June 28th 2024
In biotherapeutics manufacturing, controlling size variants of therapeutic monoclonal antibodies (mAbs) is critical for ensuring product quality.
Quantitative Metrics to Properly Describe Solute Elution in Size-Exclusion Chromatography
February 16th 2024The purpose of this short tutorial article is to review the terms and official nomenclatures for size-exclusion separations and to provide some guidance and recommendations for practicing chromatographers. The interconversion between the different metrics is explained and some examples are presented.
Calibrating the Low Molar Mass Range in GPC/SEC Using Oligomer Peaks
February 15th 2024Reliable calibration curves in the low molar mass region can be obtained when using the elution volumes of all separated oligomers of low molar mass standards on high resolution columns. This article explains how to do this.
New Strategies for Oligonucleotide Aggregate Analysis
January 15th 2024Technological limitations have made it difficult for scientists to identify oligonucleotide aggregates. This can pose challenges when finding techniques to reliably analyze size variants for large nucleic acids and lipid nanoparticles (LNPs).
The LCGC Blog: Polymers, Macromolecules, and Nanomaterials in the Separation Science Curriculum
August 2nd 2021In 2015, the American Chemical Society’s Committee on Professional Training added a requirement to the ACS degree certification program that undergraduates learn about macromolecules, supramolecular aggregates, and nanomaterials (MSN). This requirement can be met by a specialized course in these topics, but many programs are also choosing the distribute these topics across the curriculum.