Persistent Global PFAS ProblemThe presence of PFAS in the environment requires governmental agencies to establish regulatory limits for PFAS in human essentials, such as drinking water. LC–MS/MS can be used to analyze a wide range of sample types containing PFAS, but avoiding PFAS contamination is critical for this technique to work effectively.
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Turbulent-Flow LC for LC–MS and LC–MS–MS BioanalysisThis month's "Directions in Discovery" gives a brief overview of turbulent- flow liquid chromatography, a technique for fast separations. Two examples demonstrate the use of the technology for high-throughput bioanalytical separations in drug discovery.
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When van Deemter Goes Upside Down in Chiral ChromatographyThe occurrence of convex-upward van Deemter curves is rare but not unusual in chiral liquid chromatography. In this work, this behaviour, experimentally observed for the more retained enantiomer of a chiral sulfoxide on a polysaccharide-based CSP, is explained.
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Simultaneous Analysis of Ultrashort-Chain, Alternative, and Legacy PFAS in Potable and Non-Potable WatersA hybrid HILIC–ion exchange column was used for the analysis of ultrashort-chain per- and polyfluoroalkyl substances (PFAS) compounds in environmental waters. This direct injection LC–MS method enables simultaneous measurement of ultrashort- chain, alternative, and legacy PFAS in potable and non-potable waters.
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How Much Data is Too Much? An Analysis of the Pros and Cons of High-Resolution Mass Spectral DataHRMS is widely used in chromatography for analyzing complex mixtures. However, chromatographers should take note of the exponentially larger data set that HRMS provides before deciding whether to use the technique for the analysis they are conducting.
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Method Development for Reversed-Phase Separations of Peptides: A Rational Screening Strategy for Column and Mobile Phase Combinations with Complementary SelectivityThis review article summarizes the results obtained from the combined efforts of a joint academic and industrial initiative to solve the real-life challenge of determining low levels of peptide-related impurities in the presence of the related biologically-active peptide at a high concentration.
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Method Development for Reversed-Phase Separations of Peptides: A Rational Screening Strategy for Column and Mobile Phase Combinations with Complementary SelectivityThis review article summarizes the results obtained from the combined efforts of a joint academic and industrial initiative to solve the real-life challenge of determining low levels of peptide-related impurities in the presence of the related biologically-active peptide at a high concentration.
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Evaluating Two Dioscin-Based Silica Stationary Phases and their Application to Achiral and Chiral SeparationsImproved analysis of pharmaceutical and natural medicine products requires advances in reversed-phase LC stationary phases. We examine two synthesized stationary phases with applicability in quality control and chiral separation for analysis of natural products.
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Indirect Suppressed-Conductivity Ion ChromatographyThe authors demonstrate a new detection method for various anions of weak acids separated by ion chromatography and point out the main advantages of this method.
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Introducing PSG® Biotech (Aug 2023)PSG® Biotech offers a comprehensive portfolio of pumps, sensors and flow meters engineered to protect media integrity and product quality while producing the highest yields – from laboratory to commercial scale.
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Py-GC-MS Investigation of Pyrolysis Behaviours and Decomposition Products of α- and β-2,7,11-Cembratriene-4,6-DiolsThe study of the thermal behaviour and pyrolysis products of these terpenoids could possibly suggest flavour precursors that could be used to provide specific flavours to tobacco.
Latest:
Nontargeted Screening for the Verification of Allergenic Ingredients and Perfume Authenticity by GC-ecTOF-MSIn this study, the experimental nontargeted screening approach and corresponding data analysis workflows—simultaneously using molecular ion information and structural information—are presented for the molecular identification and authenticity verification process from a brand perfume using GC–ecTOF-MS.
Latest:
Turbulent-Flow LC for LC–MS and LC–MS–MS BioanalysisThis month's "Directions in Discovery" gives a brief overview of turbulent- flow liquid chromatography, a technique for fast separations. Two examples demonstrate the use of the technology for high-throughput bioanalytical separations in drug discovery.
Latest:
Multi-Active Method (MAM) for the Analysis of Agriculture Product Technical Ingredients and Formulated ProductsA collaborative multi-analyte method developed utilizes HPLC and UHPLC to analyze more than 70 active ingredients. This method offers a validated approach for determining technical AI alongside linearity, precision, accuracy, and specificity tests for seven active ingredients.
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The Use of Molecular Imprinted Polymers Prior to Chromatographic Methods for the Analysis of Bisphenols in Packaged FoodsWe examine the rapid growth of green microextraction techniques, such as molecular imprinted polymer solid-phase extraction (MISPE), in the field of food quality and safety, and how this technique is used for bisphenol A (BPA) analysis.
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Analysis of Boronic Compounds as Potential Mutagenic Impurities in Drug Substances By GC–MSThis novel gas chromatography (GC)–MS approach enables analysis of the suspected mutagens bis(pinacolato)diboron (BPD) and tetrahydroxydiboron (BBA) found at low-levels in certain intermediates and active pharmaceutical ingredients (APIs), even in the presence of boron from other sources.
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Validation of Stability-Indicating HPLC Methods for Pharmaceuticals: Overview, Methodologies, and Case StudiesIn the pharmaceutical industry, method validation is essential. But what are the best practices? We review regulatory requirements, validation parameters, methodologies, acceptance criteria, trends, and software tools.
