Application Notes

Articles

Learn the importance of light scattering detection to determine the true molecular weight distribution of a poly(glutamic acid)-based copolymer for drug-delivery. 

SEC-MALS Characterization of a Drug-delivery Polymer

This application note introduces application data obtained by using FlexFire SEC Columns that have adopted newly developed superior silica.


Compound Separation with Size-Exclusion Chromatography

Shodex™ has successfully developed the LB-800 columns which are suitable with MALS detection. The columns are well suited to provide signal at low concentration with low molecular weight standards. 

Absolute Molecular Weight Determination of Natural Polymers Using ShodexTM OHpakTM LB-806M and MALS Detection 

Shodex™  has SB-800 HQ series for the analysis of water-soluble molecules and for the determination of molecular weight distribution.

New Shodex™  OHpak™  LB-800 HQ GFC Columns for Low Baseline Noise MALS Analysis

This technical article introduces an example of multi-faceted analysis and monitoring of an EV sample purification process using a SEC column, Shodex™ OHpak SB-806 HQ, with 3 different detectors.


Shodex™ OHpak SB-806 HQ SEC Multi-Faceted Analysis of Exosome (EV) Combination of UV, Florescence, and Light Scattering Detectors

When selecting a suitable analytical SEC column, not only the analytical range for the fractionation, but adsorption of the target compounds, which influences the quantification and yield, should also be considered. 

Purification and SEC/MALS Analysis of Norovirus Virus-Like Particles Comparison Using the ShodexTM OHpak SB-805 HQ

This application note explains how SEC coupled with MALS can be used for the determination of structural changes in polysaccharides. 


Monitoring Structural Changes in Polysaccharides Using SEC-MALS

This application note introduces some new application data obtained by using FlexFire SEC that is adopted by newly developed silica gel.





SEC Columns for Large and Small Molecules

Field-flow fractionation coupled to multi-angle light scattering (FFF-MALS) is a powerful analytical tool that determines multiple CQAs of LNP-RNA nanoparticles.

Characterizing Lipid Nanoparticles (LNPs) for RNA Delivery

SEC with multi-angle light scattering (SEC-MALS) or in conjunction with a viscometer (SEC-MALS-IV) effectively characterizes branching in polymers.

Application Notes: Size-Exclusion Chromatography (SEC)