Application Notes: Size-Exclusion Chromatography (SEC)

Liposome Structure and Concentration by FFF-MALS-DLS

April 13, 2022

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Field-flow fractionation with online static and dynamic light scattering constitutes a powerful and versatile system for nano-pharmaceutical characterization.

Aggregate Analysis of Recombinant Human Growth Hormone v2

March 11, 2022

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The SEC separation of recombinant human growth hormone somatotropin with good peak shape and separation of high molecular weight aggregate was observed.

White Paper: Size-Exclusion Chromatography (SEC) for the Determination of Protein Hydrodynamic Radius

March 11, 2022

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Protein standards explored with known hydrodynamic radii, show how this can determine ideal size-exclusion conditions for optimal method performance using Biozen columns.

The Effect of Column Internal Diameter for Robust SEC Methods

March 11, 2022

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This application note explores the effect of column internal diameter (4.8 and 7.6 mm IDs) for robust size-exclusion chromatography methods.

Aggregate Analysis of an IgG2 Monoclonal Antibody Using a Biozen dSEC-2 LC Column

March 11, 2022

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Exploring the use of a standard phosphate containing buffer for aggregate analysis of an IgG2 mAb, Panitumumab, which has known recovery issues due to its hydrophobicity

Improving Resolution for Size-Exclusion Chromatography Methods

March 11, 2022

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Size-exclusion method improvements by running at a lower flow rate for the aggregate analysis of NIST mAb using a Biozen dSEC-2 column

Assessing Column Lifetime of a UHPLC Biozen dSEC-2 LC Column

March 11, 2022

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The robustness of a sub-2 µm Biozen dSEC-2 column is demonstrated by displaying over 300 injections of a protein sample (Myoglobin).

Organic Solvent in Size-Exclusion Chromatography of an Antibody Drug Conjugate Surrogate

March 11, 2022

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This application, using a Biozen dSEC-2 column, demonstrates the effect of organic solvent of an ADC “mimic" on the analysis.

Batch-to-Batch Comparison of Sub-2 µm Diol-Linked Size-Exclusion Columns

March 11, 2022

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NIST mAb was analyzed using six different batches of 1.8 µm 200 Å dSEC-2 media packed with 150 mm length and 4.6 mm inner diameter columns.

SEC–High Resolution Mass Spectrometry (HRMS) for IdeZ Digested Monoclonal Antibody

March 11, 2022

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SEC–HRMS demonstrated for the analysis of an IdeZ digested mAb for specific identification of any posttranslational modifications specific to the hypervariable region.