Application Notes: General

Per‑ and polyfluoroalkyl substances (PFAS) are persistent environmental contaminants that can accumulate in animal‑derived foods, creating growing regulatory and public health concerns. Traditional PFAS testing methods often rely on time‑consuming solid‑phase extraction (SPE) and extensive cleanup to manage complex tissue matrices. This application note demonstrates a simplified, high‑throughput Blend & Inject LC‑MS/MS workflow using the PerkinElmer QSight® 500 LC‑MS/MS system for reliable PFAS quantification in animal‑derived food products while meeting stringent EU and EPA regulatory requirements.

PFAS rules now reach the packaging itself. Under the EU Packaging and Packaging Waste Regulation, food contact packaging containing 25 ng/g or more of any individual PFAS cannot be placed on the market, so labs need targeted data at production volume. This application note shows a Direct Shoot LC-MS/MS method on the PerkinElmer QSight 500 system that quantifies 26 PFAS in food packaging bottles, pharmaceutical packaging bottles and packaged drinking water. It uses a simple methanol extraction and no SPE cleanup. Calibration runs from 0.05 to 25 ng/g, with recoveries predominantly within 70 to 130%.

Semaglutide and tirzepatide are now household names, but behind every dose sits an analytical problem that small-molecule thinking can’t solve. Isomers share a mass, impurities hide in the tail of the main peak, and peptides cling to tubing, vials, and column hardware. Characterizing GLP-1 Therapeutics: Analytical Challenges and Solutions explores how separation scientists are responding. An expert Peer Exchange panel weighs up orthogonal methods, 2D-LC, inert hardware, and the role of AI in peptide method development. We trace the peptide revolution from Gila monster venom to a global surge in manufacturing capacity. And a new isocratic HPLC–DAD method shows that stability testing for both blockbuster peptides can be done in just three minutes.

Semaglutide and tirzepatide are now household names, but behind every dose sits an analytical problem that small-molecule thinking can’t solve. Isomers share a mass, impurities hide in the tail of the main peak, and peptides cling to tubing, vials, and column hardware. Characterizing GLP-1 Therapeutics: Analytical Challenges and Solutions explores how separation scientists are responding. An expert Peer Exchange panel weighs up orthogonal methods, 2D-LC, inert hardware, and the role of AI in peptide method development. We trace the peptide revolution from Gila monster venom to a global surge in manufacturing capacity. And a new isocratic HPLC–DAD method shows that stability testing for both blockbuster peptides can be done in just three minutes.

Biopharmaceutical analysis is being asked to keep pace with increasingly complex molecules, from monoclonal antibodies to oligonucleotides, lipid nanoparticles, and RNA therapeutics, while method development itself becomes faster and more automated. In Advances in Biopharmaceutical Analysis, LCGC International brings together leading researchers tackling that challenge: revisiting the history, principles, and growing applications of affinity chromatography, exploring how AI and robotics are reshaping method development in the autonomous laboratory, automating the Separation Quality Factor (SQF) within chromatography data systems for objective method evaluation, and combining solid-phase enzymatic digestion with porous graphitic carbon chromatography for rapid LC–MS analysis of RNA modifications.

Biopharmaceutical analysis is being asked to keep pace with increasingly complex molecules, from monoclonal antibodies to oligonucleotides, lipid nanoparticles, and RNA therapeutics, while method development itself becomes faster and more automated. In Advances in Biopharmaceutical Analysis, LCGC International brings together leading researchers tackling that challenge: revisiting the history, principles, and growing applications of affinity chromatography, exploring how AI and robotics are reshaping method development in the autonomous laboratory, automating the Separation Quality Factor (SQF) within chromatography data systems for objective method evaluation, and combining solid-phase enzymatic digestion with porous graphitic carbon chromatography for rapid LC–MS analysis of RNA modifications.

Food analysis rarely stands still: limits keep falling, supply chains keep growing, and more sensitive instruments keep finding compounds nobody was looking for. RAFA 2026 Companion: Hot Topics in Food Analysis previews the conference in Prague (3–6 November). It opens with an introduction from symposium chairs Stefan van Leeuwen and Jana Pulkrabova, followed by interviews with five RAFA speakers. Darina Dvořáková covers changing EU PFAS rules, and Franz Berthiller makes the case for retention indices in mycotoxin LC–MS. Michele Suman screens alkaloids in cereals and spices, Anneli Kruve ranks unknowns in non-target screening by risk, Nicolai Zederkopff Ballin defines tolerable levels in food authentication, and Laura Righetti looks at the strengths and limits of mass spectrometry imaging.

APP NOTE: Advantage of HALO 1000A OLIGO C18 siRNA Impurity Analysis

APP NOTE: Rapid Analysis of Mivelsiran Using HALO 1000 Å OLIGO C18

TECHNICAL REPORT- IMPROVED OLIGONUCLEOTIDE ANALYSIS USING HALO® OLIGO C18

HALO®OLIGO Guidebook

See how combustion ion chromatography can be used to determine total organic fluorine in textiles as manufacturers respond to evolving PFAS requirements. This application proof note demonstrates a C-IC workflow using the Cindion Combustion Ion Chromatography System and Dionex Inuvion IC system.

PFAS testing requirements vary by matrix and analytical goal. See workflows spanning sample preparation, targeted LC-MS/MS analysis, combustion ion chromatography, high-resolution mass spectrometry and data processing for water, soil and other challenging samples. The guide also compares approaches for known PFAS quantitation and unknown screening.

See how Professor Lee Ferguson's laboratory at Duke University combines high-resolution mass spectrometry, Compound Discoverer software and advanced cheminformatics to investigate complex PFAS mixtures. The work includes a spectral library of more than 46,000 known and predicted compounds to support identification of known, unexpected and emerging PFAS.

Get an overview of PFAS sources, environmental pathways and analytical approaches across industrial and environmental applications. This roadmap connects routine targeted testing with broader investigational workflows, including sample preparation, LC-MS/MS, combustion ion chromatography and high-resolution mass spectrometry.

Explore a semi-automated approach to PFAS analysis in soil designed to meet US EPA Method 1633A performance requirements. The workflow automates extraction and sample clean-up to increase throughput, reduce manual handling and help minimize opportunities for error and PFAS contamination.

Explore the use of adsorbable organic fluorine and combustion ion chromatography to screen for PFAS in wastewater. This application note presents results from the EPA Method 1621 multi-laboratory collaboration study, including practical guidance for adsorption, combustion, ion chromatography and method implementation.

Dive into the wonderful world of fluidic fittings and chromatography. Whether you're a curious newbie or just need a refresher, our newly revamped FITTINGS 101 Guide is your backstage pass to mastering the art of making perfect connections in the lab

Strengthen your chromatography platform. From the degasser to the detector and everything in between, view high-quality components that offer ease of use and enable high-resolution chromatography separations. With a comprehensive range of quality components and connections, you can optimize your fluidic pathway for the lowest delay volumes and maximize sensitivity.

When it comes to high-precision tubing, you want a supplier who is fully equipped to construct to your exact specifications, saving you time and complexity. This brochure highlights extrusion, cutting and forming, testing, and labeling every piece, so you receive a single assembly that’s ready to integrate directly into your system.

MarvelXACT is a connection system for trouble-free liquid chromatography. It has been expertly designed to take the guess work out of your process. It eliminates struggles with loose components as seen with other fittings on the market. It has no loose components such as nuts or Ferrell's, and it requires no tools. All you need to do is finger tighten until the first clicked feedback is received. This click feedback confirms optimum torque has been reached and installation is complete. It is that simple!

Help increase confidence in polysorbate quantification with a solution designed for complex biopharmaceutical samples. In this application note, learn how the Waters™ Charged Aerosol Detector (CAD) delivers sensitive, reproducible quantification of Polysorbate 20 and Polysorbate 80 in monoclonal antibody formulations while simplifying data acquisition, reporting, and compliance through seamless integration with Waters LC systems and Empower™ Software.

Recycled PET manufacturers need to prove their packaging is free of unwanted volatile contaminants without adding cost or complexity to their QC workflow. Acetaldehyde, benzene, and limonene are the three compounds regulators and converters watch most closely in rPET, and testing for them has traditionally required separate methods on separate instruments. This application note demonstrates a single HS-GC-MS method, using the PerkinElmer GCMS SQ 2400 System and HS 2400M Headspace Sampler, that quantifies all three compounds in one run, with strong precision, linearity, and recovery.

Flexible food packaging protects what's inside it, but the inks and adhesives used to print and laminate it can leave behind residual organic solvents. Those solvents can migrate into food, raising safety concerns and drawing scrutiny under regulations like EU 1935/2004 and GMP requirements in Commission No. 2023/2006. This application note demonstrates how the PerkinElmer GC 2400 System, paired with the HS 2400 M Headspace Sampler, quantifies 16 residual solvents in flexible food packaging with strong precision and accuracy.

Analyze seven common anions in water in just 7.5 minutes using ion chromatography with automated eluent generation and inline ultrafiltration.

Complex water and wastewater matrices can create challenges for routine analytical testing. Explore standardized ion chromatography methods, key ISO and ASTM standards, and analytical workflow considerations for determining ionic and ionizable contaminants across industrial and other water matrices.

Routine, high-throughput laboratories must balance analytical speed, sensitivity, uptime, and reproducibility while managing demanding quantitative workflows. This Instrument Insights explores how the Thermo Scientific™ TSQ Certis Triple Quadrupole Mass Spectrometer (MS) is designed to address these challenges. Ready-to-run workflows and compliance-ready software support can simplify method development and routine operation across clinical research, toxicology, food safety, environmental, and biopharma applications. In addition, performance data demonstrates the robustness in complex matrices and long analytical sequences.

This digital supplement explores the analytical hurdles laboratories face as PFAS testing pushes into trace-level territory, from a chemical universe spanning millions of structures to persistent background contamination and non-harmonized methods across regulatory bodies. Featured research covers non-targeted screening strategies for identifying unsuspected PFAS in complex food matrices, advanced multisorbent extraction and mass spectrometry workflows for organofluorines in municipal wastewater, and findings that PFAS concentrations in estuaries can shift four- to sevenfold within a single tidal cycle. Additional coverage details the development of fluorinated SPME sorbents for selective extraction of volatile PFAS and a fast GC-MS/MS method capable of detecting 37 PFAS compounds in food packaging in just 8 minutes. Together, these articles offer a data-driven look at emerging tools and techniques designed to keep pace with PFAS's expanding chemical scope and evolving regulatory demands.