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© 2022 MJH Life Sciences and Chromatography Online. All rights reserved.
© 2022 MJH Life Sciences™ and Chromatography Online. All rights reserved.
April 13, 2022
Field-flow fractionation with online static and dynamic light scattering constitutes a powerful and versatile system for nano-pharmaceutical characterization.
March 11, 2022
The SEC separation of recombinant human growth hormone somatotropin with good peak shape and separation of high molecular weight aggregate was observed.
Protein standards explored with known hydrodynamic radii, show how this can determine ideal size-exclusion conditions for optimal method performance using Biozen columns.
This application note explores the effect of column internal diameter (4.8 and 7.6 mm IDs) for robust size-exclusion chromatography methods.
Exploring the use of a standard phosphate containing buffer for aggregate analysis of an IgG2 mAb, Panitumumab, which has known recovery issues due to its hydrophobicity
Size-exclusion method improvements by running at a lower flow rate for the aggregate analysis of NIST mAb using a Biozen dSEC-2 column
The robustness of a sub-2 µm Biozen dSEC-2 column is demonstrated by displaying over 300 injections of a protein sample (Myoglobin).
This application, using a Biozen dSEC-2 column, demonstrates the effect of organic solvent of an ADC “mimic" on the analysis.
NIST mAb was analyzed using six different batches of 1.8 µm 200 Å dSEC-2 media packed with 150 mm length and 4.6 mm inner diameter columns.
SEC–HRMS demonstrated for the analysis of an IdeZ digested mAb for specific identification of any posttranslational modifications specific to the hypervariable region.