Application Notes: Size-Exclusion Chromatography (SEC)

Characterizing Branched Polymers by Light Scattering

September 15, 2022

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SEC with multi-angle light scattering (SEC-MALS) or in conjunction with a viscometer (SEC-MALS-IV) effectively characterizes branching in polymers.

Understanding Antibody and Viral Glycoprotein Interactions (Sept 2022)

September 15, 2022

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SEC-MALS and CG-MALS characterize viral glycoprotein (vGP) self-association and the absolute stoichiometry and affinity of vGP: antibody interactions.

Batch-to-Batch Comparison of Sub-2 µm Diol-Linked Size-Exclusion Columns (August 2022)

August 18, 2022

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NIST mAb was analyzed using six different batches of 1.8 µm 200 Å dSEC-2 media packed with 150 mm length and 4.6 mm inner diameter columns.

White Paper: Size-Exclusion Chromatography (SEC) for the Determination of Protein Hydrodynamic Radius (August 2022)

August 18, 2022

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Protein standards explored with known hydrodynamic radii, show how this can determine ideal size-exclusion conditions for optimal method performance using Biozen columns.

Improving Resolution for Size-Exclusion Chromatography Methods (August 2022)

August 18, 2022

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Size-exclusion method improvements by running at a lower flow rate for the aggregate analysis of NIST mAb using a Biozen dSEC-2 column

Thermal Extraction of VOCs Using a Multi-ramp Method

August 08, 2022

This application note demonstrates a quick temperature ramp technique to improve the focusing of VOCs without a need for trapping.

Characterizing Vaccines with Light Scattering (August 2022)

August 08, 2022

Light scattering methods inform on essential properties and attributes of vaccines including size, aggregation, stability, interactions, composition, and conformation.

Characterization of Adeno-Associated Viral Vectors

June 08, 2022

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Quality attributes of Adeno-Associated Virus (AAV) vectors are achieved with multi-angle light scattering coupled to separation methods and high-throughput dynamic light scattering.

Protein Aggregate Analysis by SEC

June 06, 2022

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mAb and ADC aggregate analysis by SEC is introduced. Separation was improved by suppressing secondary interactions using high concentrations of corrosive salts.

Liposome Structure and Concentration by FFF-MALS-DLS

April 13, 2022

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Field-flow fractionation with online static and dynamic light scattering constitutes a powerful and versatile system for nano-pharmaceutical characterization.