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A novel LC–MS/MS method, with a dual ESI and APCI source using simple solvent extraction for sample preparation, was validated for analysis of 102 pesticides and 5 mycotoxins regulated by Colorado state in dried hemp.

Novel Liquid Chromatography–Tandem Mass Spectrometry Method for Analysis of 102 Pesticides and Five Mycotoxins Regulated by the State of Colorado in Dried Hemp

Articles

Two-dimensional gas chromatography (GC×GC) and chemometrics are leading the way in new strategies on multivariate data analysis. Using GC×GC in untargeted analysis reveals how far the technique has advanced the field of separation science.

Revisiting the Fundamentals of Untargeted Data Analysis with Comprehensive Two-Dimensional Gas Chromatography (GC×GC): With Great Peak Capacity, There Must Also Come Great Responsibility

A column with chemically modified column hardware showed improvements in analytical performance for siRNA compared to a conventional stainless-steel column.

Analysis of siRNA with Denaturing and Non-Denaturing Ion-Pair Reversed-Phase Liquid Chromatography Methods

Christina Jayne Vanhinsbergh is a researcher at the University of Sheffield, UK. Her Ph.D. research focused on the development of multidimensional chromatographic methods for the analysis of therapeutic oligonucleotides. Currently, she is researching DNA alkylation from environmental compounds using LC–MS methodologies. Christina is the membership officer for the Chromatographic Society, UK.

This article reviews a range of techniques available for separative analysis of mRNA therapeutics, their associated impurities, and delivery vehicles.

The Role of Separation Techniques in the Analysis of mRNA Therapeutic Drug Substances and Drug Products

A harmonization of all conventional hold-up volume methods was successfully applied to RPLC, HILIC, AEX, and RP-AEX mixed-mode chromatography, regardless of the mobile phase.

Harmonization of Experimental Methods Used to Measure the True Hold-Up Volume of Liquid Chromatography Columns

Online monitoring of odour and taste components that occur at parts-per-trillion (ppt) levels in industrial process waters requires specialized analytical hardware that is generally not compatible with the harsh environmental conditions in these typical industrial settings. An alternative instrumental method is proposed that uses dynamic extraction in combination with gas chromatography (GC) equipped with a simple flame ionization detector (FID) to achieve these extremely low detection limits.

Online Solid-Phase Extraction–Gas Chromatography–Flame Ionization Detection System for Monitoring Contaminants at Parts-Per-Trillion Concentrations in Process Waters

The methodologies demonstrated here open the way to fully compliant at-line monitoring of monoclonal antibody quality attributes.

New 2D-LC–MS Approaches for the Analysis of In-Process Samples and for the Characterization of mAbs in a Regulated Environment

Pepsin digestion and guanidine hydrochloride post-digestion can improve sequence coverage in antibody peptide mapping compared with trypsin digestion.

Overcoming Incomplete Peptide Mapping of Antibody Complementarity-Determining Regions with Alternate Digestion Workflows

The method presented here, which uses a novel needle trap device (NTD)
with sol-gel PEG-coated fibers in conjunction with GC, is a simple, inexpensive, and accurate tool for the analysis of trace levels of PCBs in water samples.

Determination of Polychlorinated Biphenyls in Water Samples Using a Needle Trap Device Combined with Gas Chromatography

Jun Liu is a graduate master’s student in the Department of Chemistry, Liaoning Normal University.

Qianfeng Weng is an associate professor in the Department of Chemistry, Liaoning Normal University. He received his doctoral degree in analytical chemistry from Shandong University, China, in 2001.

Jinxiang Li received his doctoral degree in analytical chemistry in 2002 from Gunma University, Japan, and became a full professor in 2003 at Liaoning Normal University, China. His research interests include the development of micro-columns for liquid chromatography and capillary electrochromatography, and interfacing devices for the coupling of the separation techniques with other analytical techniques.

This article presents a new hydrophilic SAX hybrid monolithic column prepared by in‑capillary coating 5 μm bare silica particles with functional organic polymers. After preparation conditions were investigated and selected, the resulting column was characterized in detail.

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