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Developing a short list of likely causes of retention-time problems in reversed-phase LC makes troubleshooting easier.

Essentials of LC Troubleshooting, Part II: Misbehaving Retention Times

A kinetic plot is a powerful tool, but how do you construct one—from either experimental data or data from other sources? We explain.

But Why Doesn’t It Get Better? Kinetic Plots for Liquid Chromatography, Part II: Making and Interpreting the Plots

When applying Golay’s equation for height equivalent of a theoretical plate (HETP) to capillary GC, you should ask three key questions.

Is Golay’s Famous Equation for HETP Still Relevant in Capillary Gas Chromatography? Part 2: Assumptions and Consequences

Barry L. Karger and James P. Grinias are the winners of the 15th annual LCGC Lifetime Achievement and Emerging Leader in Chromatography Awards, respectively, for 2022. Here, we review their achievements.


The 2022 Winners of the Lifetime Achievement and Emerging Leader in Chromatography Awards

Kinetic plots can help us understand how different combinations of parameters will perform in relation the time needed to acquire a particular column efficiency—and thus resolution.

But Why Doesn’t It Get Better? Kinetic Plots for Liquid Chromatography, Part I: Basic Concepts

We are all familiar with Golay’s equation relating to height equivalent to a theoretical plate (HETP) in GC. But do we understand it correctly?

Is Golay’s Famous Equation for HETP Still Relevant in Capillary Gas Chromatography? Part 1: A Common View of HETP

Are we ready to let software and instruments take over the art of separations?

Don’t Fear the Automation

This article explains how statistical overlap theory can be applied to chromatography in everyday usage.

The Simple Use of Statistical Overlap Theory in Chromatography

The statistical overlap theory (SOT) of chromatography relates the number of peaks that appear in a chromatogram to the number of detectable components and the peak capacity.

Despite the utility of high performance liquid chromatography (HPLC) simulators, we found that all the free and low-cost simulators were outdated or had extremely limited functionality, so we created one that addressed these shortcomings. We developed a sophisticated, open-source HPLC simulator that is available for free as well as a version for Android users called "HPLC Simulator." Here we discuss a few questions that the simulator can help answer: Why are peaks narrower in gradient elution? What is gradient delay and how does it affect a separation?

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