Application Notes

Articles

Advance your oligonucleotide work, from sample preparation to analysis and purification.
 


Clarity Biosolutions for Synthetic DNA/RNA Guide v2

This technical note discusses 2' cleavage and RNA sample preparation prior to trityl-on Clarity QSP cartridge or high-throughput purification.

 


RNA Sample Preparation and High Throughput Purification for TBDMS and TOM Chemistries v2

Using the Clarity OTX extraction protocol vendor recommendations, we have demonstrated its use to develop a method for the extraction of therapeutic oligos from plasma.

 


Extraction of 2’-MOE Phosphorothioate from Plasma Using Clarity OTX-v2

Common frequently asked question for oligonucleotides. This includes questions about characterization to purification and from sample preparation to LC separation.



FAQs for the Isolation and Purification of Synthetic Oligonucleotides v2

Clarity QSP provides an alternative trityl-on approach for separating truncated sequences and synthetic contaminants from the protected full-length sequence. By incorporating a polymeric sorbent with a unique buffer formula, aborted fragments and synthetic contaminants are removed during sample loading offering a departure from the traditional RPC mechanism. Multiple buffers and gradient elutions are eliminated, thereby making Clarity QSP a 3-step product for trityl-on oligonucleotide purification.

 


Comparing Performance of High-Throughput, Trityl-on RNA/DNA Purification Products v2

As synthetic oligonucleotide therapeutics move through the drug-development path, the need for TK and PK/PD studies in drug discovery and development has increased. 


Automated Method Development of Oligonucleotide in Tissues Using Clarity OTX 96-Well Plate and High Resolution Mass Spectrometry

Simple, quick, effective solution for isolating and analyzing therapeutic oligonucleotides using Clarity OTX, an extraction kit based on mixed-mode ion-exchange SPE.

 


Extraction and LC–MS Analysis of Oligonucleotide Therapeutics from Biological Matrices Using Clarity® OTX™ SPE and Clarity Oligo-MS™ HPLC/UHPLC Columns

To ensure recovery and reproducibility for oligo extraction from tissue, it is imperative that an effective sample pretreatment strategy is used.
 


Extraction of 2’-MOE Phosphorothioate from Tissue Using Clarity OTX Solid-Phase Extraction (SPE)

Trityl-on methodologies for the analysis of crude synthetic oligonucleotides has many benefits however depurination during trityl-on methodologies can lead to nucleic acid damage. This technical note explores a new trityl-on procedure that minimizes the risk of depurination.


 


Avoiding Depurination During Trityl-on Purification v2

Extraction of Oligo therapeutics from biological samples for LC/MS bioanalysis.


Application Notes: General