Application Notes: General

BUCHI continues to drive innovation in chromatography. To help you unlock brilliance in your lab, BUCHI launched the Pure Excellence Chromatography System, a new flash (and optional prep HPLC) instrument designed for ease of use, advanced precision, and enhanced safety. Curious to know how you can improve high-performance purifications? Join BUCHI specialists, Alina Gau and Birke Götz, as they showcase how the new Pure Excellence Chromatography System simplifies your pharma and chemistry R&D workflows.

The product portfolio includes the C-905, C-910, and C-915 for flash applications, offering flexible detection options, including ELSD. The C-950 further expands application capabilities by integrating prep HPLC, providing added versatility for laboratories with advanced purification requirements.Building on BUCHI’s long-standing reputation for reliability and innovation, the Pure Excellence Chromatography Systems combine user-friendly operation with high separation performance. Key features include: A quick-release cartridge holder enabling secure, one-handed cartridge installation to simplify daily operation. Automated and manual sample injection modes supporting both liquid injection and solid sample loading for improved reproducibility and time efficiency. A high-performance pump with a three-piston design ensures pulse-free flow. Evaporative Light Scattering Detection (ELSD) offers near-maintenance-free operation and high detection accuracy through patented direct nano-pulse injection technology. Four-solvent gradient capability expands method development flexibility and simplifies challenging separations. Advanced safety features, including continuous system monitoring, active ventilation with a closed fraction collector (enabling operation outside a fume hood), and RFID-based fraction collector rack identification. Intuitive software with method/run preview, simplified gradient editing, and always visible run monitoring supports faster, more consistent purification. With a focus on simplicity, performance, and safety, the Pure Excellence Chromatography Systems enable laboratories to achieve purification goals faster and with greater confidence.

See how the Pure Excellence Chromatography System makes purification faster, safer, and easier than ever. In this product demo, we walk through every step of running a chromatographic separation, from priming the solvent lines to collecting your final fractions.

This study investigates the separation of egg white proteins using flash and prep HPLC with silica stationary phases varying in pore and particle sizes. Larger pore sizes improved protein interaction with the stationary phase, while particles above 10 µm were ineffective. Optimal separation was achieved using the Pure Excellence C-950 with a PrepPure C18WP column, combining wide pores, small particles, and a step gradient.

Comprehensive characterization of biotherapeutics requires advanced analytical strategies capable of detecting and identifying low-abundant product quality attributes (PQAs), including post-translational modifications, aggregates, sequence variants, and conjugation sites. This eBook presents three technical articles demonstrating advanced LC-MS workflows that achieve up to 10X improvement in MS sensitivity for trace-level detection and confident identification of these challenging attributes.

Discover a next generation laboratory balance designed for connected, automated environments. This article explains how network ready weighing technology supports regulatory compliance, digital workflows, and seamless integration into robotics and lab IT systems, enabling smarter data handling and more efficient, reproducible laboratory processes.

This white paper summarizes harmonized pharmacopeial requirements for analytical balances across USP, Ph.Eur., JP, and ChP. It explains regulatory timelines, calibration and performance check expectations, environmental control recommendations, and practical guidance for meeting accuracy and repeatability criteria in pharmaceutical quality control laboratories.

This note applies the EPA 1633 workflow to fish and animal tissues, enabling sensitive, low‑level PFAS detection using automated SPE and LC/MS/MS. The dual‑layer SPE design improves efficiency and recovery, meeting all EPA performance criteria across complex tissue matrices.

In food safety testing, instrument reliability under real-world conditions is the true test of performance. This technical note showcases the durability and consistency of the PerkinElmer QSight® 500 LC/MS/MS system through an intensive robustness study, analyzing high-matrix food samples with minimal cleanup and no loss in sensitivity. Using a simplified QuEChERS extraction (no SPE), the system successfully analyzed PFAS analytes across complex matrices like salmon, avocado, pet food, and spices. The results highlight not only long-term stability but also the power of StayClean™ technology to reduce downtime and keep throughput high.

This application note presents a simplified Blend‑and‑Inject LC‑MS/MS method for the quantification of 25 PFAS compounds in animal‑derived food matrices using the QSight® 500 LC‑MS/MS system. By eliminating SPE and leveraging matrix‑matched calibration, isotopically labeled standards, and a PFAS delay column, the method achieves EU‑compliant sensitivity and robust performance in complex tissues, enabling high‑throughput and reliable PFAS monitoring for food safety compliance.

Learn how water quality, handling, purification technologies, final filters, and maintenance practices help ensure optimal LC-MS performance and reliability

Measure ethylene oxide and other air toxics using US EPA Method 327, achieving 10 ppt detection limits without liquid cryogen.

Methodology for assessing PFAS emissions from food contact materials under typical usage conditions using dynamic headspace sampling and TD-GC-MS/MS.

Understand the consumer aroma experience of fragrance-release products with automated time-resolved VOC sampling

Automated pairwise chromatogram comparison identifies key differences between GC-MS and GC×GC-MS data in minutes, simplifying data processing and reporting.

Large-volume extraction with TD-GC-MS enables reproducible, mass-based measurement of microplastics across wastewater treatment stages.

Enhance SPME sensitivity using multi-step enrichment for improved VOC and SVOC profiling and more effective short-duration sampling.

BioAccord LC-MS System with ACQUITY Premier - Product Brochure

Optimizing sample preparation is critical for generating high-quality, reproducible analytical data, particularly when working with complex matrices. Yet, it often demands significant time and effort. Traditional methods, including solid phase extraction (SPE), supported liquid extraction (SLE), filtration, and nucleic acid (NA) extraction, rely on column- or plate-based protocols and can face limitations in throughput, reproducibility, and the handling of difficult samples. Conventional approaches such as centrifugation and vacuum manifolds introduce further challenges, from added manual intervention and inconsistent flow to difficulty processing viscous samples. Positive pressure instrumentation uses controlled gas flow to push liquids through columns or plates, maintaining constant pressure across all positions to improve analyte recovery, reduce matrix effects, and support automation. This whitepaper examines how positive pressure compares to conventional methods and how Tecan's Resolvex platforms apply the technology across manual, semi-automated, and fully automated workflows.

See how IC-ICP-MS differentiates Cr(III) and Cr(VI) in water with reliable separation, sensitive detection, and streamlined software control.

This application note explores the use of positive pressure processing for plasmid DNA extraction by automating key preparation steps in a large-volume purification workflow. Performed in a compact benchtop format, this automated approach helps improve consistency, reduce hands-on time and streamline plasmid DNA purification for molecular biology and genomics applications.

This application note demonstrates an automated workflow for the analysis of trypsin-digested bovine serum albumin (BSA), combining on-column digestion and peptide clean-up using solid phase extraction (SPE) within a single column. Using automated positive pressure processing, the workflow streamlines sample preparation, reduces hands-on time and delivers consistent, reproducible peptide recovery for downstream analysis.

This poster highlights an enhanced workflow for automated monoclonal antibody (mAb) purification using positive pressure processing with AffinEx™ Protein A SPE columns ranging from 1 ml to 50 ml. The study evaluates purification performance across multiple column sizes, demonstrating a scalable approach that helps laboratories improve throughput, maintain consistency and streamline mAb purification from development through larger-scale applications.

This webinar recording provides a practical introduction to positive pressure processing for sample preparation, highlighting its advantages in achieving more consistent flow rates, improving reproducibility and reducing variability across samples. Learn how positive pressure technology can support reliable, high-quality results while streamlining everyday sample preparation workflows.

This poster explores automated and semi-automated workflows that leverage positive pressure processing for small-volume sample preparation using micro-elution plates. The study evaluates how positive pressure technology can improve flow consistency, maximize analyte recovery from limited sample volumes and deliver the reproducible performance needed for accurate and reliable analytical results.

Gas chromatography is a powerful tool for volatile and semi-volatile species in battery materials, but its application faces significant challenges. Many electrolyte components and degradation products are thermally labile or highly reactive, leading to on-column decomposition and poor quantification.

Discover how the new PDMS/HLB and PDMS/DVB/CWR GERSTEL Twister® coatings expand chemical coverage beyond traditional PDMS for GC-MS profiling of lemon–lime beverages. The study compares extraction performance, repeatability, and formulation differentiation using multivariate analysis, helping laboratories select the optimal sorbent phase for flavor and formulation studies.

Explore how GERSTEL Vacuum In-Tube Extraction (V-ITEX) enables highly reproducible profiling of volatile chocolate aromas. This application demonstrates automated extraction, PCA-based product differentiation, and reliable quality control across multiple chocolate formulations, making V-ITEX an ideal solution for food and flavor analysis.