Application Notes: Size-Exclusion Chromatography (SEC)
Assessing Protein Aggregation with SEC-MALS
Determine the molecular weight of low abundant species with confident with the highly sensitive DAWN MALS detector and improved peak separation with Waters BEH Column.
Analysis of mAbs, biosimilars and AAVs
Explore SEC-MALS-RI multi-attribute quantification of antibodies, biosimilars, and AAVs.
SEC-MALS and Fluorescence detection of AAV
Explore the optimization of AAV separation and the combined intrinsic fluorescence detection with SEC-MALS for low-concentration measurement.
AEX-MS analysis of an IgG4-based mAb
In this application note, the charge variant characterisation of an IgG4-based mAb was performed using a BioPro IEX QF anion exchange column coupled to an MS detector.
GPC/SEC-Viscometry for Structural Analysis
GPC/SEC-viscometry enables measurement of true molar masses for carrageenan and detects subtle differences between samples with similar molar mass distributions.
GPC/SEC-MALLS Analysis of Hyaluronic Acid (Aug 2023)
GPC/SEC-MALLS analysis of hyaluronic acid obtains absolute molar mass while conventional GPC/SEC with concentration detectors only provides apparent molar masses.
Light Scattering and Size Exclusion Chromatography
Discover how SEC can be used in biopolymer applications including bioconjugates, proteins, mAbs, mRNA1, viruses, extracellular vesicles, and liposomal nanoparticles.
Towards Greener GPC/SEC
This paper discusses sustainable solutions for size separation of macromolecules that align with the 12 principles of green chemistry.
GPC/SEC Applications - Polymers, Biopolymers, Proteins
Tips & Tricks designed to support GPC/SEC users in their daily work, providing comprehensive overviews on different aspects of this powerful technique.
Saccharide and Polysaccharide Analysis (Aug 2023)
Robust, reliable GPC/SEC analysis of saccharides and polysaccharides can occur with very high resolution using Agilent SUPREMA columns with 3 and 5 µm particles.