June 12th 2023
Adjustable polyacrylamide stationary phases developed by researchers enhance separation performance in liquid chromatography, according to a recent publication. The study demonstrates the synthesis of vinyl modified silica and the successful preparation of polyacrylamide modified silica (PAM-SIL) stationary phases, allowing for fine control of separation performance.
New 2D LC–MS Analytical Method Unveils the Secrets of Ancient Chinese Herbal Formula HGWD
October 10th 2023A comprehensive analytical approach utilizing online two-dimensional liquid chromatography coupled with hybrid linear ion trap orbital trap mass spectrometry, has enabled the in-depth analysis of Huangqi Guizhi Wuwu decoction, identifying 170 chemical constituents and offering insights into its complex chemical composition and potential therapeutic properties.
Polysorbate Analysis in Biopharmaceutical Applications—A Snapshot of the Analytical Toolbox
An overview of different approaches for the qualitative and quantitative analysis of polysorbates.
Strategies for Overcoming Sample Introduction Challenges in Hydrophilic-Interaction Chromatography
July 13th 2023A team of researchers has investigated strategies to overcome sample introduction challenges in hydrophilic-interaction chromatography (HILIC). By employing techniques such as pre-column dilution and selecting alternative organic solvents, they successfully addressed the issues related to injection solvent strength, resulting in improved peak symmetry and separation efficiency in HILIC analyses.
Successful Analysis of Protein Biopharmaceuticals Using Ultra-Short Columns
June 20th 2023Davy Guillarme of the University of Geneva said in a talk at HPLC 2023 that short columns have shown promise, but ultrahigh-performance liquid chromatography (UHPLC) instrumentation is not yet optimized to realize their benefits.
An LC–MS/MS Method for the Evaluation of Intestinal Permeability in Blood Plasma
May 1st 2023A rapid and robust LC–MS/MS method for determining lactulose and rhamnose concentrations in blood plasma was used to determine intestinal permeability from blood plasma, which can help diagnose gastrointestinal diseases such as Crohn’s disease.
New Nickel Oxide Stationary Phase for Improved HILIC Separation
April 27th 2023A new study has reported the preparation, characterization, and chromatographic evaluation of a nickel oxide deposited silica stationary phase in HILIC, which exhibited multiple retention mechanisms and enhanced chromatographic selectivity for analytes.
Clinical Metabolomics: Expanding the Metabolome Coverage Using Advanced Analytical Techniques
September 1st 2019This review article discusses the novel separation and detection strategies that are considered promising in clinical metabolomics to enhance the metabolome coverage. It includes hydrophilic interaction chromatography (HILIC), supercritical fluid chromatography (SFC), multidimensional LC approaches, as well as ion-mobility mass spectrometry (IM-MS) and data-independent acquisition (DIA) analysis methods.
Hydrophilic Interaction Liquid Chromatography: An Update
March 1st 2019This article is an update on the technique of HILIC and covers recent ideas on the mechanism of separation, and how it may be manipulated to suit the separation of particular sample types. The advantages of HILIC are discussed, and also the actual and perceived disadvantages of the technique and how the latter can be overcome. Some new applications of HILIC for characterization of biopharmaceuticals, where it can even be applied to the separation of intact proteins, and to applications in metabolomics, will be discussed.
Combatting Counterfeit Biopharmaceuticals Using Hydrophilic Interaction Chromatography
November 12th 2018The Column spoke to Steven Janvier, a PhD student at Sciensano and Ghent University (Belgium), and Celine Vanhee, a scientist at Sciensano, about their work to develop a hydrophilic interaction chromatography (HILIC) methodology capable of detecting counterfeit polar peptide drugs available on the black market.
Analyzing Artificial Sweeteners as Environmental Contaminants
June 21st 2018The Column spoke to Núria Fontanals, a senior researcher at the Department of Analytical and Organic Chemistry, Universitat Rovira i Virgili, Spain, about her work investigating the impact of artificial sweeteners as environmental pollutants using hydrophilic interaction chromatography (HILIC) and the broader role of HILIC in environmental analysis.
Direct Analysis of Amino Acids by HILIC–ESI-MS
July 1st 2017Hydrophilic interaction liquid chromatography coupled to electrospray ionization mass spectrometry (HILIC–ESI-MS) has been established as a method to separate and quantify polar and ionic analytes in a direct way for two decades. HILIC separation is based on the polarity of analytes, so the more polar analytes have stronger retention on a HILIC column.