Application Notes: Medical/Biological

Here we present an innovative approach for the characterization of nanoplastic particles using centrifugal field-flow fractionation coupled with UV, multi angle light Scattering detection, and raman microscopy.

The timsTOF fleX system bridges a current gap by providing MALDI Imaging and in-depth proteomics analysis in just one instrument. The instrument offers all benefits of a timsTOF Pro for time-efficient and sensitive proteomics, combined with a high-resolution MALDI source and stage. Using PASEF technology, it is possible to retrieve high protein ID rates with small sample amounts. Here we present the new SpatialOMx® workflow to identify distinct proteomic profiles for different tumor subpopulations in breast cancer as an example for this powerful approach.

Abuse of synthetic opioid prescription painkillers such as fentanyl, along with a rapidly growing list of illicit analogues, is a significant public health problem. In this study, we developed a simple dilute-and-shoot method that provides a fast 3.5-min analysis of fentanyl and related compounds (norfentanyl, acetyl fentanyl, alfentanil, butyryl fentanyl, carfentanil, remifentanil, and sufentanil) in human urine by liquid chromatography–tandem mass spectrometry (LC–MS/ MS) using a Raptor Biphenyl column.

Antisense DNA and siRNA are widely used for gene silencing in research and medical applications. An effective delivery of the oligonucleotides into cells is important for clinical applications.

This application note demonstrates a multistep thermal extraction study on tetrahydrocannabinol (THC) vape oils.

Amyloid β proteins are highly hydrophobic and can form aggregates. The analysis of 4 Aβ using YMC-Triart Bio C4 with larger pore and lower hydrophobicity is presented.

Demonstratation of the glycopeptide enrichment efficiency of iSPE®-HILIC material for glycoproteomics applications using a well characterized glycoprotein standard.

In this study, AF4-MALS-ICP-MS was applied to characterize four commercial tattoo inks: outlining black, purple deep violet, green grasshopper, and blue ice.

In this study, we developed a simple dilute-and‑shoot method that provides a fast 3.5-min analysis of fentanyl and related compounds (norfentanyl, acetyl fentanyl, alfentanil, butyryl fentanyl, carfentanil, remifentanil, and sufentanil) in human urine by LC–MS/MS using a Raptor Biphenyl column.

Sphingolipids are major components forming biological membranes, and they serve as intracellular signalling molecules. It is important to measure the amount of these molecules in biological samples because they have a massive influence on various metabolic diseases such as obesity, diabetes, and Alzheimer’s disease. Recently, an improved method using a YMC-Triart C18 metal-free column was reported by Dr. Gowda et al.

Abuse of synthetic opioid prescription painkillers such as fentanyl, along with a rapidly growing list of illicit analogues, is a significant public health problem. In this study, we developed a simple dilute-and‑shoot method that provides a fast 3.5-min analysis of fentanyl and related compounds in human urine by LC–MS/MS using a Raptor Biphenyl column.

etermination of oligomeric states is an important issue in protein chemistry. For example, self-assembly via oligomerization domains is crucial for the regulation of several protein kinases. Determination of the oligomeric state of fragments of these kinases is a means of verifying the involvement of each domain in self-assembly.

Determination of oligomeric states is an important issue in protein chemistry. For example, self-assembly via oligomerization domains is crucial for the regulation of several protein kinases. Determination of the oligomeric state of fragments of these kinases is a means of verifying the involvement of each domain in self-assembly.

In this study, a reversed-phase HPLC method was developed for separation of five steroid hormones with partition coefficients ranging from 1.47 (cortisone) to 4.5 (pregnenolone) on a Hamilton PRP-C18 HPLC column.