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Determination of molar mass distributions and the molar mass averages derived therefrom are the main objectives of gel permeation chromatography/size-exclusion chromatography (GPC/SEC) analysis. But what is the meaning of these averages and how are they influenced by the setting of baselines and integration limits? This instalment of Tips and Tricks in GPC/SEC will try to provide a better understanding.


Tips & Tricks GPC/SEC: A Look at Molar Mass Averages and How They Are Affected By SEC Baseline and Integration Settings

This installment of Tips & Tricks will provide some helpful pointers to keep
your GPC/SEC system in good shape.

Tips & Tricks: Treating Your GPC/SEC System Properly

The state of protein-derived self-associated, aggregated, and fragmented impurities in therapeutics are critical quality attributes (CQAs) and are widely monitored using non-denaturing size-exclusion chromatography (SEC).

Modern Size-Exclusion Chromatography Separations of Biosimilar Antibodies at Physiological pH and Ionic Strength

Developing and building a talent base in macromolecular separations should be a priority for employers. Here’s why.

Whence the Next Generation of Macromolecular Separations Scientists?

Heidi Berg is a trained chemical laboratory technician and has been working in PSS’s contract analysis department on GPC/SEC since 2003.

What experimental details need to be considered when analyzing PEGs?

Tips & Tricks: Trouble Analyzing PEGs?

In 2015, the American Chemical Society’s Committee on Professional Training added a requirement to the ACS degree certification program that undergraduates learn about macromolecules, supramolecular aggregates, and nanomaterials (MSN). This requirement can be met by a specialized course in these topics, but many programs are also choosing the distribute these topics across the curriculum.

The LCGC Blog: Polymers, Macromolecules, and Nanomaterials in the Separation Science Curriculum

This exploration into commercially available light scattering detection systems for size-exclusion chromatography (SEC) emphasizes new technologies and will help users understand the options.

Chromatography Fundamentals, Part X: Light Scattering Detection Systems for Size-Exclusion Chromatography

Sophia Kenrick, PhD,* is an application scientist at Wyatt Technology Corporation, 6300 Hollister Ave, Santa Barbara, CA 93117-3253.

Quantifying AAV Quality Attributes Using SEC–MALS

This instalment describes the interplay between column length, pore size distribution, and particle size to optimize GPC/SEC separations.

Tips & Tricks GPC/SEC: Separation Range and Resolution

Hydrolyzed collagens (collagen peptides) are water-soluble products obtained by hydrolysis of natural proteins and used for dietary supplements. A simple GPC/SEC method is described for molar mass determination of collagen peptides, allowing reliable molar mass determination using ultraviolet (UV) detection.

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