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Analysis of unidentified organofluorine compounds and known PFAS in environmental and human samples presents many challenges. Combustion ion chromatography is one technique that can help.

Analyzing Organofluorine Compounds in the Environment Using Combustion Ion Chromatography (CIC) and Other Methods

Robert MacNeill is Head of Method Development at LabCorp Drug Development.
He received his bachelor’s degree with honours in chemistry from Heriot-Watt University, and his M.Sc. in analytical chemistry from the University of Huddersfield, UK. Robert is also a Chartered Chemist and Fellow of the Royal Society of Chemistry, and has 24 years of experience in all aspects of quantitative bioanalytical LC–MS method development—with 15 of these spent heading method development activities within LabCorp Drug Development at the Somerset (New Jersey, USA) site. Taking a prominent role in innovation projects with related R&D activities, Robert has been an active contributor to the scientific literature for many years.

Using Earth as a figurative analogy, we explore the merits of flexibility when considering which liquid chromatography (LC) modality to use for any particular bioanalysis.

Pursuing the Polar Routes with Penguins and Polar Bears: Balancing the Choice of Chromatographic Mode

Interest in three-dimensional (3D) printing technology is expanding rapidly. What impact can it have in mass spectrometry? Robert Winkler, of the Center for Research and Advanced Studies Irapuato, in Guanajuato, Mexico, is exploring this question. We recently spoke with Prof. Winkler about this work.

Three-Dimensional Printing in Ion Mobility and Mass Spectrometry

Modern ion mobility–mass spectrometry (IM–MS) is a key separation technology for detailed molecular characterization studies and also as part of emerging data acquisition strategies for demanding small molecule and several applications. Here is what you need to know.

Ion Mobility–Mass Spectrometry (IM–MS): Enhancing Performance of Analytical Methods

Using ion mobility, analytes that have the same molecular mass can be separated by their shape, centers of mass, and collision cross section, but challenges such as ion loss can still occur. A new development in ion mobility separation, high-resolution ion mobility (HRIM), addresses such problems, and is particularly well suited to challenging applications, such as glycosylation monitoring of biological drugs and vitamin D analysis.

Six Key Differentiators Between Liquid Chromatography and High-Resolution Ion Mobility

Ion-exchange chromatography (IEX) is a frequently overlooked technique. This novel IEX method uses non-aqueous mobile phases to purify a non-crystalline intermediate after a reductive amination with D-xylose. IEX delivers a step change in strength and purity of the intermediate allowing successful downstream processing.

Non-aqueous Ion-Exchange Chromatography Using High Acid-base Concentration: A Strategy for Purifying Non-crystalline Pharmaceutical Intermediates

LCGC Announces the 2021 Winners of the Lifetime Achievement and Emerging Leader in Chromatography Awards

Sulphite is a preservative added to a vast range of foods and beverages to prevent browning or oxidation. Some individuals are sensitive to sulphite additives and may experience a range of allergic reactions. Therefore, both the U.S. Food and Drug Administration (FDA) and European Union (EU) laws require that the presence of sulphites be declared on food labels when the concentration exceeds 10 mg/L. Several analytical methods exist to measure sulphite in food and beverages, however, they suffer from repeatability issues, and can be quite cumbersome to perform. A patent has been filed for an innovative, fast, and accurate ion chromatographic (IC) method based on direct current (DC) mode electrochemical detection. This article explains more.

A Simplified Method to Determine Total Sulphite Content in Food and Beverages via Ion Chromatography

Characterization of mAbs and related products requires the identification of chromatographic peaks with MS. However, the conventional salt- and pH-gradient elution techniques used in IEX are inherently incompatible with MS. Ammonium acetate- and ammonium carbonate-based mobile phase systems have been recently applied in IEX-MS, but the influence of the eluent composition on peak shape and retention has not been discussed nor studied systematically until now. The aim of the present study was to understand the impact of ionic strength, buffer capacity, and pH-response on the retention behaviour and peak shape of mAb species.

Optimization of MS-Compatible Mobile Phases for IEX Separation of Monoclonal Antibodies

Ion-pairing chromatography has traditionally been implemented with the addition of an ion-pairing reagent into the mobile phase. Here, an alternative method is presented, in which the ion-pairing reagent is deposited on column as a sample additive instead of in the mobile phase.

Ion Chromatography | Topics