Capillary Electrophoresis

The advantages of a native anion exchange method coupled to mass spectrometry for charge heterogeneity analysis of immunoglobulin G4 (IgG4)-based mAbs are described.

At Pittcon 2024, LCGC International editor Patrick Lavery sat down with Wolfgang Lindner of the University of Vienna to talk about his career in liquid chromatography (LC).

A recent study examined how capillary electrophoresis (CE) could identify new 4-aryl-pyrido[1,2-c]pyrimidine derivatives in human serum.

Wolfgang F. Lindner and Martina Catani are the winners of the 17th annual LCGC Lifetime Achievement and Emerging Leader in Chromatography Awards, respectively. These awards honor the work of talented separation scientists at different stages in their career. The award winners will be honored in March in an oral symposium at the Pittcon 2024 conference in San Diego, California.

This month we spoke to Zhuoheng Zhou from the Vrije Universiteit Brussel in Belgium about his research on advancing instrumental and column technologies for modern chromatography and its applications in novel biopharmaceutical modalities and proteomics.

Chromatography is predicted to be a major player among instrument technologies, but spectroscopy and electrophoresis may also increase their market shares.

Microfluidic capillary electrophoresis mass spectrometry (CE-MS) using ZipChip technology provides intact mass evaluations and efficient separation of oligonucleotides.

In this study, an efficient technique is described for extracting and quantifying barbiturates in serum samples, combining ultrasound and surfactant-assisted dispersive liquid–liquid microextraction (dLLME) with poly(ethylene oxide)-mediated stacking in capillary electrophoresis.

Professor Mary J. Wirth, W. Brooks Fortune Distinguished Professor Emerita at Purdue University, has been named the winner of the 2023 EAS Award for Outstanding Achievements in Separation Science.

An upcoming study revealed more information about the folding dynamics of i-motif DNA structures.

A recent study enhances capillary electrophoresis by streamlining the preparation process for linear polyacrylamide (LPA)-coated capillaries.

Researchers investigated a three-dimensional fluorinated covalent organic framework (3D-FCOF) as a stationary phase in capillary electrochromatography.

Microfluidic devices are considered ideal for fabricating portable diagnostic equipment for point-of-care testing, but substrates more environmentally friendly than glass or plastics are attracting interest.

Scientists at NACRW discussed using CE–MS to analyze highly polar agrochemicals.

Researchers have synthesized a chiral metal-organic framework (MOF) called L-histidine-zeolitic imidazolate framework-67 (L-His-ZIF-67) for enantioseparation of drugs in capillary electrochromatography. The chiral MOF demonstrated excellent separation efficiency for five chiral drugs, offering promising possibilities for pharmaceutical analysis and quality control.

Representatives of three subclasses of flavonoids were targeted for identification and quantitation in non-destructive single seed evaluation of Medicago sativa and Melilotus officinalis.

Researchers have discovered that the combined use of deep eutectic solvents (DESs) and amino acid-based ionic liquids (AAILs) with cyclodextrin-based chiral selectors can significantly enhance the chiral separation of amphetamine derivatives.

New MSPE-CE-DAD study indicates that efficiency remained above 75% even when a magnetic solid-phase extraction (MSPE) method was repeated five times, noting that the magnetic porous carbon (MPC) used in the extraction can be easily recycled.

A newly published review reveals insights into the history of capillary electrophoresis, from its development to its current state-of-the-art applications, through a comprehensive bibliometric analysis of publications between 1980 and 2021.

Scientists propose a gel-free hybridization assay using peptide nucleic acid (PNA) probes and capillary electrophoresis (CE) for qualitative and quantitative analysis of nucleic acid therapeutics.

An examination of capillary gel electrophoresis (CGE) and a look at where the technique is heading in the future is presented in this review.

A new method using capillary electrophoresis to determine sulfur oxide could have far-reaching effects on the wine industry.

Jeffrey Dick received the 2023 Pittcon Achievement Award for his contributions to the field of spectroscopy. He was recognized at Pittcon 2023, which took place in Philadelphia, Pennsylvania.

Barry L. Karger and James P. Grinias are the winners of the 15th annual LCGC Lifetime Achievement and Emerging Leader in Chromatography Awards, respectively, for 2022. Here, we review their achievements.

In this paper, two adeno-associated virus (AAV) genome integrity analysis workflows are introduced; a standard sample preparation protocol and an accelerated procedure, both utilizing capillary gel electrophoresis with laser induced fluorescent detection (CGE–LIF) to analyze the released nucleic acids.

The complexity and challenges of developing, manufacturing, and controlling cell therapies offer today’s chromatographic scientists new opportunities to join the journey of discovery and innovation needed to develop and commercialize this new drug modality. This article explores the latest developments and highlights the importance of new capillary electrophoresis–mass spectrometry (CE–MS)-based approaches to understand the efficiency of cell therapy production.

This is the second in a series of articles exploring current topics in separation science that will be addressed at the HPLC 2019 conference in Milan, Italy, from 16–20 June.

Analytical separation techniques based on the differential migration velocities of analytes under the action of an electric field are gaining increasing acceptance for the analysis of phenolic compounds in edible and medicinal plants and in plant-derived food products. In Part 2 of this review article the authors discuss the fundamental principles and practical aspects of electromigration techniques, including capillary zone electrophoresis (CZE), micellar electrokinetic chromatography (MEKC), and capillary electrochromatography (CEC). The development of two-dimensional systems, performed by coupling either liquid chromatography (LC) with an electromigration technique or two electromigration techniques, operated under different separation mechanisms, is also discussed.

The analytical toolbox used in present-day metabolomics encounters difficulties for the analysis of limited amounts of biological samples. Therefore, a significant number of crucial biomedical and clinical questions cannot be addressed by the current metabolomics approach. Capillary electrophoresis–mass spectrometry (CE–MS) has shown considerable potential for the profiling of polar and charged metabolites in volume-restricted or mass-limited biological samples. This article considers advances that significantly improved the performance of CE–MS for in-depth metabolic profiling of limited sample amounts. Attention is also devoted to various technical aspects that still need to be addressed to make CE–MS a viable approach for volume-restricted metabolomics.